Experiment set3IT078 for Pseudomonas fluorescens FW300-N2E3

Compare to:

LB with Chloramphenicol 0.0025 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
AO353_17305 +1.3 6.3 TetR family transcriptional regulator compare
AO353_07105 +1.1 2.1 biotin--protein ligase compare
AO353_01485 +1.1 6.8 ABC transporter compare
AO353_25925 +1.1 2.7 hypothetical protein compare
AO353_13100 +1.1 2.6 GTPase RsgA compare
AO353_00090 +1.0 2.8 hypothetical protein compare
AO353_06490 +1.0 3.5 nicotinate-nucleotide adenylyltransferase compare
AO353_24915 +0.9 2.0 hypothetical protein compare
AO353_08620 +0.9 2.7 hypothetical protein compare
AO353_01595 +0.9 2.6 Flp pilus assembly protein CpaB compare
AO353_28555 +0.9 2.2 LysR family transcriptional regulator compare
AO353_03115 +0.9 3.3 TetR family transcriptional regulator compare
AO353_24535 +0.9 2.1 hypothetical protein compare
AO353_05625 +0.9 1.4 transcription elongation factor GreA compare
AO353_01480 +0.9 6.0 hypothetical protein compare
AO353_26830 +0.8 3.7 histidine kinase compare
AO353_21455 +0.8 2.2 cation:proton antiporter compare
AO353_16040 +0.8 2.4 hypothetical protein compare
AO353_04410 +0.8 2.8 phosphoribosylglycinamide formyltransferase compare
AO353_04065 +0.8 2.5 electron transporter RnfB compare
AO353_17885 +0.8 2.0 hypothetical protein compare
AO353_12765 +0.8 2.8 transcriptional regulator compare
AO353_13515 +0.8 3.5 Urease (EC 3.5.1.5) (from data) compare
AO353_27585 +0.7 1.8 DNA repair protein compare
AO353_27720 +0.7 2.7 cupin compare
AO353_13420 +0.7 1.0 hypothetical protein compare
AO353_02970 +0.7 2.3 carbon storage regulator compare
AO353_14120 +0.7 3.2 hypothetical protein compare
AO353_16925 +0.7 6.1 AmpG family muropeptide MFS transporter compare
AO353_28945 +0.7 1.3 potassium-transporting ATPase subunit B compare
AO353_00125 +0.7 1.9 hypothetical protein compare
AO353_00260 +0.7 1.9 hypothetical protein compare
AO353_00020 +0.7 3.5 delta-aminolevulinic acid dehydratase compare
AO353_16665 +0.7 3.1 phage tail protein compare
AO353_13610 +0.7 2.1 precorrin isomerase compare
AO353_04220 +0.7 3.6 chemotaxis protein CheY compare
AO353_12690 +0.7 1.0 acyl carrier protein compare
AO353_07140 +0.7 5.7 peptidase M23 compare
AO353_25955 +0.7 2.2 NIPSNAP family containing protein compare
AO353_12345 +0.6 1.2 twin-arginine protein translocation system subunit TatC compare
AO353_22985 +0.6 2.0 hypothetical protein compare
AO353_21465 +0.6 1.5 cation:proton antiporter compare
AO353_17205 +0.6 1.8 beta-ketoadipyl CoA thiolase compare
AO353_29355 +0.6 2.4 hypothetical protein compare
AO353_12225 +0.6 2.6 hypothetical protein compare
AO353_26670 +0.6 2.8 ArsR family transcriptional regulator compare
AO353_00120 +0.6 1.5 DNA-binding protein compare
AO353_02015 +0.6 3.5 acetyltransferase compare
AO353_12880 +0.6 0.9 ADP-heptose--LPS heptosyltransferase compare
AO353_09815 +0.6 3.4 hypothetical protein compare
AO353_02815 +0.6 3.3 transcriptional regulator compare
AO353_00480 +0.6 2.7 hypothetical protein compare
AO353_15000 +0.6 2.3 glycerate dehydrogenase compare
AO353_21750 +0.6 1.7 peptidylprolyl isomerase compare
AO353_02935 +0.6 2.9 ribose-phosphate pyrophosphokinase compare
AO353_02945 +0.6 3.3 magnesium transporter compare
AO353_09400 +0.6 2.4 hypothetical protein compare
AO353_03935 +0.6 2.2 fructose-bisphosphate aldolase compare
AO353_16930 +0.6 2.0 DNA base-flipping protein YbaZ compare
AO353_07145 +0.6 4.3 anhydro-N-acetylmuramic acid kinase compare
AO353_23440 +0.6 1.5 hypothetical protein compare
AO353_02660 +0.6 1.8 peptidase P60 compare
AO353_00955 +0.6 1.4 cytotoxic translational repressor of toxin-antitoxin stability system compare
AO353_27575 +0.6 1.6 hypothetical protein compare
AO353_28080 +0.6 2.1 hypothetical protein compare
AO353_24120 +0.6 1.1 hypothetical protein compare
