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Experiment set3IT054 for
Methanococcus maripaludis S2
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Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS01650
+0.7
1.3
sulfurtransferase TusA family protein
compare
MMP
_RS08930
+0.7
1.5
hypothetical protein
compare
MMP
_RS07345
+0.6
3.4
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS06975
+0.5
1.8
sulfide-dependent adenosine diphosphate thiazole synthase
compare
MMP
_RS08310
+0.5
2.1
TMEM165/GDT1 family protein
compare
MMP
_RS01570
+0.5
1.6
hypothetical protein
compare
MMP
_RS07120
+0.4
3.8
coenzyme F420 hydrogenase subunit alpha
compare
MMP
_RS00545
+0.4
2.9
XRE family transcriptional regulator
compare
MMP
_RS02605
+0.4
0.7
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS05315
+0.4
1.3
hypothetical protein
compare
MMP
_RS05970
+0.4
2.1
class II SORL domain-containing protein
compare
MMP
_RS07860
+0.4
0.6
hypothetical protein
compare
MMP
_RS03520
+0.4
0.8
YwbE family protein
compare
MMP
_RS06155
+0.4
1.8
DUF4186 domain-containing protein
compare
MMP
_RS08775
+0.4
2.3
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS03300
+0.4
0.6
TOBE domain-containing protein
compare
MMP
_RS02410
+0.4
1.6
ABC transporter substrate-binding protein
compare
MMP
_RS06540
+0.3
1.4
3-methyl-2-oxobutanoate dehydrogenase subunit VorB
compare
MMP
_RS02310
+0.3
1.3
RimK-like ATPgrasp N-terminal domain-containing protein
compare
MMP
_RS05985
+0.3
0.9
hypothetical protein
compare
MMP
_RS07130
+0.3
2.9
coenzyme F420 hydrogenase subunit gamma
compare
MMP
_RS07135
+0.3
2.5
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS00675
+0.3
0.6
nitroreductase family protein
compare
MMP
_RS03465
+0.3
0.7
adenine phosphoribosyltransferase
compare
MMP
_RS02045
+0.3
1.0
tRNA-Met
compare
MMP
_RS08835
+0.3
2.2
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS01435
+0.3
1.9
DMT family transporter
compare
MMP
_RS06005
+0.3
1.5
heavy-metal-associated domain-containing protein
compare
MMP
_RS03340
+0.3
0.5
ABC transporter ATP-binding protein
compare
MMP
_RS06930
+0.3
2.2
MBL fold metallo-hydrolase
compare
MMP
_RS07095
+0.3
1.1
PspC domain-containing protein
compare
MMP
_RS00285
+0.3
2.0
TIGR04013 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS04030
+0.3
1.8
hypothetical protein
compare
MMP
_RS02745
+0.3
1.1
hypothetical protein
compare
MMP
_RS08485
+0.3
2.0
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS07985
+0.3
2.5
nitroreductase family protein
compare
MMP
_RS09025
+0.3
0.7
hypothetical protein
compare
MMP
_RS08395
+0.3
2.2
DsrE family protein
compare
MMP
_RS05945
+0.3
1.7
CBS domain-containing protein
compare
MMP
_RS06910
+0.3
2.1
RlmF-related methyltransferase
compare
MMP
_RS02370
+0.3
1.5
NifB/NifX family molybdenum-iron cluster-binding protein
compare
MMP
_RS07895
+0.3
1.2
cytochrome c
compare
MMP
_RS00795
+0.3
2.1
