Experiment set3IT067 for Acidovorax sp. GW101-3H11

LB with 2-Furfuraldehyde 0.06 vol%

Group: stress
Media: LB + 2-Furfuraldehyde (0.06 vol%)
Culturing: acidovorax_3H11_ML3a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.1 generations
By: Mark on 5/19/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 6 genes in this experiment

For stress 2-Furfuraldehyde in Acidovorax sp. GW101-3H11

For stress 2-Furfuraldehyde across organisms

SEED Subsystems

Subsystem	#Specific
Arginine Biosynthesis extended	1
Cobalt-zinc-cadmium resistance	1
Methionine Biosynthesis	1
Nudix proteins (nucleoside triphosphate hydrolases)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Methionine metabolism
• Geraniol degradation
• Lysine biosynthesis
• Tryptophan metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• One carbon pool by folate
• Limonene and pinene degradation
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-methionine salvage from L-homocysteine	3	1	1
L-methionine biosynthesis III	4	4	1
L-ornithine biosynthesis I	5	5	1
L-methionine biosynthesis I	5	3	1
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
folate transformations III (E. coli)	9	7	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
folate transformations II (plants)	11	8	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
folate transformations I	13	7	1
superpathway of L-methionine salvage and degradation	16	7	1
superpathway of arginine and polyamine biosynthesis	17	15	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	22	1