Experiment set3IT070 for Pseudomonas fluorescens FW300-N2E2

LB with Nalidixic acid sodium salt 0.004 mg/ml

Group: stress
Media: LB + Nalidixic acid sodium salt (0.004 mg/ml)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.9 generations
By: Mark on 3/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: plate1 C4

Specific Phenotypes

For 7 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas fluorescens FW300-N2E2

For stress Nalidixic acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Carboxysome	1
DNA Repair Base Excision	1
DNA repair, bacterial	1
DNA repair, bacterial DinG and relatives	1
Peptidoglycan Biosynthesis	1
Respiratory dehydrogenases 1	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Oxidative phosphorylation
• Lysine degradation
• Biotin metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NADH to cytochrome bo oxidase electron transfer I	2	2	1
NADH to cytochrome bd oxidase electron transfer I	2	1	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
aerobic respiration I (cytochrome c)	4	3	1
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	3	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1