Experiment set3IT091 for Methanococcus maripaludis S2

Compare to:

Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE

200 most detrimental genes:

  gene name fitness t score description  
MMP_RS01370 +2.0 16.4 acetate--CoA ligase family protein compare
MMP_RS03215 +1.6 13.5 MBL fold metallo-hydrolase compare
MMP_RS07115 +1.5 9.5 MBL fold metallo-hydrolase compare
MMP_RS07355 +1.1 2.4 transcription factor S compare
MMP_RS01650 +0.9 1.6 sulfurtransferase TusA family protein compare
MMP_RS05535 +0.8 4.1 hypothetical protein compare
MMP_RS07850 +0.8 3.7 prephenate dehydratase compare
MMP_RS06575 +0.8 4.2 fluoride efflux transporter CrcB compare
MMP_RS07820 +0.8 3.6 UPF0104 family protein compare
MMP_RS03320 +0.7 2.4 metal-dependent transcriptional regulator compare
MMP_RS02605 +0.6 1.1 MotA/TolQ/ExbB proton channel family protein compare
MMP_RS00530 +0.6 3.1 tRNA pseudouridine(54/55) synthase Pus10 compare
MMP_RS02790 +0.6 1.7 class III signal peptide-containing protein compare
MMP_RS04480 +0.6 5.5 lysine 2 3-aminomutase compare
MMP_RS02960 +0.5 4.4 class I SAM-dependent methyltransferase family protein compare
MMP_RS08275 +0.5 4.4 hypothetical protein compare
MMP_RS02675 +0.5 2.1 hypothetical protein compare
MMP_RS08360 +0.5 2.2 hypothetical protein compare
MMP_RS06570 +0.5 2.9 DUF190 domain-containing protein compare
MMP_RS01775 +0.5 2.3 TIGR00297 family protein compare
MMP_RS00665 +0.5 2.6 hypothetical protein compare
MMP_RS07435 +0.5 2.1 GMP synthase subunit A compare
MMP_RS00830 +0.5 2.1 purine phosphoribosyltransferase family protein compare
MMP_RS07365 +0.4 3.4 2-phosphoglycerate kinase compare
MMP_RS06540 +0.4 1.5 3-methyl-2-oxobutanoate dehydrogenase subunit VorB compare
MMP_RS08365 +0.4 2.0 energy conserving hydrogenase EhbF compare
MMP_RS06610 +0.4 2.4 DUF2334 domain-containing protein compare
MMP_RS09025 +0.4 1.0 hypothetical protein compare
MMP_RS03340 +0.4 0.7 ABC transporter ATP-binding protein compare
MMP_RS08345 +0.4 3.1 4Fe-4S binding protein compare
MMP_RS01955 +0.4 1.0 hypothetical protein compare
MMP_RS01540 +0.4 2.3 DUF504 domain-containing protein compare
MMP_RS03520 +0.4 1.5 YwbE family protein compare
MMP_RS04485 +0.4 3.1 putative beta-lysine N-acetyltransferase compare
MMP_RS03420 +0.4 1.6 acetolactate synthase small subunit compare
MMP_RS03670 +0.4 1.1 DUF1890 domain-containing protein compare
MMP_RS05975 +0.4 1.1 hypothetical protein compare
MMP_RS08215 +0.4 1.6 DUF483 domain-containing protein compare
MMP_RS06335 +0.3 2.4 HAD family hydrolase compare
MMP_RS01960 +0.3 0.4 hypothetical protein compare
MMP_RS05700 +0.3 2.6 TfuA-related McrA-glycine thioamidation protein compare
MMP_RS07560 +0.3 1.9 prefoldin subunit alpha compare
MMP_RS06290 +0.3 2.2 DNA repair and recombination protein RadA compare
MMP_RS06555 +0.3 1.8 cupin domain-containing protein compare
MMP_RS08775 +0.3 2.0 methanogenesis marker 9 domain-containing protein compare
MMP_RS08340 +0.3 1.9 4Fe-4S binding protein compare
MMP_RS05315 +0.3 1.1 hypothetical protein compare
MMP_RS07130 +0.3 2.7 coenzyme F420 hydrogenase subunit gamma compare
MMP_RS04740 +0.3 1.5 DUF473 domain-containing protein compare
MMP_RS03390 +0.3 1.9 malate dehydrogenase compare
