Experiment set3IT054 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Doxycycline hyclate 0.0001 mg/ml

Group: stress
Media: LB + Doxycycline hyclate (1e-04 mg/ml)
Culturing: pseudo6_N2E2_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/9/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1184 E5,E6

Specific Phenotypes

For 26 genes in this experiment

For stress Doxycycline hyclate in Pseudomonas fluorescens FW300-N2E2

For stress Doxycycline hyclate across organisms

SEED Subsystems

Subsystem #Specific
Alginate metabolism 1
Arginine and Ornithine Degradation 1
Aromatic amino acid degradation 1
Carboxysome 1
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
DNA-replication 1
DNA repair, bacterial RecFOR pathway 1
De Novo Pyrimidine Synthesis 1
LMPTP YwlE cluster 1
Lipid A modifications 1
Mannose Metabolism 1
Methionine Biosynthesis 1
Methionine Degradation 1
Pentose phosphate pathway 1
Queuosine-Archaeosine Biosynthesis 1
Respiratory dehydrogenases 1 1
Staphylococcal pathogenicity islands SaPI 1
Transport of Zinc 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine nucleobases salvage I 1 1 1
3-dehydroquinate biosynthesis I 2 2 1
pyrimidine nucleobases salvage II 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
poly-β-1,6-N-acetyl-D-glucosamine biosynthesis 4 3 2
NADH to cytochrome bd oxidase electron transfer I 2 1 1
D-mannose degradation II 2 1 1
D-mannose degradation I 2 1 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
pentose phosphate pathway (oxidative branch) I 3 3 1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation 3 2 1
mannitol biosynthesis 3 1 1
superpathway of pyrimidine nucleobases salvage 4 4 1
GDP-mannose biosynthesis 4 4 1
aerobic respiration I (cytochrome c) 4 3 1
mannitol degradation II 4 2 1
1,5-anhydrofructose degradation 5 2 1
Fe(II) oxidation 6 3 1
NAD(P)/NADPH interconversion 6 3 1
chorismate biosynthesis I 7 7 1
β-(1,4)-mannan degradation 7 2 1
pentose phosphate pathway 8 8 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of pyrimidine ribonucleosides salvage 10 6 1
colanic acid building blocks biosynthesis 11 9 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis 14 7 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of chorismate metabolism 59 43 1