Experiment set3IT071 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Nalidixic acid sodium salt 0.008 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_1056 +6.2 8.1 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_5647 +5.8 39.2 Transcriptional regulator, TetR family compare
Pf6N2E2_2822 +5.7 35.8 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_3659 +5.4 19.8 Protein fixF compare
Pf6N2E2_3663 +5.2 39.3 Glycosyl transferase compare
Pf6N2E2_3661 +4.3 20.0 hypothetical protein compare
Pf6N2E2_3908 +3.9 26.4 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2507 +3.4 21.7 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_2513 +3.3 12.3 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_2510 +3.2 18.3 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3658 +3.2 19.9 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_2509 +2.9 11.4 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2506 +2.9 24.7 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2508 +2.8 11.6 hypothetical protein compare
Pf6N2E2_4047 +2.6 15.5 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_3656 +2.5 18.9 Probable transcription regulator Mig-14 compare
Pf6N2E2_4612 +2.5 14.0 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_3349 +2.5 9.7 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_3655 +2.5 20.7 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_4974 +2.4 14.8 DedA protein compare
Pf6N2E2_773 +2.4 17.7 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_3899 +2.4 15.7 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_924 +2.2 3.4 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_5150 +2.1 10.4 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_4077 +2.1 7.5 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_2518 +2.0 3.3 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_3198 +2.0 3.9 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_5525 +1.7 14.5 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases compare
Pf6N2E2_3664 +1.7 7.0 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_926 +1.6 5.5 NADH dehydrogenase (EC 1.6.99.3) compare
Pf6N2E2_3980 +1.6 12.9 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_3791 +1.5 12.4 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_2942 +1.5 11.7 ATP-dependent helicase DinG/Rad3 conserved
Pf6N2E2_3978 +1.5 12.4 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_3979 +1.5 12.4 Type I secretion system ATPase, LssB family LapB compare
Pf6N2E2_3981 +1.5 13.6 T1SS secreted agglutinin RTX compare
Pf6N2E2_4369 +1.5 5.2 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_445 +1.5 8.6 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_5508 +1.5 2.8 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_3792 +1.4 8.6 Glucans biosynthesis protein G precursor compare
Pf6N2E2_3941 +1.4 5.3 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3199 +1.4 8.1 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_6140 +1.4 2.8 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_2264 +1.3 4.6 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf6N2E2_5723 +1.3 10.0 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_2597 +1.3 10.7 Flagellar regulatory protein FleQ compare
Pf6N2E2_2277 +1.3 3.8 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_3624 +1.3 8.0 Flagellar motor rotation protein MotB compare
Pf6N2E2_1298 +1.3 3.9 Xanthine transporter,putative compare
Pf6N2E2_3696 +1.3 2.6 hypothetical protein compare
Pf6N2E2_4654 +1.2 4.1 hypothetical protein compare
Pf6N2E2_5944 +1.2 6.1 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
Pf6N2E2_5720 +1.2 9.0 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_5243 +1.2 3.2 hypothetical protein compare
Pf6N2E2_4601 +1.2 3.4 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_2578 +1.2 3.0 Flagellar biosynthesis protein FliR compare
Pf6N2E2_945 +1.2 1.8 Transcriptional regulator, TetR family compare
Pf6N2E2_2022 +1.2 5.4 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_3625 +1.2 9.5 Flagellar motor rotation protein MotA compare
Pf6N2E2_145 +1.1 6.6 putative exported protein compare
Pf6N2E2_2821 +1.1 3.6 hypothetical protein compare
Pf6N2E2_3983 +1.1 7.6 Membrane bound c-di-GMP receptor LapD compare
Pf6N2E2_586 +1.1 6.4 Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-) compare
Pf6N2E2_144 +1.1 9.6 Uncharacterized protein YtfM precursor compare
