Experiment set3IT066 for Burkholderia phytofirmans PsJN
L-Phenylalanine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Phenylalanine (20 mM), pH=7
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.0 generations
By: Mark on 1/27/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Plate 1 C6
Specific Phenotypes
For 7 genes in this experiment
For nitrogen source L-Phenylalanine in Burkholderia phytofirmans PsJN
For nitrogen source L-Phenylalanine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ABC transporter branched-chain amino acid (TC 3.A.1.4.1) | 3 |
| Aromatic Amin Catabolism | 1 |
| Aromatic amino acid interconversions with aryl acids | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
- Lysine degradation
- Tryptophan metabolism
- beta-Alanine metabolism
- Propanoate metabolism
- Butanoate metabolism
- Limonene and pinene degradation
- Glycolysis / Gluconeogenesis
- Ascorbate and aldarate metabolism
- Fatty acid elongation in mitochondria
- Urea cycle and metabolism of amino groups
- Geraniol degradation
- Arginine and proline metabolism
- Histidine metabolism
- Glycerolipid metabolism
- alpha-Linolenic acid metabolism
- Pyruvate metabolism
- 1,2-Dichloroethane degradation
- Benzoate degradation via CoA ligation
- 3-Chloroacrylic acid degradation
- Caprolactam degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: