Experiment set3IT079 for Pseudomonas fluorescens SBW25-INTG

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Growth at pH8 and carbon source Trisodium citrate dihydrate

Group: pH
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + Trisodium citrate dihydrate (10 mM) + Ammonium chloride (10 mM) + TAPS sodium salt (40 mM), pH=8
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 15 genes in this experiment

For pH Trisodium citrate dihydrate in Pseudomonas fluorescens SBW25-INTG

For pH Trisodium citrate dihydrate across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 1
Experimental tye 1
Glutamate dehydrogenases 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Heme and Siroheme Biosynthesis 1
High affinity phosphate transporter and control of PHO regulon 1
Methionine Biosynthesis 1
Phosphate metabolism 1
Proline Synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis III 1 1 1
5-(methoxycarbonylmethoxy)uridine biosynthesis 3 1 1
heme b biosynthesis II (oxygen-independent) 4 3 1
superpathway of heme b biosynthesis from uroporphyrinogen-III 6 4 1
L-glutamate and L-glutamine biosynthesis 7 6 1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) 9 3 1
superpathway of bacteriochlorophyll a biosynthesis 26 5 1