Experiment set3IT065 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Tetracycline hydrochloride 0.0001 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5499 +1.2 2.8 hypothetical protein compare
Pf6N2E2_5766 +1.0 2.0 Quaternary ammonium compound-resistance protein SugE compare
Pf6N2E2_2509 +0.9 2.6 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_3325 +0.8 2.2 ABC transporter, ATP-binding protein compare
Pf6N2E2_3033 +0.8 1.6 hypothetical protein compare
Pf6N2E2_1981 +0.8 3.7 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_819 +0.8 2.4 Response regulator compare
Pf6N2E2_4640 +0.8 1.6 hypothetical protein compare
Pf6N2E2_4057 +0.8 1.1 lipoprotein, putative compare
Pf6N2E2_3659 +0.8 4.4 Protein fixF compare
Pf6N2E2_5555 +0.8 1.7 Cold shock protein CspA compare
Pf6N2E2_2513 +0.7 3.0 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_1248 +0.7 2.7 MutT/nudix family protein compare
Pf6N2E2_923 +0.7 2.4 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_2022 +0.7 4.9 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_4743 +0.7 1.2 hypothetical protein compare
Pf6N2E2_1056 +0.7 1.8 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_2507 +0.7 4.4 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_1983 +0.7 2.7 hypothetical protein compare
Pf6N2E2_605 +0.7 2.8 FIG002465: BNR repeat protein compare
Pf6N2E2_420 +0.7 1.8 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_5714 +0.7 2.3 Flavoprotein WrbA compare
Pf6N2E2_3475 +0.6 1.2 hypothetical protein compare
Pf6N2E2_2106 +0.6 1.8 hypothetical protein compare
Pf6N2E2_3663 +0.6 4.2 Glycosyl transferase compare
Pf6N2E2_3791 +0.6 4.9 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_2506 +0.6 5.3 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_613 +0.6 1.5 lipoprotein, putative compare
Pf6N2E2_5044 +0.6 2.8 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_2578 +0.6 1.9 Flagellar biosynthesis protein FliR compare
Pf6N2E2_2438 +0.6 2.0 hypothetical protein compare
Pf6N2E2_5226 +0.6 0.9 FIG00955589: hypothetical protein compare
Pf6N2E2_4427 +0.6 2.2 FIG00954215: hypothetical protein compare
Pf6N2E2_3338 +0.6 1.8 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_5455 +0.6 2.0 hypothetical protein compare
Pf6N2E2_2375 +0.5 1.8 Response regulator NasT compare
Pf6N2E2_2589 +0.5 2.7 Flagellar protein FliJ compare
Pf6N2E2_5720 +0.5 4.6 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_3537 +0.5 3.2 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf6N2E2_5160 +0.5 2.0 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5613 +0.5 1.6 FIG00953674: hypothetical protein compare
Pf6N2E2_775 +0.5 1.8 FIG00955061: hypothetical protein compare
Pf6N2E2_5077 +0.5 2.5 hypothetical protein compare
Pf6N2E2_445 +0.5 2.8 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_6090 +0.5 0.8 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_1074 +0.5 1.8 FIG00953356: hypothetical protein compare
Pf6N2E2_888 +0.5 1.9 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_3944 +0.5 2.3 Protein of unknown function DUF81 compare
Pf6N2E2_2013 +0.5 1.1 hypothetical protein compare
Pf6N2E2_5246 +0.5 1.4 FIG00955938: hypothetical protein compare
Pf6N2E2_5279 +0.5 2.5 Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C) compare
Pf6N2E2_3875 +0.5 1.0 FIG00953324: hypothetical protein compare
Pf6N2E2_4332 +0.5 2.6 hypothetical protein compare
Pf6N2E2_3457 +0.5 1.4 Uncharacterized protein YfiR precursor compare
Pf6N2E2_2510 +0.5 2.0 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_5342 +0.5 1.7 21 kDa hemolysin precursor compare
Pf6N2E2_6050 +0.5 2.8 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_4077 +0.5 1.3 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_1968 +0.5 1.5 Cobalt-zinc-cadmium resistance protein compare
Pf6N2E2_4541 +0.5 1.1 hypothetical protein compare
Pf6N2E2_6081 +0.5 1.6 Transcriptional regulator, TetR family, in cluster with 2-hydroxychromene-2-carboxylate isomerase family protein, glutathione-dependent compare
Pf6N2E2_5256 +0.5 3.3 FIG137478: Hypothetical protein YbgI compare
Pf6N2E2_1602 +0.5 2.1 hypothetical protein compare
Pf6N2E2_98 +0.5 1.4 putative transmembrane protein compare
Pf6N2E2_1715 +0.5 1.2 hypothetical protein compare
Pf6N2E2_5525 +0.5 3.7 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases compare
Pf6N2E2_4050 +0.5 1.9 FIG00953718: hypothetical protein compare
