Experiment set3IT061 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Rifampicin 0.003 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_3659 +11.3 35.1 Protein fixF compare
Pf6N2E2_3663 +11.3 64.6 Glycosyl transferase compare
Pf6N2E2_3658 +9.7 29.8 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_3656 +8.9 47.0 Probable transcription regulator Mig-14 compare
Pf6N2E2_3655 +8.7 67.5 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_3664 +8.2 32.3 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_4974 +8.1 54.7 DedA protein compare
Pf6N2E2_2513 +7.2 20.6 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_3908 +7.0 47.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_773 +6.6 43.1 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_2510 +6.6 14.7 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3661 +5.9 12.5 hypothetical protein compare
Pf6N2E2_4153 +5.2 12.5 Sensory box histidine kinase compare
Pf6N2E2_2508 +5.2 8.0 hypothetical protein compare
Pf6N2E2_5242 +5.2 13.3 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_2507 +5.1 15.0 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_3899 +5.1 30.2 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_2509 +5.0 9.0 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2518 +4.0 2.8 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_4451 +3.9 22.8 FIG139438: lipoprotein B compare
Pf6N2E2_3179 +3.9 2.1 FIG021952: putative membrane protein compare
Pf6N2E2_2506 +3.7 19.8 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2755 +3.6 6.1 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_5579 +3.5 20.1 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_4556 +3.5 13.8 FIG00953078: hypothetical protein compare
Pf6N2E2_4579 +3.5 6.7 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_2681 +3.4 20.3 3-phosphoglycerate kinase compare
Pf6N2E2_1056 +3.3 9.8 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_2526 +3.2 14.5 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_3791 +3.1 22.0 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_4063 +3.1 3.9 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf6N2E2_3792 +3.0 16.2 Glucans biosynthesis protein G precursor compare
Pf6N2E2_4654 +3.0 4.9 hypothetical protein compare
Pf6N2E2_5943 +2.9 1.9 hypothetical protein compare
Pf6N2E2_5520 +2.9 14.2 Sensory box histidine kinase compare
Pf6N2E2_2384 +2.8 11.6 CmpX compare
Pf6N2E2_3793 +2.8 4.6 D-tyrosyl-tRNA(Tyr) deacylase (EC 3.6.1.n1) compare
Pf6N2E2_4612 +2.6 13.7 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_4810 +2.5 12.5 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_817 +2.5 5.9 Protein-glutamate methylesterase (EC 3.1.1.61) compare
Pf6N2E2_4907 +2.4 16.2 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) compare
Pf6N2E2_445 +2.3 13.0 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_4889 +2.3 6.8 Putative transport protein compare
Pf6N2E2_3611 +2.3 4.2 HflC protein compare
Pf6N2E2_2277 +2.2 8.3 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_3198 +2.2 5.1 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_3612 +2.2 5.7 HflK protein compare
Pf6N2E2_4084 +2.2 11.7 Transcriptional regulator compare
Pf6N2E2_3847 +2.2 8.0 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_4418 +2.1 4.0 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_4148 +2.1 12.4 Glutamate-aspartate carrier protein compare
Pf6N2E2_3941 +2.1 7.0 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_4581 +2.1 5.6 FIG015547: peptidase, M16 family conserved
Pf6N2E2_5494 +2.0 10.3 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_5156 +2.0 5.6 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_6059 +1.9 5.7 FIG008443: hypothetical protein compare
Pf6N2E2_1031 +1.9 1.6 Alkanesulfonates transport system permease protein compare
Pf6N2E2_3429 +1.9 2.9 hypothetical protein compare
Pf6N2E2_2313 +1.9 6.5 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_4838 +1.9 13.6 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
Pf6N2E2_4159 +1.9 8.0 Cytochrome c4 compare
Pf6N2E2_5150 +1.8 10.0 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_5331 +1.8 9.6 ATPase, AFG1 family compare
Pf6N2E2_4464 +1.8 4.6 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_5001 +1.8 2.9 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_4614 +1.8 6.6 twitching motility protein PilH compare
Pf6N2E2_2320 +1.7 6.0 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_5525 +1.7 8.7 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases conserved
