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Experiment set3IT063 for
Methanococcus maripaludis S2
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS08860
+1.2
8.5
MinD/ParA family protein
compare
MMP
_RS08940
+1.2
3.0
MetS family NSS transporter small subunit
compare
MMP
_RS09055
+1.1
2.4
CAP domain-containing protein
compare
MMP
_RS08855
+1.1
8.6
hypothetical protein
compare
MMP
_RS01290
+1.1
9.6
class III signal peptide-containing protein
compare
MMP
_RS00240
+1.0
8.9
type II secretion system F family protein
compare
MMP
_RS00245
+1.0
9.3
type II secretion system F family protein
compare
MMP
_RS01285
+1.0
8.6
hypothetical protein
compare
MMP
_RS01280
+1.0
8.3
DUF515 domain-containing protein
compare
MMP
_RS01305
+1.0
9.1
hypothetical protein
compare
MMP
_RS08615
+1.0
2.2
flagellar protein G
compare
MMP
_RS01270
+0.9
8.1
prepilin peptidase
compare
MMP
_RS01315
+0.9
8.8
DUF2341 domain-containing protein
compare
MMP
_RS01310
+0.9
8.7
hypothetical protein
compare
MMP
_RS00250
+0.9
8.0
Flp pilus assembly complex ATPase component TadA
compare
MMP
_RS01195
+0.9
2.2
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS01085
+0.9
3.1
hypothetical protein
compare
MMP
_RS01275
+0.9
7.3
class III signal peptide-containing protein
compare
MMP
_RS08675
+0.9
3.8
class III signal peptide-containing protein
compare
MMP
_RS01295
+0.9
7.7
hypothetical protein
compare
MMP
_RS07330
+0.8
7.5
membrane protein
compare
MMP
_RS02605
+0.8
1.1
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS05985
+0.8
3.2
hypothetical protein
compare
MMP
_RS03125
+0.7
4.3
cell division ATPase MinD
compare
MMP
_RS08580
+0.7
5.2
flagellin
compare
MMP
_RS05635
+0.7
5.0
NAD-dependent epimerase/dehydratase family protein
compare
MMP
_RS01075
+0.6
1.7
hypothetical protein
compare
MMP
_RS01790
+0.6
3.3
restriction endonuclease
compare
MMP
_RS08750
+0.6
1.4
hypothetical protein
compare
MMP
_RS08400
+0.6
1.5
MJ0307 family thioredoxin
compare
MMP
_RS05355
+0.6
0.9
hypothetical protein
compare
MMP
_RS08845
+0.6
3.9
archaellum operon transcriptional activator EarA family protein
compare
MMP
_RS01570
+0.5
1.4
hypothetical protein
compare
MMP
_RS01630
+0.5
4.4
N-glycosylase/DNA lyase
compare
MMP
_RS09045
+0.5
4.3
class III signal peptide-containing protein
compare
MMP
_RS00570
+0.5
0.9
protein-L-isoaspartate O-methyltransferase
compare
MMP
_RS07345
+0.5
2.0
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS03550
+0.5
1.3
DUF2124 domain-containing protein
compare
MMP
_RS07900
+0.5
1.6
YeeE/YedE family protein
compare
MMP
_RS00535
+0.5
2.6
TIGR00375 family protein
compare
MMP
_RS01650
+0.5
0.6
sulfurtransferase TusA family protein
compare
MMP
_RS08410
+0.5
2.8
DUF169 domain-containing protein
compare
MMP
_RS00655
+0.5
2.3
hypothetical protein
compare
MMP
_RS04410
+0.5
2.1
hypothetical protein
compare
MMP
_RS08830
+0.5
2.4
GTP-binding protein
compare
MMP
_RS07765
+0.4
2.6
sodium:alanine symporter family protein
compare
MMP
_RS06035
+0.4
2.7
glycosyltransferase family 2 protein
compare
MMP
_RS05865
+0.4
1.3
