Experiment set3IT079 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Paraquat dichloride 0.1 mg/ml

Group: stress
Media: BHIS + Paraquat dichloride (0.1 mg/ml)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: Btheta_sp2_copyB C2

Specific Phenotypes

For 5 genes in this experiment

For stress Paraquat dichloride in Bacteroides thetaiotaomicron VPI-5482

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem	#Specific
Glycine cleavage system	1
Leucine Degradation and HMG-CoA Metabolism	1
Photorespiration (oxidative C2 cycle)	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
TCA Cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Biosynthesis of alkaloids derived from shikimate pathway
• Glycolysis / Gluconeogenesis
• Citrate cycle (TCA cycle)
• Ubiquinone and menaquinone biosynthesis
• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine degradation
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Pyruvate metabolism
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine cleavage	3	3	1
glycine biosynthesis II	3	3	1
2-oxoglutarate decarboxylation to succinyl-CoA	3	2	1
pyruvate decarboxylation to acetyl CoA I	3	2	1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA	3	2	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	17	1