Experiment set3IT059 for Desulfovibrio vulgaris Miyazaki F

Compare to:

MoYLS4 with Bacitracin 1.4 mg/ml

Group: stress
Media: MoYLS4 + Bacitracin (1.4 mg/ml), pH=7.2
Culturing: Miya_ML3_a, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 11/31/2014
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 33 genes in this experiment

For stress Bacitracin in Desulfovibrio vulgaris Miyazaki F

For stress Bacitracin across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter oligopeptide (TC 3.A.1.5.1) 2
Peptidoglycan Biosynthesis 2
ABC transporter dipeptide (TC 3.A.1.5.2) 1
ATP-dependent RNA helicases, bacterial 1
Bacterial Cell Division 1
Biogenesis of c-type cytochromes 1
Capsular Polysaccharide (CPS) of Campylobacter 1
Flagellar motility 1
Flagellum 1
Oxidative stress 1
Periplasmic disulfide interchange 1
Sex pheromones in Enterococcus faecalis and other Firmicutes 1
Transport of Zinc 1
Universal GTPases 1
Zinc resistance 1
dTDP-rhamnose synthesis 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-glucose biosynthesis 2 2 1
pseudouridine degradation 2 1 1
sucrose degradation II (sucrose synthase) 5 3 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 3 2
stachyose degradation 7 2 1
sucrose biosynthesis II 8 6 1
sucrose biosynthesis I (from photosynthesis) 9 7 1
colanic acid building blocks biosynthesis 11 10 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
type I lipoteichoic acid biosynthesis (S. aureus) 17 5 1
superpathway of anaerobic sucrose degradation 19 15 1