Experiment set3IT083 for Pseudomonas fluorescens FW300-N2E2

LB with copper (II) chloride dihydrate 3 mM

Group: stress
Media: LB + copper (II) chloride dihydrate (3 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.2 generations
By: Mark on 3/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: plate8 A3

Specific Phenotypes

For 16 genes in this experiment

For stress copper (II) chloride dihydrate in Pseudomonas fluorescens FW300-N2E2

For stress copper (II) chloride dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Copper homeostasis	4
Lysine degradation	2
Potassium homeostasis	1
Protein degradation	1
Serine-glyoxylate cycle	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway
• Galactose metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Streptomycin biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucose biosynthesis	2	2	1
GDP-α-D-glucose biosynthesis	3	2	1
trehalose degradation V	3	2	1
starch degradation V	4	3	1
glycogen biosynthesis I (from ADP-D-Glucose)	4	3	1
sucrose degradation IV (sucrose phosphorylase)	4	3	1
starch degradation III	4	2	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
glucose and glucose-1-phosphate degradation	5	4	1
sucrose degradation II (sucrose synthase)	5	4	1
CDP-6-deoxy-D-gulose biosynthesis	5	3	1
D-galactose degradation I (Leloir pathway)	5	3	1
glucosylglycerol biosynthesis	5	2	1
glycogen degradation II	6	5	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
glycogen degradation I	8	6	1
sucrose biosynthesis II	8	6	1
glycogen biosynthesis III (from α-maltose 1-phosphate)	8	3	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
chitin biosynthesis	9	6	1
starch biosynthesis	10	5	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
colanic acid building blocks biosynthesis	11	9	1
streptomycin biosynthesis	18	3	1
superpathway of anaerobic sucrose degradation	19	14	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1