AO353_00455 +0.6 2.1 beta-hexosaminidase compare
AO353_22560 +0.5 2.1 hypothetical protein compare
AO353_21560 +0.5 2.5 type VI secretion protein compare
AO353_22195 +0.5 1.3 metal ABC transporter permease compare
AO353_22930 +0.5 1.5 hypothetical protein compare
AO353_03515 +0.5 1.2 cold-shock protein compare
AO353_05195 +0.5 3.8 glmZ(sRNA)-inactivating NTPase compare
AO353_08265 +0.5 2.8 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD compare
AO353_22280 +0.5 1.4 hypothetical protein compare
AO353_27825 +0.5 1.9 peptidylprolyl isomerase compare
AO353_01045 +0.5 1.2 phasin compare
AO353_24990 +0.5 2.0 translation elongation factor compare
AO353_04055 +0.5 1.0 methionine--tRNA ligase compare
AO353_08060 +0.5 1.8 hypothetical protein compare
AO353_02600 +0.5 1.4 alpha-ribazole phosphatase compare
AO353_22395 +0.5 2.3 pyrroloquinoline quinone biosynthesis protein PqqB compare
AO353_20790 +0.5 1.9 penicillin-binding protein activator LpoB compare
AO353_22980 +0.5 2.4 hypothetical protein compare
AO353_28860 +0.5 2.0 hypothetical protein compare
AO353_04965 +0.5 2.2 D-erythro-7,8-dihydroneopterin triphosphate epimerase compare
AO353_18790 +0.5 2.0 histidine kinase compare
AO353_09135 +0.5 1.3 cytochrome C compare
AO353_19600 +0.5 2.6 NAD-dependent DNA ligase compare
AO353_18690 +0.5 2.2 flagellar assembly protein FliH compare
AO353_16020 +0.5 2.6 tRNA (cmo5U34)-methyltransferase compare
AO353_26595 +0.5 2.0 copper resistance protein CopD compare
AO353_24740 +0.5 1.3 hypothetical protein compare
AO353_09115 +0.5 2.7 diguanylate cyclase compare
AO353_22110 +0.5 2.0 nitrate reductase compare
AO353_21895 +0.5 1.4 molybdenum cofactor biosynthesis protein MoaD compare
AO353_25160 +0.5 1.9 ABC transporter substrate-binding protein compare
AO353_24760 +0.5 1.6 glutamine amidotransferase compare
AO353_01135 +0.5 1.4 enoyl-CoA hydratase compare
AO353_05675 +0.5 2.7 protein RnfH compare
AO353_20585 +0.5 1.4 TetR family transcriptional regulator compare
AO353_26285 +0.5 1.0 hypothetical protein compare
AO353_13575 +0.5 1.7 hypothetical protein compare
AO353_09495 +0.5 2.3 hypothetical protein compare
AO353_27380 +0.5 1.1 pyocin R2, holin compare
AO353_14030 +0.5 3.8 ABC transporter ATP-binding protein compare
AO353_12790 +0.5 1.1 hypothetical protein compare
AO353_16355 +0.5 1.7 TetR family transcriptional regulator compare
AO353_20225 +0.5 2.7 1-aminocyclopropane-1-carboxylate deaminase compare
AO353_23870 +0.5 2.0 DNA-binding protein compare
AO353_28100 +0.5 2.3 MFS transporter compare
AO353_00255 +0.5 1.9 hypothetical protein compare
AO353_00440 +0.5 2.2 hypothetical protein compare
AO353_10010 +0.5 3.2 homocysteine methyltransferase compare
AO353_11060 +0.5 1.5 holliday junction resolvasome, helicase subunit compare
AO353_02520 +0.5 0.8 sodium transporter compare
AO353_07295 +0.5 3.9 aminoglycoside phosphotransferase compare
AO353_24780 +0.5 2.6 glutamine synthetase compare
AO353_17915 +0.5 2.3 hypothetical protein compare
AO353_28440 +0.5 2.3 alpha/beta hydrolase compare
AO353_02515 +0.5 2.7 recombination-associated protein RdgC compare
AO353_27500 +0.5 1.0 hypothetical protein compare
AO353_23280 +0.5 1.9 hypothetical protein compare
AO353_24255 +0.5 1.5 hypothetical protein compare
AO353_20415 +0.5 1.9 hypothetical protein compare
AO353_10620 +0.4 2.1 hypothetical protein compare
AO353_13870 +0.4 2.5 acyl dehydratase compare
AO353_10085 +0.4 1.6 pyridoxamine kinase compare
AO353_02650 +0.4 2.0 SAM-dependent methyltransferase compare
AO353_27350 +0.4 1.1 hypothetical protein compare
AO353_26450 +0.4 1.8 hypothetical protein compare
AO353_15610 +0.4 1.6 chemotaxis protein CheY compare
AO353_01435 +0.4 1.7 protein tyrosine phosphatase compare
AO353_09345 +0.4 1.3 hypothetical protein compare
AO353_01540 +0.4 2.4 ABC transporter substrate-binding protein compare
