Experiment set3IT088 for Pseudomonas fluorescens FW300-N2E2

Compare to:

inner cut, LB soft agar motility assay

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_2625 -5.9 -4.1 Flagellar P-ring protein FlgI compare
Pf6N2E2_75 -5.9 -4.0 Colicin V production protein compare
Pf6N2E2_2585 -5.7 -5.6 Flagellar hook-length control protein FliK conserved
Pf6N2E2_2623 -5.6 -6.7 Flagellar hook-associated protein FlgK conserved
Pf6N2E2_2575 -5.5 -6.5 Flagellar biosynthesis protein FlhF compare
Pf6N2E2_2628 -5.3 -5.1 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_5695 -5.3 -7.2 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_2496 -5.2 -1.8 FIG00956575: hypothetical protein compare
Pf6N2E2_2583 -5.2 -7.1 Flagellar motor switch protein FliM compare
Pf6N2E2_2597 -5.1 -10.4 Flagellar regulatory protein FleQ compare
Pf6N2E2_2317 -5.1 -3.5 Putative analog of CcoH, COG3198 compare
Pf6N2E2_2318 -5.1 -4.9 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_2590 -5.1 -8.4 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_2622 -5.0 -8.4 Flagellar hook-associated protein FlgL conserved
Pf6N2E2_5006 -5.0 -6.8 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_4451 -4.9 -3.4 FIG139438: lipoprotein B compare
Pf6N2E2_2580 -4.9 -3.4 Flagellar biosynthesis protein FliP compare
Pf6N2E2_2624 -4.9 -5.8 Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-) compare
Pf6N2E2_2563 -4.9 -12.2 FIG00953562: hypothetical protein compare
Pf6N2E2_2573 -4.8 -4.7 RNA polymerase sigma factor for flagellar operon conserved
Pf6N2E2_5771 -4.8 -5.8 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_2579 -4.8 -4.7 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_2527 -4.8 -3.3 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_3792 -4.8 -4.7 Glucans biosynthesis protein G precursor compare
Pf6N2E2_5589 -4.8 -7.3 Sodium-type flagellar protein motY precursor conserved
Pf6N2E2_3617 -4.8 -3.3 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_5697 -4.8 -10.7 Flagellar hook protein FlgE compare
Pf6N2E2_2576 -4.7 -12.3 Flagellar biosynthesis protein FlhA compare
Pf6N2E2_5049 -4.7 -2.8 acetyltransferase, GNAT family compare
Pf6N2E2_2627 -4.6 -6.1 Flagellar basal-body rod protein FlgG compare
Pf6N2E2_5150 -4.6 -3.2 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_5963 -4.6 -6.3 lipoprotein, putative conserved
Pf6N2E2_4159 -4.6 -3.2 Cytochrome c4 compare
Pf6N2E2_5337 -4.6 -3.2 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_2593 -4.5 -6.2 Flagellar M-ring protein FliF compare
Pf6N2E2_2428 -4.5 -3.8 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_2569 -4.5 -9.1 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) conserved
Pf6N2E2_2526 -4.5 -5.3 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_2594 -4.5 -6.8 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_2592 -4.4 -6.0 Flagellar motor switch protein FliG compare
Pf6N2E2_5691 -4.4 -8.1 Flagellar basal-body P-ring formation protein FlgA compare
Pf6N2E2_4579 -4.3 -3.0 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_2600 -4.3 -7.8 Flagellar hook-associated protein FliD conserved
Pf6N2E2_4987 -4.3 -1.5 MaoC-like domain protein compare
Pf6N2E2_2582 -4.3 -3.2 Flagellar motor switch protein FliN compare
Pf6N2E2_2823 -4.3 -3.0 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_5252 -4.3 -2.5 FIG00956396: hypothetical protein compare
Pf6N2E2_3791 -4.3 -5.9 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_2710 -4.3 -4.2 FIG00953701: hypothetical protein compare
Pf6N2E2_2320 -4.3 -2.9 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_2567 -4.2 -6.5 Flagellar motor rotation protein MotB conserved
Pf6N2E2_5111 -4.2 -2.7 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_2566 -4.2 -5.8 Chromosome (plasmid) partitioning protein ParA conserved
Pf6N2E2_2754 -4.2 -4.8 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf6N2E2_2707 -4.2 -4.1 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_2626 -4.1 -9.6 Flagellar L-ring protein FlgH compare
Pf6N2E2_5733 -4.1 -5.7 NLP/P60 family protein compare
Pf6N2E2_2506 -4.1 -9.5 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2570 -4.0 -5.2 Signal transduction histidine kinase CheA (EC 2.7.3.-) conserved
Pf6N2E2_2565 -4.0 -10.1 CheW domain protein conserved
Pf6N2E2_3753 -4.0 -15.3 DamX, an inner membrane protein involved in bile resistance compare
Pf6N2E2_5128 -4.0 -4.8 COG1496: Uncharacterized conserved protein compare
Pf6N2E2_5694 -4.0 -6.1 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_2568 -4.0 -6.1 Flagellar motor rotation protein MotA conserved
