Experiment set3IT078 for Bacteroides thetaiotaomicron VPI-5482

BHIS with sodium fluoride 30 mM

Group: stress
Media: BHIS + sodium fluoride (30 mM)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 11-Oct-17
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Growth plate: Btheta_sp2_copyB B6

Specific Phenotypes

For 5 genes in this experiment

For stress sodium fluoride in Bacteroides thetaiotaomicron VPI-5482

For stress sodium fluoride across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	1
Potassium homeostasis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
sulfate activation for sulfonation	2	2	1
assimilatory sulfate reduction II	3	1	1
sulfite oxidation III	3	1	1
assimilatory sulfate reduction III	3	1	1
assimilatory sulfate reduction IV	4	2	1
assimilatory sulfate reduction I	4	2	1
selenate reduction	5	2	1
dissimilatory sulfate reduction I (to hydrogen sufide))	5	1	1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens)	6	1	1
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans)	6	1	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	7	1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	4	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	8	1
superpathway of sulfide oxidation (phototrophic sulfur bacteria)	12	2	1
superpathway of seleno-compound metabolism	19	5	1