Experiment set3IT085 for Paraburkholderia bryophila 376MFSha3.1

LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

Group: supernatant
Media: LB (0.5x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.5 x)
Culturing: Burk376_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 5/2/18
Media components: 5 g/L Tryptone, 2.5 g/L Yeast Extract, 2.5 g/L Sodium Chloride (final concentrations)
Growth plate: Plate4b C1

Specific Phenotypes

For 6 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Paraburkholderia bryophila 376MFSha3.1

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	2
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Glycerophospholipid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cardiolipin biosynthesis II	3	3	1
cardiolipin biosynthesis III	3	2	1
cardiolipin biosynthesis I	3	2	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
phosphatidylglycerol biosynthesis II	6	6	1
phosphatidylglycerol biosynthesis I	6	6	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
superpathway of phospholipid biosynthesis III (E. coli)	12	10	1
superpathway of cardiolipin biosynthesis (bacteria)	13	9	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
superpathway of phospholipid biosynthesis II (plants)	28	10	1