Pf6N2E2_2425 +1.1 3.1 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3517 +1.1 5.0 tRNA dihydrouridine synthase B (EC 1.-.-.-) compare
Pf6N2E2_2447 +1.1 5.8 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_3885 +1.1 4.7 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_2514 +1.1 2.3 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_2281 +1.1 2.0 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_2519 +1.0 2.2 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_4584 +1.0 2.3 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_2580 +1.0 4.6 Flagellar biosynthesis protein FliP compare
Pf6N2E2_2593 +1.0 5.6 Flagellar M-ring protein FliF compare
Pf6N2E2_5733 +1.0 5.5 NLP/P60 family protein compare
Pf6N2E2_2278 +1.0 8.3 Transcription-repair coupling factor compare
Pf6N2E2_4579 +1.0 2.8 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_238 +1.0 1.2 hypothetical protein compare
Pf6N2E2_5331 +1.0 5.4 ATPase, AFG1 family compare
Pf6N2E2_3282 +1.0 2.9 FIG005080: Possible exported protein compare
Pf6N2E2_3179 +0.9 1.7 FIG021952: putative membrane protein compare
Pf6N2E2_1691 +0.9 2.1 hypothetical protein compare
Pf6N2E2_2579 +0.9 4.5 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_4969 +0.9 1.7 hypothetical protein compare
Pf6N2E2_1981 +0.9 3.5 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_2582 +0.9 4.0 Flagellar motor switch protein FliN compare
Pf6N2E2_5013 +0.9 3.6 Putative membrane protein compare
Pf6N2E2_4057 +0.9 1.2 lipoprotein, putative compare
Pf6N2E2_4996 +0.9 1.4 LSU m3Psi1915 methyltransferase RlmH compare
Pf6N2E2_4773 +0.9 1.6 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_3762 +0.9 4.4 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_2323 +0.9 1.9 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_4918 +0.9 2.4 Ribonucleotide reductase transcriptional regulator NrdR compare
Pf6N2E2_4048 +0.9 5.1 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_2438 +0.9 2.1 hypothetical protein compare
Pf6N2E2_1215 +0.8 2.6 probable membrane protein YPO2362 compare
Pf6N2E2_2599 +0.8 4.8 Flagellar biosynthesis protein FliS compare
Pf6N2E2_5956 +0.8 3.8 FIG004453: protein YceG like compare
Pf6N2E2_2549 +0.8 1.2 hypothetical protein compare
Pf6N2E2_5697 +0.8 7.0 Flagellar hook protein FlgE compare
Pf6N2E2_2626 +0.8 5.8 Flagellar L-ring protein FlgH compare
Pf6N2E2_2285 +0.8 1.6 FIG00954079: hypothetical protein compare
Pf6N2E2_6045 +0.8 1.7 hypothetical protein compare
Pf6N2E2_1024 +0.8 1.8 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases compare
Pf6N2E2_5520 +0.8 5.1 Sensory box histidine kinase compare
Pf6N2E2_1036 +0.8 3.9 Biopolymer transport protein ExbD/TolR compare
Pf6N2E2_2081 +0.8 1.8 putative exported protein compare
Pf6N2E2_3255 +0.8 2.4 YrbA protein compare
Pf6N2E2_2589 +0.8 3.8 Flagellar protein FliJ compare
Pf6N2E2_5746 +0.8 1.9 Cobalamin synthase compare
Pf6N2E2_5188 +0.8 3.4 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_241 +0.8 1.5 hypothetical protein compare
Pf6N2E2_3271 +0.8 2.3 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_2577 +0.8 4.9 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_1935 +0.8 1.1 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_5689 +0.8 2.8 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_719 +0.8 2.0 Molybdenum cofactor biosynthesis protein MoaB compare
Pf6N2E2_2590 +0.8 5.2 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_1492 +0.8 1.9 No significant database matches compare
Pf6N2E2_2633 +0.8 3.0 Nitroreductase family protein compare
Pf6N2E2_2573 +0.8 4.4 RNA polymerase sigma factor for flagellar operon compare
Pf6N2E2_3641 +0.7 2.1 ADP-ribose pyrophosphatase (EC 3.6.1.13) compare
Pf6N2E2_4025 +0.7 1.0 hypothetical protein compare
Pf6N2E2_3171 +0.7 1.4 hypothetical protein compare
Pf6N2E2_5425 +0.7 1.1 hypothetical protein compare
Pf6N2E2_442 +0.7 3.2 FIG00962345: hypothetical protein compare
Pf6N2E2_37 +0.7 2.9 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_4987 +0.7 1.4 MaoC-like domain protein compare
Pf6N2E2_4351 +0.7 5.4 Predicted signal transduction protein compare
Pf6N2E2_5694 +0.7 3.5 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_3033 +0.7 1.8 hypothetical protein compare
Pf6N2E2_775 +0.7 2.7 FIG00955061: hypothetical protein compare
Pf6N2E2_2602 +0.7 5.0 Flagellin protein FlaA compare
Pf6N2E2_2600 +0.7 5.6 Flagellar hook-associated protein FliD compare
Pf6N2E2_3847 +0.7 2.6 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_2623 +0.7 5.6 Flagellar hook-associated protein FlgK compare
