Experiment set3IT076 for Acidovorax sp. GW101-3H11

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LB with Cobalt chloride hexahydrate 0.32 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.32 mM)
Culturing: acidovorax_3H11_ML3a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.3 generations
By: Mark on 05/19/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 13 genes in this experiment

For stress Cobalt chloride hexahydrate in Acidovorax sp. GW101-3H11

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Transport of Iron 2
Acetyl-CoA fermentation to Butyrate 1
Butanol Biosynthesis 1
Cobalt-zinc-cadmium resistance 1
Glycine and Serine Utilization 1
Heat shock dnaK gene cluster extended 1
Heme and Siroheme Biosynthesis 1
Iron acquisition in Vibrio 1
Isoleucine degradation 1
L-rhamnose utilization 1
Lactate utilization 1
Polyhydroxybutyrate metabolism 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Queuosine-Archaeosine Biosynthesis 1
Respiratory dehydrogenases 1 1
Serine Biosynthesis 1
Valine degradation 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
benzoyl-CoA biosynthesis 3 3 2
fatty acid salvage 6 5 3
linoleate biosynthesis II (animals) 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
oleate β-oxidation (thioesterase-dependent, yeast) 2 1 1
oleate β-oxidation 35 29 17
fatty acid β-oxidation I (generic) 7 5 3
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 2
glutaryl-CoA degradation 5 3 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
pyruvate fermentation to hexanol (engineered) 11 7 4
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 6 4
2-methyl-branched fatty acid β-oxidation 14 10 5
L-serine biosynthesis I 3 3 1
pyruvate fermentation to butanol II (engineered) 6 5 2
propanoate fermentation to 2-methylbutanoate 6 5 2
L-isoleucine degradation I 6 5 2
valproate β-oxidation 9 7 3
methylglyoxal degradation V 3 2 1
L-cysteine biosynthesis IX (Trichomonas vaginalis) 3 2 1
3-methyl-branched fatty acid α-oxidation 6 3 2
6-gingerol analog biosynthesis (engineered) 6 3 2
methyl ketone biosynthesis (engineered) 6 3 2
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
L-glutamate degradation V (via hydroxyglutarate) 10 6 3
benzoyl-CoA degradation I (aerobic) 7 6 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
pyruvate fermentation to butanoate 7 3 2
superpathway of L-serine and glycine biosynthesis I 4 4 1
L-valine degradation I 8 6 2
phytol degradation 4 3 1
pyruvate fermentation to butanol I 8 4 2
long chain fatty acid ester synthesis (engineered) 4 2 1
phosphatidylcholine acyl editing 4 2 1
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
wax esters biosynthesis II 4 1 1
phenylacetate degradation I (aerobic) 9 5 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 4 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 2
sporopollenin precursors biosynthesis 18 4 4
4-hydroxybenzoate biosynthesis III (plants) 5 4 1
3-phenylpropanoate degradation 10 6 2
octane oxidation 5 3 1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 1 1
superpathway of phenylethylamine degradation 11 6 2
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
methylthiopropanoate degradation I (cleavage) 6 2 1
L-glutamate degradation VII (to butanoate) 12 3 2
stearate biosynthesis I (animals) 6 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 2
superpathway of glyoxylate cycle and fatty acid degradation 14 11 2
L-glutamate degradation XI (reductive Stickland reaction) 7 3 1
ceramide degradation by α-oxidation 7 2 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
capsaicin biosynthesis 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
Spodoptera littoralis pheromone biosynthesis 22 4 3
L-tryptophan degradation III (eukaryotic) 15 7 2
glycerol degradation to butanol 16 10 2
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 4 2
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
superpathway of dimethylsulfoniopropanoate degradation 8 2 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 2 1
2-methylpropene degradation 8 2 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 2
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 5 2
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 2
photorespiration I 9 4 1
toluene degradation VI (anaerobic) 18 4 2
methyl tert-butyl ether degradation 10 2 1
suberin monomers biosynthesis 20 3 2
superpathway of fatty acid biosynthesis II (plant) 43 38 4
gallate degradation III (anaerobic) 11 4 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
androstenedione degradation I (aerobic) 25 6 2
platensimycin biosynthesis 26 7 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
1-butanol autotrophic biosynthesis (engineered) 27 19 2
androstenedione degradation II (anaerobic) 27 4 2
superpathway of testosterone and androsterone degradation 28 6 2
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 3
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 8 3
cutin biosynthesis 16 1 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 2 1
superpathway of cholesterol degradation III (oxidase) 49 4 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 17 1
superpathway of fatty acids biosynthesis (E. coli) 53 49 2
palmitate biosynthesis III 29 28 1