formate dehydrogenase H subunit alpha selenocysteine-containing
compare
MMP
_RS08500
+0.3
1.9
pyridoxal 5'-phosphate synthase glutaminase subunit PdxT
compare
MMP
_RS07805
+0.3
1.6
ABC transporter permease
compare
MMP
_RS00665
+0.3
1.5
hypothetical protein
compare
MMP
_RS05355
+0.3
0.5
hypothetical protein
compare
MMP
_RS08315
+0.3
0.9
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMP
_RS05835
+0.3
0.4
secondary thiamine-phosphate synthase enzyme YjbQ
compare
MMP
_RS06610
+0.3
1.0
DUF2334 domain-containing protein
compare
MMP
_RS08580
+0.2
1.2
flagellin
compare
MMP
_RS07850
+0.2
1.4
prephenate dehydratase
compare
MMP
_RS08700
+0.2
2.1
hypothetical protein
compare
MMP
_RS06560
+0.2
2.1
hypothetical protein
compare
MMP
_RS07115
+0.2
1.4
MBL fold metallo-hydrolase
compare
MMP
_RS05975
+0.2
0.7
hypothetical protein
compare
MMP
_RS03065
+0.2
1.2
AzlC family ABC transporter permease
compare
MMP
_RS01630
+0.2
1.8
N-glycosylase/DNA lyase
compare
MMP
_RS04740
+0.2
1.5
DUF473 domain-containing protein
compare
MMP
_RS04205
+0.2
1.5
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS02770
+0.2
1.4
hypothetical protein
compare
MMP
_RS07560
+0.2
1.4
prefoldin subunit alpha
compare
MMP
_RS08075
+0.2
1.5
DUF749 domain-containing protein
compare
MMP
_RS03910
+0.2
0.9
hypothetical protein
compare
MMP
_RS07100
+0.2
1.9
hypothetical protein
compare
MMP
_RS05790
+0.2
1.3
HDIG domain-containing protein
compare
MMP
_RS01855
+0.2
1.7
2-hydroxyacyl-CoA dehydratase family protein
compare
MMP
_RS08260
+0.2
1.6
DUF99 family protein
compare
MMP
_RS01430
+0.2
1.5
mechanosensitive ion channel family protein
compare
MMP
_RS08980
+0.2
1.1
hypothetical protein
compare
MMP
_RS02790
+0.2
0.5
class III signal peptide-containing protein
compare
MMP
_RS06480
+0.2
1.2
ABC transporter substrate-binding protein
compare
MMP
_RS05715
+0.2
1.6
hypothetical protein
compare
MMP
_RS06710
+0.2
1.5
selenium-binding protein
compare
MMP
_RS07590
+0.2
1.8
hypothetical protein
compare
MMP
_RS06655
+0.2
1.5
glycoside hydrolase family 15 protein
compare
MMP
_RS08455
+0.2
1.2
multiprotein bridging factor aMBF1
compare
MMP
_RS08850
+0.2
1.9
hypothetical protein
compare
MMP
_RS08390
+0.2
1.4
DUF5400 domain-containing protein
compare
MMP
_RS08205
+0.2
1.6
stage II sporulation protein M
compare
MMP
_RS07835
+0.2
0.9
TldD/PmbA family protein
compare
MMP
_RS00660
+0.2
1.8
alkaline phosphatase family protein
compare
MMP
_RS03890
+0.2
1.3
DUF2540 domain-containing protein
compare
MMP
_RS05940
+0.2
1.5
corrinoid protein
compare
MMP
_RS08380
+0.2
1.5
phosphatase PAP2 family protein
compare
MMP
_RS01065
+0.2
1.7
alpha/beta hydrolase
compare
MMP
_RS07925
+0.2
1.4
haloacid dehalogenase
compare
MMP
_RS06535
+0.2
0.6
2-oxoacid:acceptor oxidoreductase family protein
compare
MMP
_RS07150
+0.2
0.9
MTH895/ArsE family thioredoxin-like protein
compare
MMP
_RS01775
+0.2
0.9
TIGR00297 family protein
