Experiment set3IT074 for Rhodanobacter denitrificans FW104-10B01
R2A with SpecU metal mix 1 X
Group: stressMedia: R2A + 1X SpecU
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
SpecU 1x includes: 0.008 mM Cobalt chloride hexahydrate, 0.002 mM Cadmium chloride hemipentahydrate, 0.053 mM Nickel (II) chloride hexahydrate, 2.25 mM Aluminum chloride hydrate, 1 mM Manganese (II) chloride tetrahydrate, 0.01 mM Zinc chloride, 0.0045 mM Uranyl acetate
Specific Phenotypes
For 15 genes in this experiment
For stress SpecU in Rhodanobacter denitrificans FW104-10B01
For stress SpecU across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalt-zinc-cadmium resistance | 1 |
| Mannose Metabolism | 1 |
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| guanine and guanosine salvage II | 2 | 2 | 1 |
| xanthine and xanthosine salvage | 2 | 2 | 1 |
| guanine and guanosine salvage I | 2 | 2 | 1 |
| superpathway of guanine and guanosine salvage | 3 | 2 | 1 |
| adenine salvage | 3 | 2 | 1 |
| adenine and adenosine salvage III | 4 | 3 | 1 |
| superpathway of purine nucleotide salvage | 14 | 14 | 1 |