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Experiment set3IT074 for
Methanococcus maripaludis S2
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS08860
+1.1
6.7
MinD/ParA family protein
compare
MMP
_RS08855
+1.1
8.4
hypothetical protein
compare
MMP
_RS08675
+1.0
5.9
class III signal peptide-containing protein
compare
MMP
_RS00240
+0.9
7.6
type II secretion system F family protein
compare
MMP
_RS08580
+0.8
6.2
flagellin
compare
MMP
_RS00245
+0.8
7.6
type II secretion system F family protein
compare
MMP
_RS08615
+0.8
2.5
flagellar protein G
compare
MMP
_RS01280
+0.8
6.9
DUF515 domain-containing protein
compare
MMP
_RS01275
+0.8
5.7
class III signal peptide-containing protein
compare
MMP
_RS01290
+0.8
7.4
class III signal peptide-containing protein
compare
MMP
_RS01305
+0.8
7.4
hypothetical protein
compare
MMP
_RS01315
+0.8
7.3
DUF2341 domain-containing protein
compare
MMP
_RS00250
+0.8
6.7
Flp pilus assembly complex ATPase component TadA
compare
MMP
_RS01270
+0.7
6.7
prepilin peptidase
compare
MMP
_RS01285
+0.7
6.4
hypothetical protein
compare
MMP
_RS08940
+0.7
1.9
MetS family NSS transporter small subunit
compare
MMP
_RS01310
+0.7
6.8
hypothetical protein
compare
MMP
_RS07330
+0.7
6.6
membrane protein
compare
MMP
_RS05865
+0.7
2.2
hypothetical protein
compare
MMP
_RS01295
+0.7
5.9
hypothetical protein
compare
MMP
_RS04015
+0.7
2.8
hypothetical protein
compare
MMP
_RS08750
+0.7
2.0
hypothetical protein
compare
MMP
_RS08400
+0.6
2.2
MJ0307 family thioredoxin
compare
MMP
_RS05985
+0.6
2.5
hypothetical protein
compare
MMP
_RS07775
+0.6
2.6
alanine dehydrogenase
compare
MMP
_RS00140
+0.6
3.4
DUF192 domain-containing protein
compare
MMP
_RS08845
+0.6
4.7
archaellum operon transcriptional activator EarA family protein
compare
MMP
_RS01570
+0.6
1.7
hypothetical protein
compare
MMP
_RS07345
+0.6
3.0
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS01085
+0.6
2.0
hypothetical protein
compare
MMP
_RS05635
+0.6
4.3
NAD-dependent epimerase/dehydratase family protein
compare
MMP
_RS09055
+0.6
1.1
CAP domain-containing protein
compare
MMP
_RS03125
+0.5
3.9
cell division ATPase MinD
compare
MMP
_RS08830
+0.5
3.0
GTP-binding protein
compare
MMP
_RS08410
+0.5
2.8
DUF169 domain-containing protein
compare
MMP
_RS08850
+0.5
4.7
hypothetical protein
compare
MMP
_RS08310
+0.5
1.7
TMEM165/GDT1 family protein
compare
MMP
_RS00535
+0.5
2.6
TIGR00375 family protein
compare
MMP
_RS08485
+0.5
3.6
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS07905
+0.4
2.5
DUF3343 domain-containing protein
compare
MMP
_RS06760
+0.4
1.3
hypothetical protein
compare
MMP
_RS00655
+0.4
2.2
hypothetical protein
compare
MMP
_RS01655
+0.4
2.1
DsrE family protein
compare
MMP
_RS07765
+0.4
3.1
sodium:alanine symporter family protein
compare
MMP
_RS07805
+0.4
3.0
ABC transporter permease
compare
MMP
_RS06035
+0.4
3.1
glycosyltransferase family 2 protein
compare
MMP
_RS01790
+0.4
2.3
restriction endonuclease
compare
MMP
_RS07840
+0.4
1.3
ribonuclease III
compare
MMP
_RS08665
+0.4
3.5
hypothetical protein
compare
MMP
_RS02605
+0.4
0.6
