Experiment set3IT061 for Pseudomonas fluorescens FW300-N2E2

LB with Rifampicin 0.003 mg/ml

Group: stress
Media: LB + Rifampicin (0.003 mg/ml) + Dimethyl Sulfoxide (0.064285714 vol%)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.4 generations
By: Mark on 3/14/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: plate1 A1

Specific Phenotypes

For 14 genes in this experiment

For stress Rifampicin in Pseudomonas fluorescens FW300-N2E2

For stress Rifampicin across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Aromatic amino acid degradation	1
Experimental tye	1
Heme and Siroheme Biosynthesis	1
Proline, 4-hydroxyproline uptake and utilization	1
YjeE	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Arginine and proline metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tetrapyrrole biosynthesis II (from glycine)	4	3	1
tetrapyrrole biosynthesis I (from glutamate)	6	6	1
superpathway of heme b biosynthesis from glycine	8	7	1
superpathway of heme b biosynthesis from glutamate	10	10	1
peptidoglycan recycling II	10	8	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1
adenosylcobalamin biosynthesis II (aerobic)	33	31	1
adenosylcobalamin biosynthesis I (anaerobic)	36	29	1