Experiment set3IT057 for Pseudomonas fluorescens FW300-N2E3

LB with Sodium nitrate 200 mM

Group: stress
Media: LB + Sodium nitrate (200 mM)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 6/18/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 943 B3,B4

Specific Phenotypes

For 29 genes in this experiment

For stress Sodium nitrate in Pseudomonas fluorescens FW300-N2E3

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	2
Peptidoglycan Biosynthesis	2
ABC transporter dipeptide (TC 3.A.1.5.2)	1
Bacterial Cell Division	1
Bacterial Chemotaxis	1
Bacterial hemoglobins	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
Glycine and Serine Utilization	1
Glycine cleavage system	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1
Type IV pilus	1
Ubiquinone Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Glycine, serine and threonine metabolism
• Lysine degradation
• One carbon pool by folate
• Biotin metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-methyl-5'-thioadenosine degradation II	1	1	1
4-hydroxybenzoate biosynthesis II (bacteria)	1	1	1
adenine and adenosine salvage III	4	4	3
adenine and adenosine salvage V	3	2	2
adenine and adenosine salvage I	2	2	1
purine ribonucleosides degradation	6	5	3
adenosine nucleotides degradation II	5	5	2
glycine biosynthesis II	3	3	1
glycine degradation	3	3	1
glycine cleavage	3	3	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	3
polybrominated phenols biosynthesis	4	1	1
purine nucleotides degradation II (aerobic)	11	11	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	3
peptidoglycan biosynthesis II (staphylococci)	17	12	3
spongiadioxin C biosynthesis	7	2	1
polybrominated dihydroxylated diphenyl ethers biosynthesis	8	2	1
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
folate transformations III (E. coli)	9	9	1
photorespiration III	9	5	1
photorespiration I	9	5	1
reductive glycine pathway of autotrophic CO2 fixation	9	5	1
ubiquinol-8 biosynthesis (late decarboxylation)	9	4	1
p-HBAD biosynthesis	9	1	1
photorespiration II	10	6	1
nucleoside and nucleotide degradation (archaea)	10	2	1
L-methionine salvage cycle III	11	10	1
folate transformations II (plants)	11	10	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
superpathway of ubiquinol-8 biosynthesis (early decarboxylation)	12	10	1
folate transformations I	13	9	1
superpathway of purine nucleotide salvage	14	13	1
tetrahydromethanopterin biosynthesis	14	3	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
superpathway of polybrominated aromatic compound biosynthesis	20	2	1
phenolphthiocerol biosynthesis	23	1	1
superpathway of chorismate metabolism	59	43	1