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS08080
+0.4
2.2
YchF/TatD family DNA exonuclease
compare
MMP
_RS03550
+0.4
1.5
DUF2124 domain-containing protein
compare
MMP
_RS03535
+0.4
1.5
helix-turn-helix transcriptional regulator
compare
MMP
_RS05785
+0.4
3.4
ATP-binding protein
compare
MMP
_RS08405
+0.4
2.8
MFS transporter
compare
MMP
_RS08770
+0.4
2.6
hypothetical protein
compare
MMP
_RS08700
+0.4
2.8
hypothetical protein
compare
MMP
_RS08640
+0.4
2.8
TIM barrel protein
compare
MMP
_RS03965
+0.4
1.5
hypothetical protein
compare
MMP
_RS07550
+0.4
2.7
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS00570
+0.4
0.8
protein-L-isoaspartate O-methyltransferase
compare
MMP
_RS06935
+0.4
2.2
protease complex subunit PrcB family protein
compare
MMP
_RS00295
+0.4
1.5
gamma carbonic anhydrase family protein
compare
MMP
_RS06140
+0.4
1.9
radical SAM protein
compare
MMP
_RS00395
+0.4
1.1
MGMT family protein
compare
MMP
_RS03080
+0.4
1.0
universal stress protein
compare
MMP
_RS07415
+0.4
2.2
molybdopterin-guanine dinucleotide biosynthesis protein MobB
compare
MMP
_RS08775
+0.4
2.2
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS04205
+0.4
1.9
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS01255
+0.4
2.1
amino acid ABC transporter ATP-binding protein
compare
MMP
_RS07895
+0.3
1.8
cytochrome c
compare
MMP
_RS00345
+0.3
2.2
DUF2666 domain-containing protein
compare
MMP
_RS06575
+0.3
1.8
fluoride efflux transporter CrcB
compare
MMP
_RS01630
+0.3
2.6
N-glycosylase/DNA lyase
compare
MMP
_RS00710
+0.3
1.7
hypothetical protein
compare
MMP
_RS01675
+0.3
1.1
hypothetical protein
compare
MMP
_RS08835
+0.3
2.4
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS03520
+0.3
0.7
YwbE family protein
compare
MMP
_RS07985
+0.3
2.9
nitroreductase family protein
compare
MMP
_RS01685
+0.3
1.6
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS04610
+0.3
2.3
Dabb family protein
compare
MMP
_RS09045
+0.3
2.7
class III signal peptide-containing protein
compare
MMP
_RS08455
+0.3
2.0
multiprotein bridging factor aMBF1
compare
MMP
_RS06175
+0.3
2.4
winged helix-turn-helix transcriptional regulator
compare
MMP
_RS08130
+0.3
2.7
IGHMBP2 family helicase
compare
MMP
_RS08285
+0.3
2.1
serine/threonine protein kinase
compare
MMP
_RS08980
+0.3
1.8
hypothetical protein
compare
MMP
_RS02175
+0.3
0.4
DUF1611 domain-containing protein
compare
MMP
_RS06385
+0.3
1.8
hypothetical protein
compare
MMP
_RS08480
+0.3
2.7
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS07590
+0.3
2.7
hypothetical protein
compare
MMP
_RS08205
+0.3
2.6
stage II sporulation protein M
compare
MMP
_RS08280
+0.3
1.4
proteasome assembly chaperone family protein
compare
MMP
_RS02545
+0.3
1.2
hypothetical protein
compare
MMP
_RS04410
+0.3
1.1
hypothetical protein
compare
MMP
_RS01125
+0.3
2.0
thioredoxin family protein
compare
MMP
_RS08610
+0.3
2.4
flagellar protein F
compare
MMP
_RS08395
+0.3
1.5
DsrE family protein
compare
MMP
_RS08380
+0.3
2.1
phosphatase PAP2 family protein
compare
MMP
_RS07800
+0.3
1.8
ATP-binding cassette domain-containing protein
compare
