Experiment set3IT081 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with L-Lysine 25 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_1676 +1.2 4.0 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_4305 +1.1 1.6 FIG00959590: hypothetical protein compare
Pf6N2E2_2156 +1.1 2.9 Putative polyketide synthase (Fragment) compare
Pf6N2E2_3325 +1.0 3.9 ABC transporter, ATP-binding protein compare
Pf6N2E2_2967 +1.0 2.8 Cold shock protein CspA compare
Pf6N2E2_5766 +1.0 0.9 Quaternary ammonium compound-resistance protein SugE compare
Pf6N2E2_4148 +0.9 6.5 Glutamate-aspartate carrier protein compare
Pf6N2E2_288 +0.9 2.2 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_5219 +0.9 6.9 FIG140336: TPR domain protein compare
Pf6N2E2_2106 +0.9 1.5 hypothetical protein compare
Pf6N2E2_4414 +0.9 2.6 FIG00953808: hypothetical protein compare
Pf6N2E2_2519 +0.9 2.2 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_6099 +0.9 2.1 tRNA-Glu-TTC compare
Pf6N2E2_3835 +0.9 3.1 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_98 +0.9 2.1 putative transmembrane protein compare
Pf6N2E2_2973 +0.8 4.0 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5340 +0.8 3.5 Stringent starvation protein A compare
Pf6N2E2_5757 +0.8 2.9 hypothetical protein compare
Pf6N2E2_4601 +0.8 2.5 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_6045 +0.8 2.0 hypothetical protein compare
Pf6N2E2_1438 +0.8 4.0 Methyltransferase (EC 2.1.1.-) compare
Pf6N2E2_1040 +0.8 2.3 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_5972 +0.8 2.7 hypothetical protein compare
Pf6N2E2_5237 +0.8 2.8 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_3625 +0.7 5.7 Flagellar motor rotation protein MotA compare
Pf6N2E2_962 +0.7 2.4 Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) compare
Pf6N2E2_2022 +0.7 4.7 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_2281 +0.7 2.6 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_5111 +0.7 2.0 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_4635 +0.7 4.8 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_5720 +0.7 5.9 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_5546 +0.7 4.1 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_2375 +0.7 2.1 Response regulator NasT compare
Pf6N2E2_5320 +0.7 4.7 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf6N2E2_3338 +0.7 1.8 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_3199 +0.7 4.0 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_2402 +0.7 1.8 hypothetical protein compare
Pf6N2E2_5987 +0.6 3.8 OmpA family protein compare
Pf6N2E2_3240 +0.6 1.5 Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) compare
Pf6N2E2_3033 +0.6 0.8 hypothetical protein compare
Pf6N2E2_5207 +0.6 2.0 hypothetical protein compare
Pf6N2E2_490 +0.6 2.0 PhaK-like protein compare
Pf6N2E2_3969 +0.6 0.8 Membrane-fusion protein compare
Pf6N2E2_4708 +0.6 1.9 hypothetical protein compare
Pf6N2E2_1113 +0.6 1.9 Transcriptional regulator, TetR family compare
Pf6N2E2_5545 +0.6 3.0 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_1325 +0.6 2.0 FIG00954773: hypothetical protein compare
Pf6N2E2_3624 +0.6 3.8 Flagellar motor rotation protein MotB compare
Pf6N2E2_2080 +0.6 1.3 hypothetical protein compare
Pf6N2E2_1032 +0.6 1.4 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_6059 +0.6 2.1 FIG008443: hypothetical protein compare