hypothetical protein
compare
MMP
_RS05140
+0.4
1.8
helix-turn-helix transcriptional regulator
compare
MMP
_RS00140
+0.4
2.3
DUF192 domain-containing protein
compare
MMP
_RS08080
+0.4
2.6
YchF/TatD family DNA exonuclease
compare
MMP
_RS06965
+0.4
3.3
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS07840
+0.4
1.9
ribonuclease III
compare
MMP
_RS08285
+0.4
2.6
serine/threonine protein kinase
compare
MMP
_RS06665
+0.4
2.8
glycogen/starch synthase
compare
MMP
_RS04125
+0.4
2.1
TetR/AcrR family transcriptional regulator
compare
MMP
_RS06175
+0.4
2.4
winged helix-turn-helix transcriptional regulator
compare
MMP
_RS08850
+0.4
3.7
hypothetical protein
compare
MMP
_RS08160
+0.4
2.2
EamA family transporter
compare
MMP
_RS06935
+0.4
2.2
protease complex subunit PrcB family protein
compare
MMP
_RS08395
+0.4
1.6
DsrE family protein
compare
MMP
_RS07775
+0.4
1.4
alanine dehydrogenase
compare
MMP
_RS02545
+0.4
2.0
hypothetical protein
compare
MMP
_RS08770
+0.4
2.6
hypothetical protein
compare
MMP
_RS07550
+0.4
2.4
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS06760
+0.4
1.0
hypothetical protein
compare
MMP
_RS07880
+0.4
2.4
AAA family ATPase
compare
MMP
_RS08700
+0.4
2.5
hypothetical protein
compare
MMP
_RS06990
+0.4
2.0
CatB-related O-acetyltransferase
compare
MMP
_RS00690
+0.4
1.9
MBL fold metallo-hydrolase
compare
MMP
_RS07925
+0.4
1.9
haloacid dehalogenase
compare
MMP
_RS01255
+0.3
1.8
amino acid ABC transporter ATP-binding protein
compare
MMP
_RS04030
+0.3
1.9
hypothetical protein
compare
MMP
_RS01110
+0.3
1.0
FmdE family protein
compare
MMP
_RS08385
+0.3
1.7
ABC transporter substrate-binding protein
compare
MMP
_RS06325
+0.3
0.8
hypothetical protein
compare
MMP
_RS01675
+0.3
1.0
hypothetical protein
compare
MMP
_RS01870
+0.3
1.7
acyl-CoA dehydratase activase
compare
MMP
_RS01125
+0.3
1.8
thioredoxin family protein
compare
MMP
_RS08665
+0.3
2.8
hypothetical protein
compare
MMP
_RS01655
+0.3
1.2
DsrE family protein
compare
MMP
_RS01635
+0.3
2.2
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS08485
+0.3
2.1
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS03535
+0.3
1.0
helix-turn-helix transcriptional regulator
compare
MMP
_RS05785
+0.3
2.6
ATP-binding protein
compare
MMP
_RS03080
+0.3
0.6
universal stress protein
compare
MMP
_RS01490
+0.3
1.9
tetratricopeptide repeat protein
compare
MMP
_RS02425
+0.3
2.0
hypothetical protein
compare
MMP
_RS02735
+0.3
1.9
MBL fold metallo-hydrolase
compare
MMP
_RS02530
+0.3
1.7
type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family
compare
MMP
_RS08130
+0.3
2.3
IGHMBP2 family helicase
compare
MMP
_RS07985
+0.3
2.6
nitroreductase family protein
compare
MMP
_RS08775
+0.3
1.8
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS03625
+0.3
1.7
cell wall-binding protein
compare
MMP
_RS06050
+0.3
1.9
ZIP family metal transporter
compare
MMP
_RS06585
+0.3
0.8
DMT family protein
compare
MMP
_RS08265
+0.3
1.6
rubrerythrin
compare
MMP
_RS06710
+0.3
1.8
selenium-binding protein
compare
MMP
_RS01320
+0.3
1.7
hypothetical protein
compare
