Experiment set3IT055 for Pseudomonas fluorescens FW300-N2E2

LB with Ceftazidime hydrate 0.15 mg/ml

Group: stress
Media: LB + Ceftazidime hydrate (0.15 mg/ml)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1184 F5,F6

Specific Phenotypes

For 4 genes in this experiment

For stress Ceftazidime hydrate in Pseudomonas fluorescens FW300-N2E2

For stress Ceftazidime hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Beta-lactamase	1
Peptidoglycan Biosynthesis	1
Tn552	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Peptidoglycan biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1