Experiment set3IT091 for Pseudomonas fluorescens FW300-N2E2

outer cut, LB soft agar motility assay

Group: motility
Media: LB + Agar (3 g/L)
Culturing: pseudo6_N2E2_ML5, Agar plate, Aerobic, at 30 (C), shaken=0 rpm, (Solid)
By: Mark on 3/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 44 genes in this experiment

For motility Agar in Pseudomonas fluorescens FW300-N2E2

For motility Agar across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	11
Bacterial Chemotaxis	6
Flagellar motility	6
Two-component regulatory systems in Campylobacter	3
ABC transporter oligopeptide (TC 3.A.1.5.1)	2
Bacterial Cytoskeleton	1
Bacterial hemoglobins	1
Benzoate transport and degradation cluster	1
Biogenesis of c-type cytochromes	1
Flagellum in Campylobacter	1
HMG CoA Synthesis	1
Leucine Degradation and HMG-CoA Metabolism	1
Periplasmic disulfide interchange	1
Plasmid replication	1
Protein chaperones	1
Proteolysis in bacteria, ATP-dependent	1
Terminal cytochrome C oxidases	1
Transcription initiation, bacterial sigma factors	1
Two cell division clusters relating to chromosome partitioning	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Oxidative phosphorylation
• Purine metabolism
• Caffeine metabolism
• Valine, leucine and isoleucine degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arsenite to oxygen electron transfer	2	1	1
urate conversion to allantoin I	3	2	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	3	1
ferrichrome A biosynthesis	5	3	1
L-leucine degradation I	6	5	1
Fe(II) oxidation	6	3	1
ureide biosynthesis	7	6	1
superpathway of purines degradation in plants	18	14	1