MMP_RS03790 +0.3 2.3 hypothetical protein compare
MMP_RS01675 +0.3 1.1 hypothetical protein compare
MMP_RS01155 +0.3 2.2 XRE family transcriptional regulator compare
MMP_RS08400 +0.3 1.7 MJ0307 family thioredoxin compare
MMP_RS07120 +0.3 2.2 coenzyme F420 hydrogenase subunit alpha compare
MMP_RS08355 +0.3 1.8 Na(+)/H(+) antiporter subunit B compare
MMP_RS05140 +0.3 1.7 helix-turn-helix transcriptional regulator compare
MMP_RS01975 +0.3 2.5 hypothetical protein compare
MMP_RS05790 +0.3 2.3 HDIG domain-containing protein compare
MMP_RS00285 +0.3 2.2 TIGR04013 family B12-binding domain/radical SAM domain-containing protein compare
MMP_RS05525 +0.3 1.6 calcium/sodium antiporter compare
MMP_RS02370 +0.3 1.5 NifB/NifX family molybdenum-iron cluster-binding protein compare
MMP_RS08260 +0.3 1.5 DUF99 family protein compare
MMP_RS03395 +0.3 1.9 hypothetical protein compare
MMP_RS01580 +0.3 2.2 PQQ-binding-like beta-propeller repeat protein compare
MMP_RS03765 +0.3 1.8 CopG family ribbon-helix-helix protein compare
MMP_RS07090 +0.3 1.9 tRNA (adenine-N1)-methyltransferase compare
MMP_RS08350 +0.3 0.6 hypothetical protein compare
MMP_RS07725 +0.3 1.7 4Fe-4S dicluster domain-containing protein compare
MMP_RS01940 +0.3 0.9 MoxR family ATPase compare
MMP_RS06965 +0.3 2.2 TIGR04014 family B12-binding domain/radical SAM domain-containing protein compare
MMP_RS05980 +0.3 2.1 ferritin compare
MMP_RS01855 +0.3 2.1 2-hydroxyacyl-CoA dehydratase family protein compare
MMP_RS01950 +0.3 2.1 hypothetical protein compare
MMP_RS05985 +0.3 0.9 hypothetical protein compare
MMP_RS02475 +0.3 0.7 hypothetical protein compare
MMP_RS05990 +0.3 1.5 DsrE family protein compare
MMP_RS01630 +0.3 1.8 N-glycosylase/DNA lyase compare
MMP_RS03440 +0.2 0.6 MoaD/ThiS family protein compare
MMP_RS07330 +0.2 2.2 membrane protein compare
MMP_RS07135 +0.2 1.7 coenzyme F420 hydrogenase subunit beta compare
MMP_RS02550 +0.2 1.8 helix-turn-helix transcriptional regulator compare
MMP_RS01150 +0.2 1.1 aldo/keto reductase compare
MMP_RS06860 +0.2 0.7 4Fe-4S binding protein compare
MMP_RS04265 +0.2 1.6 coenzyme F420-reducing hydrogenase FrhD protein compare
MMP_RS06520 +0.2 1.2 DUF1786 domain-containing protein compare
MMP_RS00675 +0.2 0.4 nitroreductase family protein compare
MMP_RS02815 +0.2 0.6 hypothetical protein compare
MMP_RS00100 +0.2 1.9 tRNA pseudouridine(13) synthase TruD compare
MMP_RS04790 +0.2 1.7 4-hydroxy-tetrahydrodipicolinate reductase compare
MMP_RS01945 +0.2 1.6 DUF4350 domain-containing protein compare
MMP_RS05455 +0.2 1.7 DUF167 domain-containing protein compare
MMP_RS05950 +0.2 1.6 nickel-dependent hydrogenase large subunit compare
MMP_RS04520 +0.2 0.7 nucleoside deaminase compare
MMP_RS00655 +0.2 1.2 hypothetical protein compare
MMP_RS01935 +0.2 1.6 DUF58 domain-containing protein compare
MMP_RS08995 +0.2 0.6 hypothetical protein compare
MMP_RS00825 +0.2 1.1 fumarylacetoacetate hydrolase family protein compare
MMP_RS08370 +0.2 0.3 cation:proton antiporter subunit C compare
MMP_RS07815 +0.2 0.6 DUF2120 domain-containing protein compare
MMP_RS08500 +0.2 1.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT compare
