Experiment set3IT062 for Rhodanobacter denitrificans FW104-10B01

R2A with Cobalt chloride hexahydrate 0.15625 mM

Group: stress
Media: R2A + Cobalt chloride hexahydrate (0.15625 mM)
Culturing: rhodanobacter_10B01_ML12, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
By: Hans on 2/27/21
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 12 genes in this experiment

For stress Cobalt chloride hexahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Campylobacter Iron Metabolism	1
Cobalt-zinc-cadmium resistance	1
Heat shock dnaK gene cluster extended	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Pyrimidine utilization	1
Trehalose Uptake and Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Puromycin biosynthesis
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
β-alanine degradation III	2	1	1
uracil degradation III	5	3	1
3-hydroxypropanoate cycle	13	7	1
glyoxylate assimilation	13	5	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	10	1
superpathway of the 3-hydroxypropanoate cycle	18	7	1