Experiment set3IT088 for Pseudomonas fluorescens FW300-N2E2

Compare to:

inner cut, LB soft agar motility assay

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5720 +9.9 53.8 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_1981 +9.1 10.9 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_2022 +9.0 23.2 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_3625 +7.5 34.7 Flagellar motor rotation protein MotA compare
Pf6N2E2_2756 +7.4 24.0 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_2217 +6.8 32.9 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_3624 +6.4 18.4 Flagellar motor rotation protein MotB compare
Pf6N2E2_2438 +6.3 4.9 hypothetical protein compare
Pf6N2E2_3827 +5.5 6.7 FIG00955429: hypothetical protein conserved
Pf6N2E2_4351 +4.8 24.2 Predicted signal transduction protein compare
Pf6N2E2_5084 +4.7 22.7 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_3508 +4.6 27.0 Probable two-component response regulator conserved
Pf6N2E2_5874 +4.2 29.6 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf6N2E2_2218 +3.8 12.4 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_4075 +3.8 10.1 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_3060 +3.8 9.1 Outer membrane protein H precursor compare
Pf6N2E2_4615 +3.7 5.8 type IV pili signal transduction protein PilI compare
Pf6N2E2_3062 +3.6 9.0 Membrane-associated zinc metalloprotease compare
Pf6N2E2_2178 +3.5 6.7 FIG00960144: hypothetical protein compare
Pf6N2E2_2406 +3.3 17.4 Periplasmic binding protein compare
Pf6N2E2_5692 +3.3 7.2 Chemotaxis protein CheV (EC 2.7.3.-) conserved
Pf6N2E2_4618 +3.2 7.2 Chemotaxis signal transduction protein compare
Pf6N2E2_5873 +3.1 10.1 FIG00953467: hypothetical protein compare
Pf6N2E2_5872 +3.1 9.0 Sensor histidine kinase/response regulator compare
Pf6N2E2_813 +2.9 7.6 Response regulator compare
Pf6N2E2_4616 +2.8 11.8 type IV pilus biogenesis protein PilJ conserved
Pf6N2E2_4414 +2.8 4.4 FIG00953808: hypothetical protein compare
Pf6N2E2_2601 +2.7 5.5 Flagellin protein FlaG conserved
Pf6N2E2_4152 +2.7 11.2 alginate biosynthesis transcriptional regulatory protein AlgB compare
Pf6N2E2_815 +2.7 14.4 Signal transduction histidine kinase compare
Pf6N2E2_1674 +2.6 10.2 Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) compare
Pf6N2E2_1676 +2.6 5.2 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_4150 +2.5 10.1 probable exported protein YPO0432 compare
Pf6N2E2_3871 +2.5 16.7 FIG00553872: hypothetical protein compare
Pf6N2E2_1675 +2.5 12.5 Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16) compare
Pf6N2E2_4072 +2.3 5.2 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_2329 +2.3 11.6 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) conserved
Pf6N2E2_818 +2.3 8.6 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_2302 +2.2 7.5 CAAX amino terminal protease family protein compare
Pf6N2E2_2195 +2.2 2.6 cI compare
Pf6N2E2_4617 +2.2 12.3 Signal transduction histidine kinase CheA (EC 2.7.3.-) conserved
Pf6N2E2_3830 +2.1 6.8 Glutaredoxin 3 (Grx3) compare
Pf6N2E2_3829 +2.1 5.9 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf6N2E2_4614 +2.1 4.8 twitching motility protein PilH compare
Pf6N2E2_2973 +2.1 4.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_1729 +2.0 3.1 FIG00464907: hypothetical protein compare
Pf6N2E2_446 +2.0 10.3 Glutathione reductase (EC 1.8.1.7) compare
Pf6N2E2_3626 +2.0 4.3 Predicted signal transduction protein compare
Pf6N2E2_2472 +1.9 4.1 hypothetical protein compare