Pf6N2E2_2281 +1.7 3.8 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_5722 +1.7 4.5 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf6N2E2_4580 +1.7 5.7 FIG015287: Zinc protease compare
Pf6N2E2_4479 +1.7 0.9 FIG001590: Putative conserved exported protein precursor compare
Pf6N2E2_4809 +1.7 13.5 COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases compare
Pf6N2E2_5014 +1.6 3.0 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_3794 +1.6 5.9 Proline iminopeptidase (EC 3.4.11.5) compare
Pf6N2E2_4120 +1.6 10.5 Trk system potassium uptake protein TrkA compare
Pf6N2E2_5111 +1.6 3.4 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_5153 +1.6 4.0 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_4454 +1.6 8.2 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_4413 +1.6 5.2 FIG004064: hypothetical protein compare
Pf6N2E2_4803 +1.5 3.0 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_3826 +1.5 6.0 Nitrogen regulation protein NR(I) compare
Pf6N2E2_4658 +1.5 1.8 FIG00955006: hypothetical protein compare
Pf6N2E2_2592 +1.5 7.9 Flagellar motor switch protein FliG compare
Pf6N2E2_3412 +1.5 1.9 FIG006045: Sigma factor, ECF subfamily compare
Pf6N2E2_3641 +1.4 2.1 ADP-ribose pyrophosphatase (EC 3.6.1.13) compare
Pf6N2E2_3763 +1.4 1.5 hypothetical protein compare
Pf6N2E2_4276 +1.4 8.0 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_4432 +1.4 7.1 Uroporphyrinogen-III synthase (EC 4.2.1.75) compare
Pf6N2E2_5328 +1.4 6.0 FIG00958649: hypothetical protein compare
Pf6N2E2_2311 +1.4 8.8 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf6N2E2_3199 +1.3 8.7 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_2634 +1.3 7.9 Potassium uptake protein TrkH compare
Pf6N2E2_2310 +1.3 9.0 Cytochrome c oxidase subunit CcoN (EC 1.9.3.1) compare
Pf6N2E2_6057 +1.3 4.5 FIG002781: Alpha-L-glutamate ligase family protein compare
Pf6N2E2_5339 +1.3 6.1 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_2007 +1.3 2.1 Error-prone, lesion bypass DNA polymerase V (UmuC) compare
Pf6N2E2_3072 +1.3 4.9 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_4638 +1.3 3.3 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_2514 +1.3 1.5 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_1625 +1.3 3.7 Ferric reductase (1.6.99.14) compare
Pf6N2E2_2413 +1.3 1.9 FIG074102: hypothetical protein compare
Pf6N2E2_1751 +1.3 2.8 Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21) compare
Pf6N2E2_4121 +1.3 2.3 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_2597 +1.2 8.3 Flagellar regulatory protein FleQ compare
Pf6N2E2_2594 +1.2 7.5 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_1566 +1.2 1.3 FIG00960770: hypothetical protein compare
Pf6N2E2_5944 +1.2 5.2 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
Pf6N2E2_3539 +1.2 1.5 FIG00955840: hypothetical protein compare
Pf6N2E2_4673 +1.2 3.8 ATP-dependent 23S rRNA helicase DbpA compare
Pf6N2E2_942 +1.2 2.7 Mll6465 protein compare
Pf6N2E2_5771 +1.2 6.6 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_2985 +1.2 2.2 Diacylglycerol kinase (EC 2.7.1.107) compare
Pf6N2E2_2822 +1.1 7.0 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_4615 +1.1 1.6 type IV pili signal transduction protein PilI compare
Pf6N2E2_2576 +1.1 8.0 Flagellar biosynthesis protein FlhA compare
Pf6N2E2_2527 +1.1 4.4 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_2590 +1.1 7.1 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_4918 +1.1 1.8 Ribonucleotide reductase transcriptional regulator NrdR compare
Pf6N2E2_2213 +1.1 4.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2425 +1.1 2.6 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2318 +1.1 6.5 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_3606 +1.1 6.4 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-) compare
Pf6N2E2_4233 +1.1 9.3 sulfatase domain protein, putative compare
Pf6N2E2_3344 +1.1 2.8 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD compare
Pf6N2E2_2575 +1.1 6.2 Flagellar biosynthesis protein FlhF compare
Pf6N2E2_114 +1.1 1.3 Polysaccharide biosynthesis protein compare
Pf6N2E2_2449 +1.1 1.6 FIG074102: hypothetical protein compare
Pf6N2E2_2593 +1.1 5.2 Flagellar M-ring protein FliF compare
Pf6N2E2_923 +1.1 2.7 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_2872 +1.1 7.5 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_2580 +1.1 2.7 Flagellar biosynthesis protein FliP compare
Pf6N2E2_1984 +1.0 2.2 probable exported protein STY4558 compare