MMP
_RS01075
+0.3
0.9
hypothetical protein
compare
MMP
_RS05580
+0.3
2.7
glycosyltransferase
compare
MMP
_RS02805
+0.3
0.7
HEPN domain-containing protein
compare
MMP
_RS06540
+0.3
1.3
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS06990
+0.3
1.7
CatB-related O-acetyltransferase
compare
MMP
_RS02295
+0.3
2.3
TatD family hydrolase
compare
MMP
_RS08265
+0.3
1.8
rubrerythrin
compare
MMP
_RS07915
+0.3
1.8
DUF4870 domain-containing protein
compare
MMP
_RS08325
+0.3
2.3
hypothetical protein
compare
MMP
_RS08390
+0.3
1.5
DUF5400 domain-containing protein
compare
MMP
_RS01195
+0.3
0.6
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS09060
+0.3
2.3
hypothetical protein
compare
MMP
_RS05315
+0.3
0.9
hypothetical protein
compare
MMP
_RS00545
+0.3
1.5
XRE family transcriptional regulator
compare
MMP
_RS07715
+0.3
2.0
YfcE family phosphodiesterase
compare
MMP
_RS08235
+0.3
1.8
hypothetical protein
compare
MMP
_RS08090
+0.3
1.5
hypothetical protein
compare
MMP
_RS09065
+0.3
2.3
hypothetical protein
compare
MMP
_RS07640
+0.3
1.8
mechanosensitive ion channel family protein
compare
MMP
_RS07835
+0.3
1.1
TldD/PmbA family protein
compare
MMP
_RS06050
+0.3
1.9
ZIP family metal transporter
compare
MMP
_RS08490
+0.3
2.3
hypothetical protein
compare
MMP
_RS08215
+0.3
1.5
DUF483 domain-containing protein
compare
MMP
_RS07150
+0.3
1.5
MTH895/ArsE family thioredoxin-like protein
compare
MMP
_RS01320
+0.3
1.4
hypothetical protein
compare
MMP
_RS07155
+0.3
1.9
MFS transporter
compare
MMP
_RS06710
+0.3
1.4
selenium-binding protein
compare
MMP
_RS01870
+0.3
1.2
acyl-CoA dehydratase activase
compare
MMP
_RS07890
+0.2
0.7
OB-fold nucleic acid binding domain-containing protein
compare
MMP
_RS06930
+0.2
2.0
MBL fold metallo-hydrolase
compare
MMP
_RS08575
+0.2
2.2
hypothetical protein
compare
MMP
_RS00380
+0.2
1.4
P-II family nitrogen regulator
compare
MMP
_RS06910
+0.2
1.7
RlmF-related methyltransferase
compare
MMP
_RS08250
+0.2
1.6
pyruvate kinase
compare
MMP
_RS02290
+0.2
1.6
MBL fold metallo-hydrolase
compare
MMP
_RS05715
+0.2
1.6
hypothetical protein
compare
MMP
_RS01300
+0.2
2.1
hypothetical protein
compare
MMP
_RS06970
+0.2
1.5
ribosomal protein S18-alanine N-acetyltransferase
compare
MMP
_RS07185
+0.2
1.9
pyridoxal phosphate-dependent aminotransferase
compare
MMP
_RS08260
+0.2
1.7
DUF99 family protein
compare
MMP
_RS01135
+0.2
1.7
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS00155
+0.2
1.8
heavy metal-binding domain-containing protein
compare
MMP
_RS06655
+0.2
1.8
glycoside hydrolase family 15 protein
compare
MMP
_RS03625
+0.2
1.5
cell wall-binding protein
compare
MMP
_RS07595
+0.2
1.9
hypothetical protein
compare
MMP
_RS06965
+0.2
1.7
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS08630
+0.2
1.4
archaellar assembly protein FlaJ
compare
MMP
_RS03545
+0.2
1.8
ATP-binding protein
compare
MMP
_RS06070
+0.2
1.9
iron ABC transporter substrate-binding protein
compare
MMP
_RS04145
+0.2
0.6
hypothetical protein
compare
MMP
_RS00145
+0.2
1.5
ATP-dependent Clp protease proteolytic subunit
compare
MMP
_RS00690
+0.2
1.2