AO353_15785 +0.4 2.9 S-adenosylmethionine:tRNA ribosyltransferase-isomerase compare
AO353_27950 +0.4 0.7 hypothetical protein compare
AO353_22595 +0.4 1.6 hypothetical protein compare
AO353_04970 +0.4 1.5 cell division protein DedD compare
AO353_12030 +0.4 1.9 hypothetical protein compare
AO353_07290 +0.4 2.7 mannose-1-phosphate guanylyltransferase compare
AO353_00755 +0.4 2.3 hypothetical protein compare
AO353_24985 +0.4 1.4 L-seryl-tRNA(Sec) selenium transferase compare
AO353_01705 +0.4 1.4 alpha/beta hydrolase compare
AO353_13965 +0.4 1.6 large-conductance mechanosensitive channel compare
AO353_20745 +0.4 2.2 hypothetical protein compare
AO353_23710 +0.4 2.1 protein BatD compare
AO353_17160 +0.4 1.1 aspartate-semialdehyde dehydrogenase compare
AO353_00700 +0.4 2.5 Cro/Cl family transcriptional regulator compare
AO353_20760 +0.4 1.9 hypothetical protein compare
AO353_02655 +0.4 2.5 peptidase P60 compare
AO353_11720 +0.4 1.2 taurine transporter ATP-binding subunit compare
AO353_20810 +0.4 1.1 hypothetical protein compare
AO353_19515 +0.4 1.7 haloacid dehalogenase compare
AO353_23635 +0.4 1.8 hypothetical protein compare
AO353_03125 +0.4 2.3 LysR family transcriptional regulator compare
AO353_09775 +0.4 1.0 hypothetical protein compare
AO353_29160 +0.4 1.5 GTP pyrophosphokinase compare
AO353_27930 +0.4 1.8 LysR family transcriptional regulator compare
AO353_26415 +0.4 1.3 hypothetical protein compare
AO353_23385 +0.4 2.6 trans-2-enoyl-CoA reductase compare
AO353_28455 +0.4 2.0 FMN-dependent NADH-azoreductase compare
AO353_12625 +0.4 1.7 hypothetical protein compare
AO353_03915 +0.4 2.6 LysR family transcriptional regulator compare
AO353_24655 +0.4 1.8 hypothetical protein compare
AO353_16795 +0.4 1.3 lysozyme compare
AO353_22510 +0.4 1.3 hypothetical protein compare
AO353_05795 +0.4 2.3 GNAT family acetyltransferase compare
AO353_24435 +0.4 2.6 flavoprotein compare
AO353_05240 +0.4 1.1 rod shape-determining protein MreD compare
AO353_04415 +0.4 1.7 preQ0 transporter compare
AO353_25845 +0.4 1.1 GNAT family acetyltransferase compare
AO353_09265 +0.4 1.1 hypothetical protein compare
AO353_24260 +0.4 0.8 hypothetical protein compare
AO353_18455 +0.4 2.1 AraC family transcriptional regulator compare
AO353_00870 +0.4 1.3 hypothetical protein compare
AO353_18990 +0.4 2.5 transcription elongation factor GreB compare
AO353_25490 +0.4 1.3 transcriptional regulator compare
AO353_13590 +0.4 2.0 cobalt-precorrin-6X reductase compare
AO353_19685 +0.4 1.5 alkylhydroperoxidase compare
AO353_20000 +0.4 1.5 phosphoribosyltransferase compare
AO353_22955 +0.4 1.1 tRNA hydroxylase compare
AO353_08880 +0.4 1.1 exopolyphosphatase compare
AO353_03135 +0.4 1.5 hypothetical protein compare
AO353_23170 +0.4 1.4 short-chain dehydrogenase compare
AO353_07645 +0.4 0.8 sarcosine oxidase subunit gamma compare
AO353_25680 +0.4 1.6 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (from data) compare
AO353_00100 +0.4 1.6 hypothetical protein compare
AO353_11940 +0.4 1.1 hypothetical protein compare
AO353_04830 +0.4 2.0 required for acetate efflux, together with NodT, MFP and FUSC proteins (AO353_04840, AO353_04825, and AO353_04835) (from data) compare
AO353_12560 +0.4 1.8 type II secretion system protein GspG compare
AO353_02465 +0.4 0.6 hypothetical protein compare
AO353_22145 +0.4 2.2 FTR1 family iron permease compare
AO353_21775 +0.4 1.4 transporter compare
AO353_07270 +0.4 1.2 transcriptional regulator compare
AO353_05435 +0.4 2.4 choline ABC transporter permease compare
AO353_04275 +0.4 2.0 polyamine ABC transporter ATP-binding protein compare
AO353_19090 +0.4 0.8 MarR family transcriptional regulator compare
AO353_19825 +0.4 2.5 shikimate dehydrogenase compare
AO353_20040 +0.4 1.6 hypothetical protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E3 in stress experiments

For stress Chloramphenicol across organisms