MMP_RS02970 +0.2 1.6 hypothetical protein compare
MMP_RS06655 +0.2 1.6 glycoside hydrolase family 15 protein compare
MMP_RS05235 +0.2 1.7 exodeoxyribonuclease III compare
MMP_RS04205 +0.2 1.1 carboxymuconolactone decarboxylase family protein compare
MMP_RS08670 +0.2 1.5 metallophosphoesterase compare
MMP_RS04145 +0.2 0.6 hypothetical protein compare
MMP_RS05970 +0.2 1.2 class II SORL domain-containing protein compare
MMP_RS02705 +0.2 1.4 formylmethanofuran dehydrogenase subunit B compare
MMP_RS07125 +0.2 0.9 coenzyme F420-reducing hydrogenase FrhD protein compare
MMP_RS08075 +0.2 1.3 DUF749 domain-containing protein compare
MMP_RS08480 +0.2 1.6 molybdate ABC transporter substrate-binding protein compare
MMP_RS08455 +0.2 1.1 multiprotein bridging factor aMBF1 compare
MMP_RS06140 +0.2 1.1 radical SAM protein compare
MMP_RS03890 +0.2 1.3 DUF2540 domain-containing protein compare
MMP_RS07100 +0.2 1.5 hypothetical protein compare
MMP_RS02310 +0.2 0.7 RimK-like ATPgrasp N-terminal domain-containing protein compare
MMP_RS02230 +0.2 1.2 sodium-dependent transporter compare
MMP_RS08405 +0.2 1.5 MFS transporter compare
MMP_RS06320 +0.2 1.4 hypothetical protein compare
MMP_RS06910 +0.2 1.2 RlmF-related methyltransferase compare
MMP_RS01420 +0.2 0.8 hypothetical protein compare
MMP_RS02915 +0.2 1.1 amino acid ABC transporter permease compare
MMP_RS08485 +0.2 1.4 tungstate ABC transporter substrate-binding protein WtpA compare
MMP_RS07095 +0.2 1.2 PspC domain-containing protein compare
MMP_RS07955 +0.2 0.9 FumA C-terminus/TtdB family hydratase beta subunit compare
MMP_RS03020 +0.2 1.0 peptidylprolyl isomerase compare
MMP_RS05835 +0.2 0.3 secondary thiamine-phosphate synthase enzyme YjbQ compare
MMP_RS05900 +0.2 1.2 tetratricopeptide repeat protein compare
MMP_RS01030 +0.2 1.3 MTAP family purine nucleoside phosphorylase compare
MMP_RS08380 +0.2 1.3 phosphatase PAP2 family protein compare
MMP_RS02085 +0.2 1.4 inosine/xanthosine triphosphatase compare
MMP_RS08335 +0.2 1.0 NADH-quinone oxidoreductase subunit B family protein compare
MMP_RS08000 +0.2 1.5 methyl coenzyme M reductase-arginine methyltransferase Mmp10 compare
MMP_RS01865 +0.2 0.5 GPR1/FUN34/YaaH family transporter compare
MMP_RS00645 +0.2 1.3 iron ABC transporter permease compare
MMP_RS00955 +0.2 1.3 coenzyme gamma-F420-2:alpha-L-glutamate ligase compare
MMP_RS05695 +0.2 1.2 phospholipase D-like domain-containing protein compare
MMP_RS00540 +0.2 1.1 PLP-dependent aminotransferase family protein compare
MMP_RS01430 +0.2 0.9 mechanosensitive ion channel family protein compare
MMP_RS06325 +0.2 0.5 hypothetical protein compare
MMP_RS01645 +0.2 1.0 SWIM zinc finger family protein compare
MMP_RS03350 +0.2 0.7 winged helix-turn-helix domain-containing protein compare
MMP_RS07890 +0.2 0.5 OB-fold nucleic acid binding domain-containing protein compare
MMP_RS05580 +0.2 1.4 glycosyltransferase compare
MMP_RS03875 +0.2 1.0 hypothetical protein compare
MMP_RS04030 +0.2 1.1 hypothetical protein compare
MMP_RS05660 +0.2 1.2 phosphate ABC transporter substrate-binding protein compare
MMP_RS05945 +0.1 1.0 CBS domain-containing protein compare
MMP_RS00865 +0.1 1.0 SLC13 family permease compare