MBL fold metallo-hydrolase
compare
MMP
_RS06560
+0.2
1.7
hypothetical protein
compare
MMP
_RS02260
+0.2
1.6
lactate utilization protein
compare
MMP
_RS05910
+0.2
0.7
cell division ATPase MinD
compare
MMP
_RS00400
+0.2
1.7
tributyrin esterase
compare
MMP
_RS03900
+0.2
1.5
minichromosome maintenance protein MCM
compare
MMP
_RS06325
+0.2
0.5
hypothetical protein
compare
MMP
_RS08230
+0.2
1.3
50S ribosomal protein L11 methyltransferase
compare
MMP
_RS06665
+0.2
1.6
glycogen/starch synthase
compare
MMP
_RS06015
+0.2
0.9
flavodoxin family protein
compare
MMP
_RS06735
+0.2
1.5
class I SAM-dependent rRNA methyltransferase
compare
MMP
_RS07100
+0.2
1.5
hypothetical protein
compare
MMP
_RS05780
+0.2
1.4
multidrug resistance efflux transporter family protein
compare
MMP
_RS00385
+0.2
1.5
P-II family nitrogen regulator
compare
MMP
_RS07085
+0.2
1.3
ribonuclease HII
compare
MMP
_RS01185
+0.2
1.4
hypothetical protein
compare
MMP
_RS00090
+0.2
0.8
CoA-binding protein
compare
MMP
_RS04030
+0.2
1.2
hypothetical protein
compare
MMP
_RS06100
+0.2
1.6
GNAT family N-acetyltransferase
compare
MMP
_RS03230
+0.2
1.2
flavodoxin family protein
compare
MMP
_RS06520
+0.2
1.0
DUF1786 domain-containing protein
compare
MMP
_RS00085
+0.2
1.5
DNA cytosine methyltransferase
compare
MMP
_RS08290
+0.2
1.3
DNA-binding protein Alba
compare
MMP
_RS03530
+0.2
1.5
tetratricopeptide repeat protein
compare
MMP
_RS07910
+0.2
1.0
NAD(P)H-hydrate dehydratase
compare
MMP
_RS08385
+0.2
0.9
ABC transporter substrate-binding protein
compare
MMP
_RS01635
+0.2
1.2
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS03465
+0.2
0.4
adenine phosphoribosyltransferase
compare
MMP
_RS02255
+0.2
1.3
hypothetical protein
compare
MMP
_RS01650
+0.2
0.3
sulfurtransferase TusA family protein
compare
MMP
_RS08620
+0.2
1.3
flagellar accessory protein FlaH
compare
MMP
_RS08655
+0.2
1.6
phosphoadenosine phosphosulfate reductase family protein
compare
MMP
_RS08160
+0.2
0.8
EamA family transporter
compare
MMP
_RS02320
+0.2
1.4
glutamate-cysteine ligase family protein
compare
MMP
_RS01490
+0.2
1.2
tetratricopeptide repeat protein
compare
MMP
_RS05085
+0.2
1.3
AAA family ATPase
compare
MMP
_RS02020
+0.2
1.1
gamma-glutamylcyclotransferase
compare
MMP
_RS07570
+0.2
1.4
nucleotidyltransferase family protein
compare
MMP
_RS06465
+0.2
1.4
NERD domain-containing protein
compare
MMP
_RS01980
+0.2
1.7
trimeric intracellular cation channel family protein
compare
MMP
_RS07855
+0.2
1.5
class I SAM-dependent methyltransferase
compare
MMP
_RS06115
+0.2
1.4
class II aldolase/adducin family protein
compare
MMP
_RS02530
+0.2
1.0
type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family
compare
MMP
_RS07880
+0.2
1.3
AAA family ATPase
compare
MMP
_RS02425
+0.2
1.1
hypothetical protein
compare
MMP
_RS00480
+0.2
1.3
hypothetical protein
compare
MMP
_RS02025
+0.2
1.4
DNA-directed DNA polymerase
compare
MMP
_RS00285
+0.2
1.1
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS01090
+0.2
1.2
ABC transporter substrate-binding protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t1 experiments