Experiment set3IT061 for Burkholderia phytofirmans PsJN

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L-Asparagine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Asparagine (20 mM), pH=7
Culturing: BFirm_ML3, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 5.7 generations
By: Mark on 1/27/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Plate 1 B2

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source L-Asparagine in Burkholderia phytofirmans PsJN

For nitrogen source L-Asparagine across organisms

SEED Subsystems

Subsystem #Specific
Methionine Biosynthesis 2
Biotin biosynthesis 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Lysine Biosynthesis DAP Pathway 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-asparagine degradation I 1 1 1
L-asparagine degradation III (mammalian) 3 2 1
L-methionine salvage from L-homocysteine 3 1 1
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 1
L-methionine biosynthesis III 4 4 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 2 1
L-methionine biosynthesis I 5 3 1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation 5 2 1
superpathway of L-homoserine and L-methionine biosynthesis 8 6 1
L-lysine biosynthesis I 9 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 2
folate transformations III (E. coli) 9 8 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
folate transformations II (plants) 11 9 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
aspartate superpathway 25 23 2
folate transformations I 13 8 1
biotin biosynthesis I 15 13 1
superpathway of L-methionine salvage and degradation 16 7 1