Pf6N2E2_2582 +1.0 3.2 Flagellar motor switch protein FliN compare
Pf6N2E2_1490 +1.0 1.9 hypothetical protein compare
Pf6N2E2_5574 +1.0 6.8 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_5158 +1.0 4.1 FIG00954153: hypothetical protein compare
Pf6N2E2_3461 +1.0 1.6 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_288 +1.0 2.1 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_5647 +1.0 5.0 Transcriptional regulator, TetR family compare
Pf6N2E2_5545 +1.0 4.4 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_2583 +1.0 4.5 Flagellar motor switch protein FliM compare
Pf6N2E2_3762 +1.0 4.5 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_2317 +1.0 3.0 Putative analog of CcoH, COG3198 compare
Pf6N2E2_757 +1.0 4.3 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) compare
Pf6N2E2_4618 +1.0 2.7 Chemotaxis signal transduction protein compare
Pf6N2E2_2264 +1.0 2.9 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf6N2E2_2010 +1.0 1.4 hypothetical protein compare
Pf6N2E2_5840 +1.0 1.2 hypothetical protein compare
Pf6N2E2_2591 +0.9 4.0 Flagellar assembly protein FliH compare
Pf6N2E2_4433 +0.9 5.9 Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives) compare
Pf6N2E2_4087 +0.9 4.9 L-asparaginase (EC 3.5.1.1) (from data) compare
Pf6N2E2_4915 +0.9 1.6 FIG000875: Thioredoxin domain-containing protein EC-YbbN compare
Pf6N2E2_339 +0.9 2.1 Glutathione-regulated potassium-efflux system protein KefC compare
Pf6N2E2_4630 +0.9 4.0 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf6N2E2_5877 +0.9 2.2 Phosphohistidine phosphatase SixA compare
Pf6N2E2_525 +0.9 3.9 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_1983 +0.9 2.0 hypothetical protein compare
Pf6N2E2_3475 +0.9 2.2 hypothetical protein compare
Pf6N2E2_3725 +0.9 1.6 FIG085779: Lipoprotein compare
Pf6N2E2_5508 +0.9 1.4 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_1987 +0.9 1.7 hypothetical protein compare
Pf6N2E2_4617 +0.9 7.1 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_6035 +0.9 2.8 alpha/beta hydrolase fold compare
Pf6N2E2_4307 +0.9 2.0 Type VI secretion lipoprotein/VasD compare
Pf6N2E2_5865 +0.9 6.4 Chaperone protein HtpG compare
Pf6N2E2_156 +0.9 2.2 Carbon storage regulator compare
Pf6N2E2_1298 +0.9 2.6 Xanthine transporter,putative compare
Pf6N2E2_6140 +0.9 1.3 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_2712 +0.9 4.0 Permeases of the major facilitator superfamily compare
Pf6N2E2_2285 +0.9 1.2 FIG00954079: hypothetical protein compare
Pf6N2E2_613 +0.9 1.5 lipoprotein, putative compare
Pf6N2E2_4086 +0.9 7.4 sodium/alanine transporter compare
Pf6N2E2_3955 +0.9 1.1 FIG00953394: hypothetical protein compare
Pf6N2E2_5745 +0.9 3.6 Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73) compare
Pf6N2E2_3761 +0.9 7.1 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_2309 +0.9 1.5 hypothetical protein compare
Pf6N2E2_4601 +0.9 2.0 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_2502 +0.9 3.1 O-antigen acetylase compare
Pf6N2E2_2579 +0.8 3.7 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_2578 +0.8 1.7 Flagellar biosynthesis protein FliR compare
Pf6N2E2_2563 +0.8 6.0 FIG00953562: hypothetical protein compare
Pf6N2E2_4987 +0.8 1.4 MaoC-like domain protein compare
Pf6N2E2_5816 +0.8 1.8 Smr domain protein compare
Pf6N2E2_4477 +0.8 3.0 Z-ring-associated protein ZapA compare
Pf6N2E2_1626 +0.8 3.8 Putative ATP-binding component of a transport system compare
Pf6N2E2_4659 +0.8 0.9 Lipoprotein, putative compare
Pf6N2E2_4054 +0.8 1.9 Osmotically inducible protein C compare
Pf6N2E2_315 +0.8 2.1 probable transmembrane protein compare
Pf6N2E2_5742 +0.8 3.6 Cobyric acid synthase (EC 6.3.5.10) compare
Pf6N2E2_5046 +0.8 4.2 ATP-dependent RNA helicase PA3950 compare
Pf6N2E2_2323 +0.8 2.3 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_5677 +0.8 1.6 Carbon storage regulator compare
Pf6N2E2_2754 +0.8 2.9 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf6N2E2_2577 +0.8 4.7 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_2436 +0.8 1.2 DNA-binding response regulator, LuxR family compare
Pf6N2E2_5155 +0.8 1.8 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_1215 +0.8 2.1 probable membrane protein YPO2362 compare
Pf6N2E2_2124 +0.8 1.0 Mg(2+) transport ATPase protein C compare
Pf6N2E2_2428 +0.8 1.5 Thiol-disulfide isomerase and thioredoxins compare


Specific Phenotypes

For 14 genes in this experiment

For stress Rifampicin in Pseudomonas fluorescens FW300-N2E2

For stress Rifampicin across organisms