Experiment set3IT080 for Pseudomonas fluorescens FW300-N2E2

LB with Choline Lysine 20 mM

Group: stress
Media: LB + Choline Lysine (20 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.3 generations
By: Mark on 3/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: plate7 D2

Specific Phenotypes

For 24 genes in this experiment

For stress Choline Lysine in Pseudomonas fluorescens FW300-N2E2

For stress Choline Lysine across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter oligopeptide (TC 3.A.1.5.1)	1
Aromatic amino acid degradation	1
Chorismate Synthesis	1
Cobalt-zinc-cadmium resistance	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
Control of cell elongation - division cycle in Bacilli	1
Cysteine Biosynthesis	1
DNA Repair Base Excision	1
DNA repair, UvrABC system	1
Glycine and Serine Utilization	1
Peptidoglycan Biosynthesis	1
Pyridoxin (Vitamin B6) Biosynthesis	1
Serine Biosynthesis	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Glycine, serine and threonine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Glutathione metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
3-dehydroquinate biosynthesis I	2	2	1
L-serine biosynthesis I	3	3	1
L-cysteine biosynthesis IX (Trichomonas vaginalis)	3	2	1
superpathway of L-serine and glycine biosynthesis I	4	4	1
chorismate biosynthesis I	7	7	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of L-tryptophan biosynthesis	13	13	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of chorismate metabolism	59	43	1