Pf6N2E2_3868 +1.9 8.7 Transcriptional regulator, LysR family compare
Pf6N2E2_4612 +1.9 5.6 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_816 +1.9 10.6 Chemotaxis protein methyltransferase CheR (EC 2.1.1.80) compare
Pf6N2E2_2640 +1.9 1.4 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_2474 +1.9 9.8 ErfK/YbiS/YcfS/YnhG family protein compare
Pf6N2E2_4074 +1.8 12.6 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_3504 +1.8 10.9 Biosynthetic arginine decarboxylase (EC 4.1.1.19) compare
Pf6N2E2_1677 +1.8 4.3 Chalcone synthase (EC 2.3.1.74) compare
Pf6N2E2_3610 +1.7 4.6 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_2857 +1.7 2.3 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) compare
Pf6N2E2_4926 +1.7 11.0 hypothetical protein compare
Pf6N2E2_3876 +1.6 4.7 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_817 +1.6 5.4 Protein-glutamate methylesterase (EC 3.1.1.61) compare
Pf6N2E2_5255 +1.6 10.4 Outer membrane stress sensor protease DegS compare
Pf6N2E2_5219 +1.6 7.2 FIG140336: TPR domain protein compare
Pf6N2E2_2473 +1.6 2.2 Major outer membrane lipoprotein I compare
Pf6N2E2_546 +1.6 1.3 FIG00966904: hypothetical protein compare
Pf6N2E2_3727 +1.6 1.4 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.60) compare
Pf6N2E2_3835 +1.5 3.4 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_5278 +1.5 8.1 Signal transduction histidine kinase compare
Pf6N2E2_2175 +1.5 3.4 Transcriptional regulator, RpiR family compare
Pf6N2E2_3480 +1.5 2.7 Transcriptional regulator, ArsR family compare
Pf6N2E2_3942 +1.5 6.4 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_2912 +1.5 1.5 FIG00954094: hypothetical protein compare
Pf6N2E2_2441 +1.4 4.1 putative ATP/GTP-binding protein compare
Pf6N2E2_2587 +1.4 8.7 Serine phosphatase RsbU, regulator of sigma subunit conserved
Pf6N2E2_3351 +1.4 4.3 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_814 +1.4 3.6 hypothetical protein compare
Pf6N2E2_4362 +1.4 3.2 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_3096 +1.4 1.8 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_4721 +1.4 2.3 Sarcosine oxidase gamma subunit (EC 1.5.3.1) compare
Pf6N2E2_2081 +1.4 1.8 putative exported protein compare
Pf6N2E2_1678 +1.3 6.7 Major facilitator family transporter compare
Pf6N2E2_2282 +1.3 4.5 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf6N2E2_5987 +1.3 5.2 OmpA family protein compare
Pf6N2E2_4669 +1.3 3.8 ElaA protein compare
Pf6N2E2_2674 +1.3 1.5 FIG00953856: hypothetical protein compare
Pf6N2E2_5714 +1.2 2.0 Flavoprotein WrbA compare
Pf6N2E2_2471 +1.2 6.6 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_5785 +1.2 2.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2531 +1.2 4.1 Lipid A export ATP-binding/permease protein MsbA compare
Pf6N2E2_5919 +1.2 1.9 Flp pilus assembly protein TadD, contains TPR repeats compare
Pf6N2E2_3074 +1.2 2.1 FIG00960973: hypothetical protein compare
Pf6N2E2_1761 +1.2 2.3 Transcriptional regulator, LysR family compare
Pf6N2E2_2502 +1.2 3.4 O-antigen acetylase compare
Pf6N2E2_5950 +1.2 3.3 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_2179 +1.2 1.6 Putrescine importer compare
Pf6N2E2_4422 +1.2 2.4 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_4453 +1.1 8.5 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_4401 +1.1 4.6 Glycine cleavage system transcriptional activator compare
Pf6N2E2_5632 +1.1 3.1 Aromatic amino acid transport protein AroP compare