Pf6N2E2_4640 +0.6 1.7 hypothetical protein compare
Pf6N2E2_3956 +0.6 3.1 FIG00960943: hypothetical protein compare
Pf6N2E2_3634 +0.6 0.6 Transporter compare
Pf6N2E2_659 +0.6 1.7 Transcriptional regulatory protein ompR compare
Pf6N2E2_4057 +0.6 0.8 lipoprotein, putative compare
Pf6N2E2_2373 +0.6 1.9 Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid) compare
Pf6N2E2_6022 +0.6 2.4 StbE replicon stabilization toxin compare
Pf6N2E2_3514 +0.6 2.1 Ribosomal protein L11 methyltransferase (EC 2.1.1.-) compare
Pf6N2E2_2502 +0.6 2.5 O-antigen acetylase compare
Pf6N2E2_504 +0.6 1.4 DNA-binding response regulator compare
Pf6N2E2_2546 +0.6 2.2 Carbon storage regulator compare
Pf6N2E2_2594 +0.6 3.3 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_3849 +0.6 2.2 FIG00954739: hypothetical protein compare
Pf6N2E2_5209 +0.6 2.8 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_2013 +0.6 1.1 hypothetical protein compare
Pf6N2E2_4052 +0.6 1.4 FIG00955481: hypothetical protein compare
Pf6N2E2_1192 +0.6 2.4 Transcriptional regulator compare
Pf6N2E2_4351 +0.6 4.6 Predicted signal transduction protein compare
Pf6N2E2_2444 +0.6 3.0 Glutathione peroxidase family protein compare
Pf6N2E2_6058 +0.6 3.4 FIG139976: hypothetical protein compare
Pf6N2E2_579 +0.6 0.9 FIG00962753: hypothetical protein compare
Pf6N2E2_3973 +0.6 2.6 hypothetical protein compare
Pf6N2E2_1195 +0.6 2.1 hypothetical protein compare
Pf6N2E2_37 +0.6 1.7 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_4365 +0.6 3.7 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_2602 +0.5 3.9 Flagellin protein FlaA compare
Pf6N2E2_1249 +0.5 1.3 Transcriptional regulator, AraC family compare
Pf6N2E2_2413 +0.5 1.3 FIG074102: hypothetical protein compare
Pf6N2E2_2795 +0.5 1.6 putative exported protein compare
Pf6N2E2_1299 +0.5 1.8 Transcriptional regulator, TetR family compare
Pf6N2E2_1981 +0.5 2.0 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_2425 +0.5 1.8 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_4540 +0.5 1.4 hypothetical protein compare
Pf6N2E2_2 +0.5 1.9 FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis compare
Pf6N2E2_6093 +0.5 2.1 hypothetical protein compare
Pf6N2E2_3019 +0.5 3.4 FIG00954559: hypothetical protein compare
Pf6N2E2_5865 +0.5 4.2 Chaperone protein HtpG compare
Pf6N2E2_5088 +0.5 2.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_180 +0.5 2.6 Transcriptional regulator, MerR family compare
Pf6N2E2_3274 +0.5 3.1 Manganese superoxide dismutase (EC 1.15.1.1) compare
Pf6N2E2_4624 +0.5 0.9 Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-) compare
Pf6N2E2_2088 +0.5 1.3 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf6N2E2_4810 +0.5 3.2 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_5086 +0.5 1.0 hypothetical protein compare
Pf6N2E2_2582 +0.5 1.7 Flagellar motor switch protein FliN compare
Pf6N2E2_5989 +0.5 4.3 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_2109 +0.5 2.8 NAD-dependent protein deacetylase of SIR2 family compare
Pf6N2E2_6046 +0.5 2.6 hypothetical protein compare
Pf6N2E2_251 +0.5 2.5 transcriptional regulator compare
Pf6N2E2_3597 +0.5 1.8 hypothetical protein compare
Pf6N2E2_4422 +0.5 2.0 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_282 +0.5 1.3 GNAT family acetyltransferase YjcF compare