MMP
_RS07590
+0.3
2.3
hypothetical protein
compare
MMP
_RS07415
+0.3
1.3
molybdopterin-guanine dinucleotide biosynthesis protein MobB
compare
MMP
_RS03965
+0.3
1.0
hypothetical protein
compare
MMP
_RS08405
+0.3
1.9
MFS transporter
compare
MMP
_RS08260
+0.3
2.0
DUF99 family protein
compare
MMP
_RS02255
+0.3
1.8
hypothetical protein
compare
MMP
_RS08610
+0.3
2.2
flagellar protein F
compare
MMP
_RS02295
+0.3
2.2
TatD family hydrolase
compare
MMP
_RS07800
+0.3
1.3
ATP-binding cassette domain-containing protein
compare
MMP
_RS01135
+0.3
2.0
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS01605
+0.3
1.7
hypothetical protein
compare
MMP
_RS08235
+0.3
1.9
hypothetical protein
compare
MMP
_RS08075
+0.3
1.5
DUF749 domain-containing protein
compare
MMP
_RS06910
+0.3
1.9
RlmF-related methyltransferase
compare
MMP
_RS08280
+0.3
1.2
proteasome assembly chaperone family protein
compare
MMP
_RS02020
+0.3
1.6
gamma-glutamylcyclotransferase
compare
MMP
_RS02260
+0.3
1.8
lactate utilization protein
compare
MMP
_RS08380
+0.3
1.5
phosphatase PAP2 family protein
compare
MMP
_RS06460
+0.3
0.6
pyrimidine dimer DNA glycosylase/endonuclease V
compare
MMP
_RS05780
+0.3
1.8
multidrug resistance efflux transporter family protein
compare
MMP
_RS06015
+0.3
1.0
flavodoxin family protein
compare
MMP
_RS06115
+0.3
1.9
class II aldolase/adducin family protein
compare
MMP
_RS00395
+0.2
0.7
MGMT family protein
compare
MMP
_RS02680
+0.2
1.5
ABC transporter permease
compare
MMP
_RS05715
+0.2
1.6
hypothetical protein
compare
MMP
_RS06140
+0.2
0.9
radical SAM protein
compare
MMP
_RS07915
+0.2
1.3
DUF4870 domain-containing protein
compare
MMP
_RS08310
+0.2
0.8
TMEM165/GDT1 family protein
compare
MMP
_RS05965
+0.2
1.1
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS01300
+0.2
2.1
hypothetical protein
compare
MMP
_RS02215
+0.2
1.7
ECF transporter S component
compare
MMP
_RS08490
+0.2
2.0
hypothetical protein
compare
MMP
_RS02770
+0.2
1.2
hypothetical protein
compare
MMP
_RS09065
+0.2
2.0
hypothetical protein
compare
MMP
_RS04255
+0.2
1.1
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS02455
+0.2
1.4
hypothetical protein
compare
MMP
_RS07150
+0.2
1.3
MTH895/ArsE family thioredoxin-like protein
compare
MMP
_RS00390
+0.2
1.5
ammonium transporter
compare
MMP
_RS06720
+0.2
1.3
response regulator transcription factor
compare
MMP
_RS07895
+0.2
1.1
cytochrome c
compare
MMP
_RS06100
+0.2
1.6
GNAT family N-acetyltransferase
compare
MMP
_RS06005
+0.2
1.3
heavy-metal-associated domain-containing protein
compare
MMP
_RS02670
+0.2
1.6
ATP-binding cassette domain-containing protein
compare
MMP
_RS04190
+0.2
1.2
hypothetical protein
compare
MMP
_RS08250
+0.2
1.4
pyruvate kinase
compare
MMP
_RS08390
+0.2
1.1
DUF5400 domain-containing protein
compare
MMP
_RS02740
+0.2
1.0
hypothetical protein
compare
MMP
_RS08205
+0.2
1.7
stage II sporulation protein M
compare
MMP
_RS06735
+0.2
1.7
class I SAM-dependent rRNA methyltransferase
compare
MMP
_RS04530
+0.2
1.5
ABC transporter permease
compare
MMP
_RS06465
+0.2
1.8
