Experiment set3IT070 for Dyella japonica UNC79MFTsu3.2

LB with D-Cycloserine 0.032 mg/ml

Group: stress
Media: LB + D-Cycloserine (0.032 mg/ml)
Culturing: Dyella79_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.2 generations
By: Adam on 17-May-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1773 C2

Specific Phenotypes

For 4 genes in this experiment

For stress D-Cycloserine in Dyella japonica UNC79MFTsu3.2

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem	#Specific
Control of cell elongation - division cycle in Bacilli	1
DNA Repair Base Excision	1
TCA Cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
TCA cycle VIII (Chlamydia)	6	5	1
incomplete reductive TCA cycle	7	3	1
pyruvate fermentation to propanoate I	7	3	1
partial TCA cycle (obligate autotrophs)	8	7	1
TCA cycle V (2-oxoglutarate synthase)	9	8	1
TCA cycle II (plants and fungi)	9	8	1
TCA cycle IV (2-oxoglutarate decarboxylase)	9	8	1
TCA cycle VI (Helicobacter)	9	6	1
TCA cycle VII (acetate-producers)	9	6	1
TCA cycle III (animals)	10	9	1
TCA cycle I (prokaryotic)	10	8	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	5	1
reductive TCA cycle I	11	6	1
L-glutamate degradation VIII (to propanoate)	11	3	1
superpathway of glyoxylate bypass and TCA	12	10	1
reductive TCA cycle II	12	6	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	4	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
mixed acid fermentation	16	11	1
superpathway of anaerobic energy metabolism (invertebrates)	17	12	1
methylaspartate cycle	19	11	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	19	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	23	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	1