Pf6N2E2_6085 +0.4 1.2 Aha1 domain superfamily compare
Pf6N2E2_414 +0.4 2.2 Chemotaxis protein CheD compare
Pf6N2E2_1706 +0.4 1.4 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_5545 +0.4 2.5 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_5243 +0.4 1.4 hypothetical protein compare
Pf6N2E2_3792 +0.4 3.0 Glucans biosynthesis protein G precursor compare
Pf6N2E2_2592 +0.4 2.8 Flagellar motor switch protein FliG compare
Pf6N2E2_786 +0.4 1.9 Type-1 fimbrial protein, A chain precursor compare
Pf6N2E2_4531 +0.4 1.2 hypothetical protein compare
Pf6N2E2_552 +0.4 1.2 Uncharacterized protein ImpF compare
Pf6N2E2_2811 +0.4 3.2 FIG00956006: hypothetical protein compare
Pf6N2E2_726 +0.4 1.8 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_2519 +0.4 1.0 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_773 +0.4 3.0 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_1497 +0.4 2.5 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_282 +0.4 0.9 GNAT family acetyltransferase YjcF compare
Pf6N2E2_3105 +0.4 2.5 hypothetical protein compare
Pf6N2E2_5095 +0.4 2.5 Putative inner membrane protein (Fragment) compare
Pf6N2E2_1502 +0.4 1.1 Two-component system regulatory protein compare
Pf6N2E2_3744 +0.4 1.7 General secretion pathway protein F compare
Pf6N2E2_187 +0.4 2.2 Urea ABC transporter, ATPase protein UrtE compare
Pf6N2E2_623 +0.4 0.9 FIG00954674: hypothetical protein compare
Pf6N2E2_2599 +0.4 2.6 Flagellar biosynthesis protein FliS compare
Pf6N2E2_1258 +0.4 1.4 FIG00960119: hypothetical protein compare
Pf6N2E2_2420 +0.4 1.3 hypothetical protein compare
Pf6N2E2_1674 +0.4 2.3 Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) compare
Pf6N2E2_5024 +0.4 1.3 Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7) compare
Pf6N2E2_882 +0.4 1.8 FIG00961517: hypothetical protein compare
Pf6N2E2_5301 +0.4 2.4 FIG00957554: hypothetical protein compare
Pf6N2E2_1804 +0.4 2.3 Immune-responsive protein 1 compare
Pf6N2E2_5730 +0.4 1.5 FIG00954480: hypothetical protein compare
Pf6N2E2_757 +0.4 2.3 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) compare
Pf6N2E2_228 +0.4 2.2 Transcriptional regulator, IclR family compare
Pf6N2E2_3480 +0.4 1.0 Transcriptional regulator, ArsR family compare
Pf6N2E2_5505 +0.4 1.3 hypothetical protein compare
Pf6N2E2_5207 +0.4 0.9 hypothetical protein compare
Pf6N2E2_1749 +0.4 1.8 Cyn operon transcriptional activator compare
Pf6N2E2_5991 +0.4 1.6 proteinase inhibitor compare
Pf6N2E2_945 +0.4 1.4 Transcriptional regulator, TetR family compare
Pf6N2E2_2002 +0.4 2.2 hypothetical protein compare
Pf6N2E2_1778 +0.4 1.7 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_251 +0.4 1.8 transcriptional regulator compare
Pf6N2E2_6093 +0.4 1.5 hypothetical protein compare
Pf6N2E2_3870 +0.4 0.9 hypothetical protein compare
Pf6N2E2_5755 +0.4 1.4 FIG00956435: hypothetical protein compare
Pf6N2E2_6077 +0.4 2.0 Cysteine desulfurase (EC 2.8.1.7) compare
Pf6N2E2_959 +0.4 1.5 putative membrane protein compare
Pf6N2E2_2693 +0.4 0.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_4700 +0.4 2.1 Microsomal dipeptidase (EC 3.4.13.19) compare
Pf6N2E2_1361 +0.4 1.6 Periplasmic protein p19 involved in high-affinity Fe2+ transport compare
Pf6N2E2_1335 +0.4 1.3 Phospholipid-binding protein compare
Pf6N2E2_4548 +0.4 1.6 hypothetical protein compare
Pf6N2E2_3271 +0.4 1.3 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_342 +0.4 0.7 DNA-binding response regulator compare
Pf6N2E2_1432 +0.4 1.3 High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1) compare
Pf6N2E2_4814 +0.4 1.9 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_255 +0.4 2.3 DoxX family protein compare
Pf6N2E2_2583 +0.4 2.1 Flagellar motor switch protein FliM compare
Pf6N2E2_1162 +0.4 0.8 Transmembrane amino acid efflux protein compare
Pf6N2E2_4364 +0.4 1.5 Protein YicC compare
Pf6N2E2_3957 +0.4 1.8 Putative membrane-bound ClpP-class protease associated with aq_911 compare
Pf6N2E2_4895 +0.4 2.0 Isochorismatase (EC 3.3.2.1) compare
Pf6N2E2_5346 +0.4 1.1 rRNA small subunit methyltransferase I compare
Pf6N2E2_2508 +0.4 1.6 hypothetical protein compare
Pf6N2E2_444 +0.4 1.6 hypothetical protein compare
Pf6N2E2_952 +0.4 1.8 putative exported protein compare
Pf6N2E2_3277 +0.3 1.3 FagA protein compare
Pf6N2E2_5848 +0.3 1.7 small component of pyruvate transporter (yjcH-like) (from data) compare