Pf6N2E2_5059 +1.1 6.0 Transcriptional regulator, TetR family compare
Pf6N2E2_3037 +1.1 6.0 RNA polymerase sigma factor RpoS compare
Pf6N2E2_5103 +1.1 1.8 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_342 +1.1 1.4 DNA-binding response regulator compare
Pf6N2E2_5567 +1.1 1.5 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) compare
Pf6N2E2_1778 +1.1 2.5 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_2621 +1.1 6.5 glycosyl transferase, group 2 family protein compare
Pf6N2E2_762 +1.1 5.8 FIG00960186: hypothetical protein compare
Pf6N2E2_1012 +1.1 2.3 hypothetical protein compare
Pf6N2E2_1730 +1.1 3.4 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_4029 +1.0 1.7 Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-) compare
Pf6N2E2_3505 +1.0 1.7 Translation initiation factor SUI1-related protein compare
Pf6N2E2_510 +1.0 6.3 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_2699 +1.0 5.5 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_969 +1.0 2.4 Putative uncharacterized protein STY4534 (Putative uncharacterized protein) compare
Pf6N2E2_2262 +1.0 1.1 Phosphonoacetaldehyde phosphonohydrolase-related protein compare
Pf6N2E2_3134 +1.0 0.9 hypothetical protein compare
Pf6N2E2_5491 +1.0 6.3 FOG: GGDEF domain conserved
Pf6N2E2_3845 +1.0 3.3 A/G-specific adenine glycosylase (EC 3.2.2.-) compare
Pf6N2E2_595 +1.0 2.3 Arsenical resistance operon repressor compare
Pf6N2E2_1044 +1.0 2.2 hypothetical protein compare
Pf6N2E2_4551 +1.0 2.7 hypothetical protein compare
Pf6N2E2_295 +1.0 1.5 Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6) compare
Pf6N2E2_2469 +1.0 3.6 hypothetical protein compare
Pf6N2E2_4337 +1.0 5.4 Uncharacterized protein ImpI/VasC compare
Pf6N2E2_2964 +1.0 2.5 FIG006285: ICC-like protein phosphoesterase compare
Pf6N2E2_4489 +1.0 2.5 Putative activity regulator of membrane protease YbbK compare
Pf6N2E2_4010 +1.0 3.2 Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1) compare
Pf6N2E2_5986 +1.0 1.4 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5943 +0.9 0.7 hypothetical protein compare
Pf6N2E2_2750 +0.9 4.9 Putative permease PerM (= YfgO) compare
Pf6N2E2_5936 +0.9 2.0 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_5704 +0.9 3.3 Transcriptional regulatory protein PhoP compare
Pf6N2E2_379 +0.9 3.5 hypothetical protein compare
Pf6N2E2_3338 +0.9 1.7 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_4155 +0.9 4.8 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_1036 +0.9 3.1 Biopolymer transport protein ExbD/TolR compare
Pf6N2E2_3597 +0.9 2.0 hypothetical protein compare
Pf6N2E2_6140 +0.9 0.9 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_3509 +0.9 2.5 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_4928 +0.9 1.3 Permeases of the major facilitator superfamily compare
Pf6N2E2_855 +0.9 2.0 Transcriptional regulator, IclR family compare
Pf6N2E2_1781 +0.9 3.0 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_4421 +0.9 2.2 hypothetical protein compare
Pf6N2E2_4415 +0.9 1.5 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_5591 +0.9 3.3 hypothetical protein compare
Pf6N2E2_541 +0.9 3.2 Transcriptional regulator, Cro/CI family compare
Pf6N2E2_2612 +0.9 5.7 O-antigen biosynthesis protein compare
Pf6N2E2_1980 +0.9 1.2 hypothetical protein compare
Pf6N2E2_3837 +0.9 4.9 ABC-type amino acid transport, signal transduction systems, periplasmic component/domain compare
Pf6N2E2_1602 +0.9 1.7 hypothetical protein compare
Pf6N2E2_4994 +0.9 2.0 Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) compare