Pf6N2E2_1463 +0.5 1.1 Isochorismate pyruvate-lyase (EC 4.-.-.-) compare
Pf6N2E2_1754 +0.5 2.5 FIG00731982: hypothetical protein compare
Pf6N2E2_2622 +0.5 4.0 Flagellar hook-associated protein FlgL compare
Pf6N2E2_1343 +0.5 2.5 Transcriptional regulator compare
Pf6N2E2_5695 +0.5 3.3 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_723 +0.5 1.9 FIG138517: Putative lipid carrier protein compare
Pf6N2E2_1968 +0.5 1.2 Cobalt-zinc-cadmium resistance protein compare
Pf6N2E2_4499 +0.5 0.9 protein of unknown function DUF86 compare
Pf6N2E2_1886 +0.5 1.0 hypothetical protein compare
Pf6N2E2_5679 +0.5 1.7 hypothetical protein compare
Pf6N2E2_1701 +0.5 2.3 ABC transporter ATP-binding protein compare
Pf6N2E2_5318 +0.5 4.3 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf6N2E2_1814 +0.5 2.1 Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases compare
Pf6N2E2_4809 +0.5 3.9 COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases compare
Pf6N2E2_3619 +0.5 3.2 NAD(P)HX epimerase / NAD(P)HX dehydratase compare
Pf6N2E2_1404 +0.5 2.1 Nitrous oxide reductase maturation transmembrane protein NosY compare
Pf6N2E2_5611 +0.5 2.6 SAM-dependent methyltransferases compare
Pf6N2E2_3762 +0.5 2.6 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_2526 +0.5 2.1 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_2600 +0.5 3.6 Flagellar hook-associated protein FliD compare
Pf6N2E2_3429 +0.5 1.0 hypothetical protein compare
Pf6N2E2_5293 +0.5 2.0 Protein sirB1 compare
Pf6N2E2_2518 +0.5 1.1 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_4508 +0.5 2.3 Transcriptional regulator, TetR family compare
Pf6N2E2_2971 +0.4 3.9 AmpG permease compare
Pf6N2E2_2436 +0.4 2.4 DNA-binding response regulator, LuxR family compare
Pf6N2E2_5719 +0.4 2.2 D-lactate dehydrogenase (EC 1.1.1.28) compare
Pf6N2E2_5694 +0.4 2.8 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_2071 +0.4 1.6 hypothetical protein compare
Pf6N2E2_5943 +0.4 0.8 hypothetical protein compare
Pf6N2E2_848 +0.4 2.2 hypothetical protein compare
Pf6N2E2_5567 +0.4 0.9 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) compare
Pf6N2E2_5133 +0.4 1.8 FIG00953980: hypothetical protein compare
Pf6N2E2_4364 +0.4 0.8 Protein YicC compare
Pf6N2E2_220 +0.4 1.9 Sensory histidine kinase BaeS compare
Pf6N2E2_5623 +0.4 1.3 YciL protein compare
Pf6N2E2_2620 +0.4 2.5 O-antigen biosynthesis protein compare
Pf6N2E2_5084 +0.4 3.3 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_3386 +0.4 1.8 hypothetical protein compare
Pf6N2E2_5236 +0.4 1.3 hypothetical protein compare
Pf6N2E2_1534 +0.4 1.4 Probable transmembrane protein compare
Pf6N2E2_5411 +0.4 1.6 FIG00955494: hypothetical protein compare
Pf6N2E2_2714 +0.4 1.4 transcriptional regulator, Crp/Fnr family compare
Pf6N2E2_2536 +0.4 0.9 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_4838 +0.4 3.1 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
Pf6N2E2_5575 +0.4 2.4 Glycerol uptake facilitator protein compare
Pf6N2E2_1440 +0.4 1.4 Putative tRNA-m1A22 methylase compare
Pf6N2E2_4963 +0.4 1.1 Homoserine dehydrogenase (EC 1.1.1.3) compare
Pf6N2E2_868 +0.4 1.2 Transcriptional regulator, HxlR family compare
Pf6N2E2_1213 +0.4 1.6 hypothetical protein compare
