Experiment set3IT094 for Dyella japonica UNC79MFTsu3.2
Glucuronamide nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Glucuronamide (20 mM), pH=7
Culturing: Dyella79_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 3.7 generations
By: Adam on 17-May-17
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1768 C6
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source Glucuronamide in Dyella japonica UNC79MFTsu3.2
For nitrogen source Glucuronamide across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Auxin degradation | 1 |
| Oxidative stress | 1 |
| Photorespiration (oxidative C2 cycle) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Methane metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- Phenylpropanoid biosynthesis
- Biosynthesis of phenylpropanoids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| betanidin degradation | 1 | 1 | 1 |
| superoxide radicals degradation | 2 | 2 | 1 |
| isoniazid activation | 2 | 2 | 1 |
| methanol oxidation to formaldehyde IV | 2 | 1 | 1 |
| baicalein degradation (hydrogen peroxide detoxification) | 2 | 1 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| reactive oxygen species degradation | 4 | 4 | 1 |
| xanthommatin biosynthesis | 4 | 1 | 1 |
| luteolin triglucuronide degradation | 4 | 1 | 1 |
| sesamin biosynthesis | 8 | 1 | 1 |
| matairesinol biosynthesis | 10 | 1 | 1 |
| justicidin B biosynthesis | 10 | 1 | 1 |