Experiment set3IT080 for Pseudomonas fluorescens FW300-N2E3

LB with Tetracycline hydrochloride 0.0008 mg/ml

Group: stress
Media: LB + Tetracycline hydrochloride (8e-04 mg/ml)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 6/26/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 956 B7,B8

Specific Phenotypes

For 2 genes in this experiment

For stress Tetracycline hydrochloride in Pseudomonas fluorescens FW300-N2E3

For stress Tetracycline hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tryptophan metabolism
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Methane metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation IV	3	3	1
reactive oxygen species degradation	4	4	1