Pf6N2E2_5160 -4.0 -1.9 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5338 -3.9 -7.5 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_3655 -3.9 -11.9 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_3677 -3.9 -11.1 Sensory box/GGDEF family protein compare
Pf6N2E2_5014 -3.9 -1.3 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_5430 -3.9 -9.1 Transglycosylase, Slt family compare
Pf6N2E2_2518 -3.9 -2.7 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2577 -3.9 -7.9 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_1625 -3.9 -3.8 Ferric reductase (1.6.99.14) compare
Pf6N2E2_2602 -3.9 -7.3 Flagellin protein FlaA compare
Pf6N2E2_5693 -3.8 -11.0 Chemotaxis protein methyltransferase CheR (EC 2.1.1.80) conserved
Pf6N2E2_2599 -3.8 -6.9 Flagellar biosynthesis protein FliS conserved
Pf6N2E2_2513 -3.8 -3.7 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5340 -3.8 -2.6 Stringent starvation protein A compare
Pf6N2E2_445 -3.8 -5.8 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_2753 -3.7 -2.6 HtrA protease/chaperone protein compare
Pf6N2E2_4353 -3.7 -4.9 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_4084 -3.6 -7.3 Transcriptional regulator compare
Pf6N2E2_2564 -3.6 -5.1 Positive regulator of CheA protein activity (CheW) conserved
Pf6N2E2_2514 -3.6 -3.5 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_4416 -3.6 -2.5 Tyrosine recombinase XerC compare
Pf6N2E2_4764 -3.5 -5.9 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_2591 -3.5 -4.4 Flagellar assembly protein FliH compare
Pf6N2E2_4678 -3.5 -1.2 hypothetical protein compare
Pf6N2E2_69 -3.5 -7.4 Probable type IV pilus assembly FimV-related transmembrane protein conserved
Pf6N2E2_2189 -3.4 -4.7 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_1147 -3.4 -3.0 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_2824 -3.4 -7.6 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_3661 -3.4 -2.3 hypothetical protein compare
Pf6N2E2_2509 -3.4 -1.5 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2572 -3.4 -2.8 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_4743 -3.3 -1.1 hypothetical protein compare
Pf6N2E2_2574 -3.3 -2.2 Flagellar synthesis regulator FleN compare
Pf6N2E2_4585 -3.3 -11.7 Cell division protein FtsX compare
Pf6N2E2_4611 -3.3 -3.2 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_5339 -3.3 -6.3 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_2510 -3.3 -3.2 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_2303 -3.3 -7.0 Aerotaxis receptor Aer conserved
Pf6N2E2_5527 -3.3 -2.2 Cold shock protein CspC compare
Pf6N2E2_4187 -3.2 -3.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_5705 -3.2 -3.0 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_2180 -3.2 -3.1 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_2507 -3.2 -4.9 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_5001 -3.2 -2.2 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_5242 -3.2 -2.2 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_5316 -3.2 -2.2 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_2825 -3.1 -2.2 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_5689 -3.1 -3.3 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_5129 -3.1 -11.5 ClpB protein conserved
Pf6N2E2_2311 -3.1 -7.4 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf6N2E2_5677 -3.1 -1.5 Carbon storage regulator compare
Pf6N2E2_1886 -3.1 -2.3 hypothetical protein compare
Pf6N2E2_4631 -3.0 -7.2 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_4153 -3.0 -1.7 Sensory box histidine kinase compare
Pf6N2E2_2314 -3.0 -2.0 VapB protein (antitoxin to VapC) compare
Pf6N2E2_2578 -3.0 -1.8 Flagellar biosynthesis protein FliR compare
Pf6N2E2_156 -2.9 -2.7 Carbon storage regulator compare
Pf6N2E2_3761 -2.9 -13.6 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_2589 -2.9 -3.6 Flagellar protein FliJ compare
Pf6N2E2_3786 -2.9 -3.9 Twin-arginine translocation protein TatC compare
Pf6N2E2_4057 -2.9 -1.0 lipoprotein, putative compare
Pf6N2E2_2552 -2.8 -6.0 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_5291 -2.8 -15.8 Fumarylacetoacetase (EC 3.7.1.2) compare
Pf6N2E2_3713 -2.8 -2.2 Acyl carrier protein compare
Pf6N2E2_3656 -2.8 -5.3 Probable transcription regulator Mig-14 compare
Pf6N2E2_5679 -2.8 -5.0 hypothetical protein compare
Pf6N2E2_4204 -2.8 -1.8 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_3257 -2.7 -2.7 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_5331 -2.7 -6.1 ATPase, AFG1 family compare
