Experiment set3IT082 for Shewanella sp. ANA-3

LB with Spectinomycin dihydrochloride pentahydrate 0.025 mg/ml

Group: stress
Media: LB + Spectinomycin dihydrochloride pentahydrate (0.025 mg/ml)
Culturing: ANA3_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/15/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 928 C7,C8

Specific Phenotypes

For 8 genes in this experiment

For stress Spectinomycin dihydrochloride pentahydrate in Shewanella sp. ANA-3

For stress Spectinomycin dihydrochloride pentahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Biogenesis of c-type cytochromes	1
Biogenesis of cbb3-type cytochrome c oxidases	1
Copper homeostasis	1
Terminal cytochrome C oxidases	1
Terminal cytochrome O ubiquinol oxidase	1
Terminal cytochrome oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Methionine metabolism
• Tryptophan metabolism
• Riboflavin metabolism
• Porphyrin and chlorophyll metabolism
• Terpenoid biosynthesis
• Carotenoid biosynthesis - General
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
heme a biosynthesis	4	2	1