NERD domain-containing protein
compare
MMP
_RS00545
+0.2
1.1
XRE family transcriptional regulator
compare
MMP
_RS01685
+0.2
0.9
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS07805
+0.2
1.2
ABC transporter permease
compare
MMP
_RS07715
+0.2
1.7
YfcE family phosphodiesterase
compare
MMP
_RS00675
+0.2
0.3
nitroreductase family protein
compare
MMP
_RS01770
+0.2
1.7
ATP-dependent endonuclease
compare
MMP
_RS06190
+0.2
1.4
tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
compare
MMP
_RS06095
+0.2
1.3
ABC transporter ATP-binding protein
compare
MMP
_RS06090
+0.2
1.1
iron ABC transporter permease
compare
MMP
_RS03060
+0.2
1.2
MBL fold metallo-hydrolase
compare
MMP
_RS04205
+0.2
1.1
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS05910
+0.2
0.9
cell division ATPase MinD
compare
MMP
_RS08480
+0.2
1.5
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS07155
+0.2
1.3
MFS transporter
compare
MMP
_RS06930
+0.2
1.6
MBL fold metallo-hydrolase
compare
MMP
_RS05970
+0.2
0.9
class II SORL domain-containing protein
compare
MMP
_RS01420
+0.2
0.7
hypothetical protein
compare
MMP
_RS08640
+0.2
1.5
TIM barrel protein
compare
MMP
_RS07905
+0.2
1.2
DUF3343 domain-containing protein
compare
MMP
_RS02320
+0.2
1.3
glutamate-cysteine ligase family protein
compare
MMP
_RS07595
+0.2
1.6
hypothetical protein
compare
MMP
_RS01090
+0.2
1.3
ABC transporter substrate-binding protein
compare
MMP
_RS08555
+0.2
1.0
hypothetical protein
compare
MMP
_RS00295
+0.2
0.6
gamma carbonic anhydrase family protein
compare
MMP
_RS08575
+0.2
1.7
hypothetical protein
compare
MMP
_RS00090
+0.2
0.7
CoA-binding protein
compare
MMP
_RS06320
+0.2
1.0
hypothetical protein
compare
MMP
_RS08980
+0.2
0.8
hypothetical protein
compare
MMP
_RS05580
+0.2
1.7
glycosyltransferase
compare
MMP
_RS07910
+0.2
1.1
NAD(P)H-hydrate dehydratase
compare
MMP
_RS08670
+0.2
1.1
metallophosphoesterase
compare
MMP
_RS05975
+0.2
0.5
hypothetical protein
compare
MMP
_RS06440
+0.2
1.1
CBS domain-containing protein
compare
MMP
_RS06540
+0.2
0.8
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS06520
+0.2
1.0
DUF1786 domain-containing protein
compare
MMP
_RS04200
+0.2
1.2
DUF111 family protein
compare
MMP
_RS04145
+0.2
0.5
hypothetical protein
compare
MMP
_RS00660
+0.2
1.3
alkaline phosphatase family protein
compare
MMP
_RS00540
+0.2
1.0
PLP-dependent aminotransferase family protein
compare
MMP
_RS04995
+0.2
1.1
hypothetical protein
compare
MMP
_RS05770
+0.2
1.1
YkgJ family cysteine cluster protein
compare
MMP
_RS06185
+0.2
1.2
thermonuclease family protein
compare
MMP
_RS02385
+0.2
1.2
ATP-binding protein
compare
MMP
_RS07770
+0.2
1.0
alanine racemase
compare
MMP
_RS01660
+0.2
0.7
hypothetical protein
compare
MMP
_RS01855
+0.2
1.2
2-hydroxyacyl-CoA dehydratase family protein
compare
MMP
_RS05235
+0.2
0.9
exodeoxyribonuclease III
compare
MMP
_RS01765
+0.2
0.9
HIT family protein
compare
MMP
_RS02695
+0.2
1.1
formylmethanofuran dehydrogenase subunit A
compare
MMP
_RS01105
+0.2
1.0
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t1 experiments