Pf6N2E2_2467 +0.8 1.9 5'-nucleotidase (EC 3.1.3.5) compare
Pf6N2E2_3833 +0.8 2.6 hypothetical protein compare
Pf6N2E2_3346 +0.8 2.9 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_5754 +0.8 4.9 Long-chain fatty acid transport protein compare
Pf6N2E2_1839 +0.8 2.0 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-) compare
Pf6N2E2_4591 +0.8 2.9 tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) compare
Pf6N2E2_775 +0.8 1.8 FIG00955061: hypothetical protein compare
Pf6N2E2_2286 +0.8 1.4 FIG00953287: hypothetical protein compare
Pf6N2E2_5865 +0.8 4.8 Chaperone protein HtpG compare
Pf6N2E2_442 +0.8 1.3 FIG00962345: hypothetical protein compare
Pf6N2E2_763 +0.8 2.3 Sigma-54 dependent transcriptional regulator compare
Pf6N2E2_4459 +0.8 0.9 Transcriptional regulator, GntR family compare
Pf6N2E2_3828 +0.8 1.4 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_1510 +0.8 2.0 FIG00450095: hypothetical protein compare
Pf6N2E2_240 +0.8 2.3 L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1) compare
Pf6N2E2_1476 +0.8 2.1 (AF179595) Vco33 compare
Pf6N2E2_4546 +0.8 1.9 hypothetical protein compare
Pf6N2E2_5052 +0.8 1.4 Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family compare
Pf6N2E2_1743 +0.8 1.4 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) compare
Pf6N2E2_3270 +0.8 1.5 Hypothetical ATP-binding protein UPF0042, contains P-loop compare
Pf6N2E2_2023 +0.8 2.7 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) compare
Pf6N2E2_3200 +0.8 2.9 Putative membrane protein compare
Pf6N2E2_405 +0.8 1.1 hypothetical protein compare
Pf6N2E2_6031 +0.8 2.3 hypothetical protein compare
Pf6N2E2_5647 +0.8 2.8 Transcriptional regulator, TetR family compare
Pf6N2E2_1037 +0.8 2.4 hypothetical protein compare
Pf6N2E2_2630 +0.8 1.7 Phenylalanine 4-monooxygenase (EC 1.14.16.1) (from data) compare
Pf6N2E2_4392 +0.8 1.3 Translation initiation inhibitor compare
Pf6N2E2_4817 +0.8 5.8 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_3077 +0.8 1.6 FIG138056: a glutathione-dependent thiol reductase compare
Pf6N2E2_1932 +0.8 3.1 Transcriptional regulator, IclR family compare
Pf6N2E2_2435 +0.8 2.9 Deoxyguanosinetriphosphate triphosphohydrolase (EC 3.1.5.1) compare
Pf6N2E2_6047 +0.8 1.5 hypothetical protein compare
Pf6N2E2_5097 +0.8 1.9 FIG00953595: hypothetical protein compare
Pf6N2E2_4836 +0.8 3.0 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) compare
Pf6N2E2_463 +0.8 2.5 Transcriptional regulatory protein RstA compare
Pf6N2E2_2961 +0.8 2.9 Succinylglutamate desuccinylase/aspartoacylase compare
Pf6N2E2_525 +0.7 3.5 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_1488 +0.7 2.6 hypothetical protein compare
Pf6N2E2_2407 +0.7 1.1 Acyclic terpenes utilization regulator AtuR, TetR family compare
Pf6N2E2_58 +0.7 1.1 tRNA-dihydrouridine synthase C (EC 1.-.-.-) compare
Pf6N2E2_396 +0.7 2.2 hypothetical protein compare
Pf6N2E2_2480 +0.7 0.9 FIG00957636: hypothetical protein compare
Pf6N2E2_5235 +0.7 3.4 Uracil phosphoribosyltransferase (EC 2.4.2.9) compare
Pf6N2E2_3627 +0.7 3.8 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) compare
Pf6N2E2_4708 +0.7 1.2 hypothetical protein compare
Pf6N2E2_374 +0.7 1.6 FIG00953249: hypothetical protein compare
Pf6N2E2_5230 +0.7 4.4 COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA compare


Specific Phenotypes

For 59 genes in this experiment

For motility Agar in Pseudomonas fluorescens FW300-N2E2

For motility Agar across organisms