Pf6N2E2_4889 +0.7 4.3 Putative transport protein compare
Pf6N2E2_1611 +0.7 3.6 Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1) compare
Pf6N2E2_5014 +0.7 1.1 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_6090 +0.7 0.8 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_3829 +0.7 2.9 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf6N2E2_5545 +0.7 3.3 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_4477 +0.7 2.2 Z-ring-associated protein ZapA compare
Pf6N2E2_2628 +0.7 2.2 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_2534 +0.7 2.2 Cell division protein BolA compare
Pf6N2E2_5546 +0.7 3.7 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_2138 +0.7 3.2 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) compare
Pf6N2E2_5695 +0.7 5.4 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_1258 +0.7 1.5 FIG00960119: hypothetical protein compare
Pf6N2E2_3870 +0.7 1.1 hypothetical protein compare
Pf6N2E2_987 +0.7 1.1 hypothetical protein compare
Pf6N2E2_97 +0.7 3.2 putataive branched-chain amino acid transport protein AzlC compare
Pf6N2E2_2583 +0.7 3.9 Flagellar motor switch protein FliM compare
Pf6N2E2_358 +0.7 2.5 Haloacid dehalogenase, type II compare
Pf6N2E2_2585 +0.7 4.4 Flagellar hook-length control protein FliK compare
Pf6N2E2_5691 +0.7 3.5 Flagellar basal-body P-ring formation protein FlgA compare
Pf6N2E2_5766 +0.6 0.9 Quaternary ammonium compound-resistance protein SugE compare
Pf6N2E2_282 +0.6 0.8 GNAT family acetyltransferase YjcF compare
Pf6N2E2_546 +0.6 1.2 FIG00966904: hypothetical protein compare
Pf6N2E2_451 +0.6 1.6 transcriptional regulator MvaT, P16 subunit, putative compare
Pf6N2E2_3204 +0.6 0.8 Rho-specific inhibitor of transcription termination (YaeO) compare
Pf6N2E2_2156 +0.6 1.4 Putative polyketide synthase (Fragment) compare
Pf6N2E2_5176 +0.6 4.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_4160 +0.6 1.3 Cytochrome c5 compare
Pf6N2E2_2622 +0.6 4.9 Flagellar hook-associated protein FlgL compare
Pf6N2E2_2488 +0.6 1.6 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3886 +0.6 3.2 ADP compounds hydrolase NudE (EC 3.6.1.-) compare
Pf6N2E2_2315 +0.6 2.7 VapC toxin protein compare
Pf6N2E2_2441 +0.6 3.9 putative ATP/GTP-binding protein compare
Pf6N2E2_5734 +0.6 4.0 NLP/P60 family protein compare
Pf6N2E2_6060 +0.6 2.9 Propionate catabolism operon transcriptional regulator of GntR family [predicted] compare
Pf6N2E2_2625 +0.6 2.0 Flagellar P-ring protein FlgI compare
Pf6N2E2_771 +0.6 2.8 Ribosyl nicotinamide transporter, PnuC-like compare
Pf6N2E2_3029 +0.6 2.6 Holin compare
Pf6N2E2_4993 +0.6 1.2 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) compare
Pf6N2E2_2594 +0.6 3.8 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_4708 +0.6 1.8 hypothetical protein compare
Pf6N2E2_5103 +0.6 1.2 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_440 +0.6 1.6 FIG00954920: hypothetical protein compare
Pf6N2E2_5044 +0.6 1.6 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_2872 +0.6 4.2 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_724 +0.6 1.3 Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family) compare
Pf6N2E2_377 +0.6 1.5 hypothetical protein compare
Pf6N2E2_605 +0.6 2.0 FIG002465: BNR repeat protein compare
Pf6N2E2_2 +0.6 2.2 FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis compare
Pf6N2E2_1110 +0.6 3.2 FIG00964414: hypothetical protein compare
Pf6N2E2_4838 +0.6 4.2 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
Pf6N2E2_4305 +0.6 1.0 FIG00959590: hypothetical protein compare
Pf6N2E2_2207 +0.6 2.5 cytochrome b(561) compare
Pf6N2E2_4054 +0.6 1.4 Osmotically inducible protein C compare
Pf6N2E2_4427 +0.6 2.1 FIG00954215: hypothetical protein compare
Pf6N2E2_2640 +0.6 1.5 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3338 +0.6 1.0 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_4810 +0.6 2.8 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_4710 +0.6 1.7 hypothetical protein compare
Pf6N2E2_405 +0.6 0.4 hypothetical protein compare
Pf6N2E2_5771 +0.6 3.4 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_4120 +0.6 4.6 Trk system potassium uptake protein TrkA compare
Pf6N2E2_5646 +0.6 1.2 PROBABLE MEMBRANE PROTEIN compare
Pf6N2E2_2645 +0.6 2.5 ATP-dependent DNA helicase RecQ compare


Specific Phenotypes

For 7 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas fluorescens FW300-N2E2

For stress Nalidixic acid sodium salt across organisms