Pf6N2E2_5321 +0.4 2.9 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf6N2E2_4706 +0.4 1.2 Electron transfer flavoprotein, beta subunit compare
Pf6N2E2_663 +0.4 2.1 Transcriptional activator of acetoin dehydrogenase operon AcoR compare
Pf6N2E2_5279 +0.4 2.0 Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C) compare
Pf6N2E2_1665 +0.4 2.0 hypothetical protein compare
Pf6N2E2_5955 +0.4 1.8 Aminodeoxychorismate lyase (EC 4.1.3.38) compare
Pf6N2E2_1774 +0.4 1.0 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_3138 +0.4 1.8 YaeQ protein compare
Pf6N2E2_2501 +0.4 1.2 hypothetical protein compare
Pf6N2E2_4074 +0.4 3.6 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_2573 +0.4 2.4 RNA polymerase sigma factor for flagellar operon compare
Pf6N2E2_3105 +0.4 1.9 hypothetical protein compare
Pf6N2E2_1781 +0.4 2.0 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_434 +0.4 1.0 hypothetical protein compare
Pf6N2E2_2583 +0.4 2.5 Flagellar motor switch protein FliM compare
Pf6N2E2_2693 +0.4 0.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_3271 +0.4 1.1 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3206 +0.4 0.9 hypothetical protein compare
Pf6N2E2_2101 +0.4 2.1 hypothetical protein compare
Pf6N2E2_2196 +0.4 1.7 FIG00953347: hypothetical protein compare
Pf6N2E2_1317 +0.4 1.8 P-hydroxyphenylacetate hydroxylase C2:oxygenase component compare
Pf6N2E2_5030 +0.4 2.0 Transcriptional regulator, AsnC family compare
Pf6N2E2_402 +0.4 2.1 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_6087 +0.4 1.6 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_5714 +0.4 1.0 Flavoprotein WrbA compare
Pf6N2E2_5741 +0.4 2.0 L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81) compare
Pf6N2E2_5974 +0.4 2.2 Uncharacterized glutathione S-transferase-like protein compare
Pf6N2E2_2626 +0.4 2.8 Flagellar L-ring protein FlgH compare
Pf6N2E2_4770 +0.4 2.0 Stress induced hydrophobic peptide compare
Pf6N2E2_1911 +0.4 2.1 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_3942 +0.4 2.8 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_3516 +0.4 0.8 Probable transmembrane protein compare
Pf6N2E2_1548 +0.4 1.3 Lactoylglutathione lyase compare
Pf6N2E2_5689 +0.4 1.8 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_786 +0.4 1.9 Type-1 fimbrial protein, A chain precursor compare
Pf6N2E2_5344 +0.4 2.0 Predicted endonuclease distantly related to archaeal Holliday junction resolvase compare
Pf6N2E2_2045 +0.4 1.5 Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8) compare
Pf6N2E2_2549 +0.4 0.9 hypothetical protein compare
Pf6N2E2_4721 +0.4 1.4 Sarcosine oxidase gamma subunit (EC 1.5.3.1) compare
Pf6N2E2_3870 +0.4 0.9 hypothetical protein compare
Pf6N2E2_3344 +0.4 1.5 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD compare
Pf6N2E2_4113 +0.4 1.4 Redox-sensing transcriptional regulator QorR compare
Pf6N2E2_699 +0.4 1.7 Heme d1 biosynthesis protein NirG compare
Pf6N2E2_2599 +0.4 2.1 Flagellar biosynthesis protein FliS compare
Pf6N2E2_1656 +0.4 0.9 hypothetical protein compare
Pf6N2E2_3738 +0.4 1.7 General secretion pathway protein L compare
Pf6N2E2_4451 +0.4 2.3 FIG139438: lipoprotein B compare


Specific Phenotypes

For 7 genes in this experiment

For stress L-Lysine in Pseudomonas fluorescens FW300-N2E2

For stress L-Lysine across organisms