Experiment set3S518 for Rhodopseudomonas palustris CGA009

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Sodium L-Lactate carbon source with 10 mM Sodium Chloride

Group: stress
Media: PM + Sodium L-Lactate (13 mM) + Sodium Chloride (10 mM) + Light intensity (12 µmol photons/m2/s from a 60-W incandescent light bulb)
Culturing: RPal_CGA009_ML8, tube, Anaerobic, at 30 (C)
By: Rpal_McKinlay on 9/8/24
Media components: 12.5 mM Disodium phosphate, 12.5 mM Potassium phosphate monobasic, 1 g/L Ammonium Sulfate, 0.1 mM Sodium thiosulfate pentahydrate, 0.002 g/L 4-Aminobenzoic acid, UW concentrated base (0.02 g/L Nitrilotriacetic acid, 0.0289 g/L Magnesium sulfate, 0.00667 g/L Calcium chloride dihydrate, 1.85e-05 g/L ammonium molybdate tetrahydrate, 0.000698 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L EDTA, 0.001095 g/L Zinc sulfate heptahydrate, 0.000154 g/L Manganese (II) sulfate monohydrate, 3.92e-05 g/L Copper (II) sulfate pentahydrate, 2.5e-05 g/L Cobalt(II) nitrate hexahydrate, 1.77e-05 g/L sodium tetraborate decahydrate)

Specific Phenotypes

For 26 genes in this experiment

For stress Sodium L-Lactate in Rhodopseudomonas palustris CGA009

For stress Sodium L-Lactate across organisms

SEED Subsystems

Subsystem #Specific
Methionine Biosynthesis 2
Murein hydrolase regulation and cell death 2
Anaerobic respiratory reductases 1
Arsenic resistance 1
Cysteine Biosynthesis 1
DNA-replication 1
DNA repair, bacterial 1
Folate Biosynthesis 1
Glutathione: Non-redox reactions 1
Heat shock dnaK gene cluster extended 1
High affinity phosphate transporter and control of PHO regulon 1
Methylglyoxal Metabolism 1
Phosphate metabolism 1
Ribosome biogenesis bacterial 1
Sulfur oxidation 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-cysteine biosynthesis I 2 2 1
arsenic detoxification (bacteria) 4 3 2
arsenate detoxification III 2 1 1
pseudouridine degradation 2 1 1
methylglyoxal degradation VIII 3 3 1
NAD salvage pathway III (to nicotinamide riboside) 3 2 1
methylglyoxal degradation I 3 2 1
arsenate detoxification I 6 3 2
UTP and CTP dephosphorylation I 7 5 2
purine nucleotides degradation II (aerobic) 11 7 3
inosine 5'-phosphate degradation 4 3 1
guanosine nucleotides degradation II 4 3 1
guanosine nucleotides degradation III 4 3 1
adenosine nucleotides degradation I 8 5 2
purine nucleotides degradation I (plants) 12 7 3
guanosine nucleotides degradation I 4 2 1
adenosine nucleotides degradation II 5 3 1
seleno-amino acid biosynthesis (plants) 5 3 1
hydrogen sulfide biosynthesis II (mammalian) 6 4 1
superpathway of guanosine nucleotides degradation (plants) 6 3 1
arsenic detoxification (plants) 6 3 1
superpathway of purines degradation in plants 18 8 3
ureide biosynthesis 7 3 1
superpathway of methylglyoxal degradation 8 3 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
tunicamycin biosynthesis 9 1 1
Rubisco shunt 10 10 1
nucleoside and nucleotide degradation (archaea) 10 1 1
NAD salvage (plants) 11 4 1
arsenic detoxification (yeast) 12 3 1
Calvin-Benson-Bassham cycle 13 12 1
oxygenic photosynthesis 17 13 1
superpathway of seleno-compound metabolism 19 8 1
ethene biosynthesis V (engineered) 25 20 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 20 1
1-butanol autotrophic biosynthesis (engineered) 27 21 1