Pf6N2E2_4279 -2.7 -8.4 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_5508 -2.7 -1.8 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_3875 -2.7 -1.2 FIG00953324: hypothetical protein compare
Pf6N2E2_2586 -2.6 -1.7 Hpt domain protein compare
Pf6N2E2_6064 -2.6 -5.0 2-methylaconitate isomerase compare
Pf6N2E2_5893 -2.6 -6.7 FIG00956448: hypothetical protein conserved
Pf6N2E2_3607 -2.6 -8.0 3'-to-5' exoribonuclease RNase R compare
Pf6N2E2_4759 -2.6 -11.1 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_5223 -2.5 -3.6 Molybdopterin biosynthesis protein MoeB compare
Pf6N2E2_5520 -2.5 -5.8 Sensory box histidine kinase compare
Pf6N2E2_5178 -2.5 -1.7 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_2152 -2.5 -1.7 hypothetical protein compare
Pf6N2E2_4499 -2.5 -2.3 protein of unknown function DUF86 compare
Pf6N2E2_4352 -2.5 -2.9 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_621 -2.5 -6.7 TPR domain protein in aerotolerance operon compare
Pf6N2E2_5156 -2.4 -1.7 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4763 -2.4 -6.4 Biotin synthesis protein BioC compare
Pf6N2E2_5068 -2.4 -9.2 putative Cytochrome bd2, subunit I compare
Pf6N2E2_3460 -2.4 -1.6 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_4889 -2.4 -6.5 Putative transport protein compare
Pf6N2E2_1870 -2.4 -2.5 hypothetical protein compare
Pf6N2E2_3667 -2.4 -1.3 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_2310 -2.4 -8.5 Cytochrome c oxidase subunit CcoN (EC 1.9.3.1) compare
Pf6N2E2_2313 -2.4 -3.6 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_3027 -2.4 -1.4 Baseplate assembly protein V compare
Pf6N2E2_5069 -2.4 -4.0 putative Cytochrome bd2, subunit II compare
Pf6N2E2_423 -2.4 -1.2 Putative phosphatase YfbT compare
Pf6N2E2_2181 -2.4 -1.9 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_4417 -2.3 -3.0 Protein of unknown function DUF484 compare
Pf6N2E2_4372 -2.3 -4.5 FIG00460773: hypothetical protein compare
Pf6N2E2_6057 -2.3 -2.5 FIG002781: Alpha-L-glutamate ligase family protein compare
Pf6N2E2_3844 -2.3 -2.3 hypothetical protein compare
Pf6N2E2_6062 -2.3 -5.5 2-methylcitrate synthase (EC 2.3.3.5) (from data) compare
Pf6N2E2_3984 -2.3 -7.4 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_1352 -2.3 -2.8 Cation transport ATPase compare
Pf6N2E2_5956 -2.3 -5.9 FIG004453: protein YceG like compare
Pf6N2E2_5944 -2.3 -5.7 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
Pf6N2E2_5184 -2.3 -9.5 Chromosome segregation ATPases conserved
Pf6N2E2_4996 -2.3 -2.5 LSU m3Psi1915 methyltransferase RlmH compare
Pf6N2E2_4160 -2.3 -1.1 Cytochrome c5 compare
Pf6N2E2_2201 -2.3 -3.8 Transmembrane regulator protein PrtR compare
Pf6N2E2_3151 -2.3 -3.1 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_6063 -2.2 -6.8 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_2281 -2.2 -2.1 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_5153 -2.2 -5.7 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_2634 -2.2 -8.0 Potassium uptake protein TrkH compare
Pf6N2E2_2763 -2.2 -8.3 Predicted signal transduction protein conserved
Pf6N2E2_2106 -2.2 -1.4 hypothetical protein compare
Pf6N2E2_2755 -2.2 -1.4 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_3039 -2.2 -4.1 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_582 -2.2 -7.8 Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1) compare
Pf6N2E2_2176 -2.2 -2.2 Amidases related to nicotinamidase compare
Pf6N2E2_2752 -2.2 -6.9 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_1656 -2.1 -1.7 hypothetical protein compare
Pf6N2E2_5313 -2.1 -4.6 Chemotaxis protein CheV (EC 2.7.3.-) compare
Pf6N2E2_3349 -2.1 -2.9 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_4168 -2.1 -5.5 Zinc ABC transporter, inner membrane permease protein ZnuB compare
Pf6N2E2_4803 -2.1 -1.5 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_4206 -2.1 -1.2 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4636 -2.1 -4.5 periplasmic binding protein, putative compare
Pf6N2E2_2316 -2.1 -8.3 Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation compare
Pf6N2E2_4761 -2.1 -6.6 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_4710 -2.1 -1.1 hypothetical protein compare
Pf6N2E2_5186 -2.1 -6.3 FIG00954491: hypothetical protein conserved
Pf6N2E2_624 -2.1 -4.2 hypothetical protein PA3071 compare
Pf6N2E2_2503 -2.1 -1.3 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_2795 -2.1 -1.4 putative exported protein compare


Specific Phenotypes

For 59 genes in this experiment

For motility Agar in Pseudomonas fluorescens FW300-N2E2

For motility Agar across organisms