MMP_RS03950 +0.1 1.0 HNH endonuclease compare
MMP_RS03115 +0.1 1.4 hypothetical protein compare
MMP_RS04960 +0.1 1.2 cytochrome c biogenesis CcdA family protein compare
MMP_RS03675 +0.1 1.3 RimK family alpha-L-glutamate ligase compare
MMP_RS02045 +0.1 0.5 tRNA-Met compare
MMP_RS08700 +0.1 1.1 hypothetical protein compare
MMP_RS02910 +0.1 1.1 ABC transporter substrate-binding protein compare
MMP_RS08205 +0.1 1.1 stage II sporulation protein M compare
MMP_RS01515 +0.1 1.3 PINc/VapC family ATPase compare
MMP_RS05275 +0.1 1.1 AI-2E family transporter compare
MMP_RS08160 +0.1 1.1 EamA family transporter compare
MMP_RS05500 +0.1 1.0 2-isopropylmalate synthase compare
MMP_RS02495 +0.1 0.7 hypothetical protein compare
MMP_RS05960 +0.1 1.2 CoB--CoM heterodisulfide reductase subunit B compare
MMP_RS00795 +0.1 1.1 formate dehydrogenase H subunit alpha selenocysteine-containing compare
MMP_RS02740 +0.1 0.9 hypothetical protein compare
MMP_RS03965 +0.1 0.6 hypothetical protein compare
MMP_RS05770 +0.1 1.0 YkgJ family cysteine cluster protein compare
MMP_RS02570 +0.1 1.2 cation:proton antiporter compare
MMP_RS08640 +0.1 1.2 TIM barrel protein compare
MMP_RS00455 +0.1 1.2 orotate phosphoribosyltransferase-like protein compare
MMP_RS00155 +0.1 0.9 heavy metal-binding domain-containing protein compare
MMP_RS07410 +0.1 1.0 4-demethylwyosine synthase TYW1 compare
MMP_RS08315 +0.1 0.7 S-methyl-5-thioribose-1-phosphate isomerase compare
MMP_RS08470 +0.1 1.0 ATP-binding cassette domain-containing protein compare
MMP_RS01090 +0.1 1.1 ABC transporter substrate-binding protein compare
MMP_RS03760 +0.1 1.1 hypothetical protein compare
MMP_RS03280 +0.1 1.1 pyridoxamine 5'-phosphate oxidase family protein compare
MMP_RS04830 +0.1 1.1 chemotaxis protein CheC compare
MMP_RS01435 +0.1 0.8 DMT family transporter compare
MMP_RS01095 +0.1 0.9 iron ABC transporter permease compare
MMP_RS02360 +0.1 1.0 hypothetical protein compare
MMP_RS00390 +0.1 1.0 ammonium transporter compare
MMP_RS06660 +0.1 1.2 glycosyltransferase family 4 protein compare
MMP_RS08580 +0.1 0.7 flagellin compare
MMP_RS04000 +0.1 1.1 NAD(P)/FAD-dependent oxidoreductase compare
MMP_RS01160 +0.1 0.9 DMT family transporter compare
MMP_RS06375 +0.1 0.8 ATP-dependent sacrificial sulfur transferase LarE compare
MMP_RS01885 +0.1 0.7 Gfo/Idh/MocA family oxidoreductase compare
MMP_RS00385 +0.1 0.9 P-II family nitrogen regulator compare
MMP_RS07185 +0.1 1.0 pyridoxal phosphate-dependent aminotransferase compare
MMP_RS00625 +0.1 0.8 hypothetical protein compare
MMP_RS07620 +0.1 0.5 energy-coupling factor ABC transporter substrate-binding protein compare
MMP_RS05180 +0.1 1.0 Nre family DNA repair protein compare
MMP_RS07415 +0.1 0.9 molybdopterin-guanine dinucleotide biosynthesis protein MobB compare
MMP_RS00670 +0.1 0.6 TIM barrel protein compare
MMP_RS08130 +0.1 0.9 IGHMBP2 family helicase compare
MMP_RS03245 +0.1 0.7 DNA repair and recombination protein RadB compare
MMP_RS08265 +0.1 0.7 rubrerythrin compare
MMP_RS02110 +0.1 0.4 hypothetical protein compare


Specific Phenotypes

None in this experiment

For Methanococcus maripaludis S2 in nutrient t1 experiments

For nutrient t1 Sodium acetate across organisms