Pf6N2E2_2572 +0.3 1.5 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_144 +0.3 3.3 Uncharacterized protein YtfM precursor compare
Pf6N2E2_5695 +0.3 2.3 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_2597 +0.3 2.8 Flagellar regulatory protein FleQ compare
Pf6N2E2_5476 +0.3 1.0 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_1665 +0.3 1.2 hypothetical protein compare
Pf6N2E2_2502 +0.3 1.2 O-antigen acetylase compare
Pf6N2E2_2600 +0.3 2.8 Flagellar hook-associated protein FliD compare
Pf6N2E2_2373 +0.3 0.7 Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid) compare
Pf6N2E2_1612 +0.3 2.3 Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1) compare
Pf6N2E2_205 +0.3 1.9 hypothetical protein compare
Pf6N2E2_6023 +0.3 1.9 Putative cytoplasmic protein compare
Pf6N2E2_2580 +0.3 1.7 Flagellar biosynthesis protein FliP compare
Pf6N2E2_2577 +0.3 2.7 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_3770 +0.3 1.2 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_5761 +0.3 1.1 Transcriptional regulator, TetR family compare
Pf6N2E2_3019 +0.3 1.8 FIG00954559: hypothetical protein compare
Pf6N2E2_1404 +0.3 1.4 Nitrous oxide reductase maturation transmembrane protein NosY compare
Pf6N2E2_571 +0.3 1.6 ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) compare
Pf6N2E2_1452 +0.3 1.6 hypothetical protein compare
Pf6N2E2_5999 +0.3 1.4 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9) compare
Pf6N2E2_4556 +0.3 0.9 FIG00953078: hypothetical protein compare
Pf6N2E2_5843 +0.3 0.8 hypothetical protein compare
Pf6N2E2_6080 +0.3 1.7 Short-chain dehydrogenase, associated with 2-hydroxychromene-2-carboxylate isomerase family protein compare
Pf6N2E2_1371 +0.3 1.1 hypothetical protein compare
Pf6N2E2_3713 +0.3 0.8 Acyl carrier protein compare
Pf6N2E2_1762 +0.3 1.3 putative lipase compare
Pf6N2E2_5689 +0.3 1.7 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_1696 +0.3 1.2 Alkanesulfonates-binding protein compare
Pf6N2E2_2518 +0.3 0.8 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_5576 +0.3 1.6 Cys-tRNA(Pro) deacylase YbaK compare
Pf6N2E2_142 +0.3 1.8 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_264 +0.3 1.0 Transcriptional regulator, TetR family compare
Pf6N2E2_2281 +0.3 1.1 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_3226 +0.3 1.4 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_6029 +0.3 0.9 hypothetical protein compare
Pf6N2E2_5694 +0.3 2.2 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_1765 +0.3 1.5 hypothetical protein compare
Pf6N2E2_4810 +0.3 2.0 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_2861 +0.3 1.5 hypothetical protein compare
Pf6N2E2_3702 +0.3 1.4 FIG00953907: hypothetical protein compare
Pf6N2E2_2822 +0.3 2.1 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_1591 +0.3 1.9 hypothetical protein compare
Pf6N2E2_4448 +0.3 1.5 probable P23 protein compare
Pf6N2E2_2857 +0.3 1.7 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) compare
Pf6N2E2_3852 +0.3 1.9 Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-) compare
Pf6N2E2_37 +0.3 1.1 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_2155 +0.3 1.8 hypothetical protein compare
Pf6N2E2_1424 +0.3 1.0 transcriptional regulator, TetR family compare
Pf6N2E2_2640 +0.3 0.9 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_2536 +0.3 0.6 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_5920 +0.3 1.4 Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly compare
Pf6N2E2_2603 +0.3 2.1 hypothetical protein compare
Pf6N2E2_3981 +0.3 2.9 T1SS secreted agglutinin RTX compare
Pf6N2E2_5164 +0.3 2.1 Periplasmic hemin-binding protein compare
Pf6N2E2_2624 +0.3 2.1 Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-) compare
Pf6N2E2_4534 +0.3 1.7 hypothetical protein compare
Pf6N2E2_5094 +0.3 1.5 FIG00956889: hypothetical protein compare
Pf6N2E2_216 +0.3 1.5 Copper-sensing two-component system response regulator CpxR compare
Pf6N2E2_3978 +0.3 2.4 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_2591 +0.3 1.4 Flagellar assembly protein FliH compare
Pf6N2E2_1386 +0.3 1.8 amino acid ABC transporter, periplasmic substrate-binding protein compare
Pf6N2E2_4546 +0.3 1.8 hypothetical protein compare
Pf6N2E2_703 +0.3 1.5 Cytochrome c55X precursor NirC compare
Pf6N2E2_1246 +0.3 1.1 FIG00961372: hypothetical protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E2 in stress experiments

For stress Tetracycline hydrochloride across organisms