Experiment set3IT070 for Pseudomonas fluorescens FW300-N2E3

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LB with Fusidic acid sodium salt 0.125 mg/ml

Group: stress
Media: LB + Fusidic acid sodium salt (0.125 mg/ml)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 6/18/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 944 E5,E6

Specific Phenotypes

For 27 genes in this experiment

For stress Fusidic acid sodium salt in Pseudomonas fluorescens FW300-N2E3

For stress Fusidic acid sodium salt across organisms

SEED Subsystems

Subsystem #Specific
Oxidative stress 2
ATP-dependent RNA helicases, bacterial 1
Bacterial Cell Division 1
Copper homeostasis 1
DNA Repair Base Excision 1
Glycine and Serine Utilization 1
Heat shock dnaK gene cluster extended 1
Lipid A modifications 1
Peptidoglycan Biosynthesis 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Ribosome biogenesis bacterial 1
Serine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-glucose biosynthesis 2 2 1
L-serine biosynthesis I 3 3 1
GDP-α-D-glucose biosynthesis 3 2 1
L-cysteine biosynthesis IX (Trichomonas vaginalis) 3 2 1
trehalose degradation V 3 2 1
superpathway of L-serine and glycine biosynthesis I 4 4 1
queuosine biosynthesis I (de novo) 4 4 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
glycogen biosynthesis I (from ADP-D-Glucose) 4 3 1
starch degradation V 4 3 1
starch degradation III 4 2 1
dTDP-β-L-rhamnose biosynthesis 5 5 1
sucrose degradation II (sucrose synthase) 5 4 1
glucose and glucose-1-phosphate degradation 5 4 1
queuosine biosynthesis III (queuosine salvage) 5 3 1
D-galactose degradation I (Leloir pathway) 5 2 1
glucosylglycerol biosynthesis 5 2 1
CDP-6-deoxy-D-gulose biosynthesis 5 1 1
glycogen degradation II 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
sucrose biosynthesis II 8 6 1
glycogen degradation I 8 6 1
glycogen biosynthesis III (from α-maltose 1-phosphate) 8 3 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
chitin biosynthesis 9 5 1
starch biosynthesis 10 5 1
O-antigen building blocks biosynthesis (E. coli) 11 10 1
colanic acid building blocks biosynthesis 11 9 1
streptomycin biosynthesis 18 2 1
superpathway of anaerobic sucrose degradation 19 15 1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis 19 5 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 13 1