compare
MMP
_RS03220
+0.2
0.6
RNA 2' 3'-cyclic phosphodiesterase
compare
MMP
_RS00480
+0.2
1.3
hypothetical protein
compare
MMP
_RS01620
+0.2
1.1
hypothetical protein
compare
MMP
_RS07970
+0.2
1.0
hypothetical protein
compare
MMP
_RS08325
+0.2
1.5
hypothetical protein
compare
MMP
_RS09050
+0.2
1.3
CAP domain-containing protein
compare
MMP
_RS00865
+0.2
1.1
SLC13 family permease
compare
MMP
_RS01120
+0.2
1.3
permease
compare
MMP
_RS06165
+0.2
1.5
ABC transporter permease
compare
MMP
_RS01090
+0.2
1.4
ABC transporter substrate-binding protein
compare
MMP
_RS01685
+0.2
0.9
indolepyruvate oxidoreductase subunit beta
compare
MMP
_RS07640
+0.2
1.1
mechanosensitive ion channel family protein
compare
MMP
_RS03765
+0.2
1.4
CopG family ribbon-helix-helix protein
compare
MMP
_RS08620
+0.2
1.1
flagellar accessory protein FlaH
compare
MMP
_RS07840
+0.2
0.9
ribonuclease III
compare
MMP
_RS08610
+0.2
1.4
flagellar protein F
compare
MMP
_RS05470
+0.2
1.3
DUF523 and DUF1722 domain-containing protein
compare
MMP
_RS07155
+0.2
1.4
MFS transporter
compare
MMP
_RS08275
+0.2
1.3
hypothetical protein
compare
MMP
_RS07770
+0.2
1.3
alanine racemase
compare
MMP
_RS04595
+0.2
0.9
hypothetical protein
compare
MMP
_RS08160
+0.2
1.2
EamA family transporter
compare
MMP
_RS03820
+0.2
1.1
EamA family transporter
compare
MMP
_RS08245
+0.2
1.2
DUF4013 domain-containing protein
compare
MMP
_RS09060
+0.2
1.4
hypothetical protein
compare
MMP
_RS08130
+0.2
1.2
IGHMBP2 family helicase
compare
MMP
_RS01980
+0.2
1.5
trimeric intracellular cation channel family protein
compare
MMP
_RS08405
+0.2
1.3
MFS transporter
compare
MMP
_RS00155
+0.2
1.3
heavy metal-binding domain-containing protein
compare
MMP
_RS08250
+0.2
1.3
pyruvate kinase
compare
MMP
_RS08175
+0.2
1.2
7-cyano-7-deazaguanine synthase
compare
MMP
_RS01945
+0.2
1.2
DUF4350 domain-containing protein
compare
MMP
_RS01635
+0.2
1.1
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS08575
+0.2
1.4
hypothetical protein
compare
MMP
_RS08625
+0.2
1.3
type II/IV secretion system ATPase subunit
compare
MMP
_RS05960
+0.2
1.2
CoB--CoM heterodisulfide reductase subunit B
compare
MMP
_RS01105
+0.2
1.1
hypothetical protein
compare
MMP
_RS00540
+0.1
1.2
PLP-dependent aminotransferase family protein
compare
MMP
_RS09035
+0.1
0.6
hypothetical protein
compare
MMP
_RS08630
+0.1
0.9
archaellar assembly protein FlaJ
compare
MMP
_RS05275
+0.1
1.2
AI-2E family transporter
compare
MMP
_RS06430
+0.1
0.9
6-carboxytetrahydropterin synthase QueD
compare
MMP
_RS03215
+0.1
1.2
MBL fold metallo-hydrolase
compare
MMP
_RS08415
+0.1
0.7
radical SAM protein
compare
MMP
_RS00630
+0.1
1.2
ECF transporter S component
compare
MMP
_RS01060
+0.1
1.2
calcium/sodium antiporter
compare
MMP
_RS08235
+0.1
0.9
hypothetical protein
compare
MMP
_RS03150
+0.1
1.1
phosphoglycerate mutase
compare
MMP
_RS05680
+0.1
1.0
phosphate signaling complex protein PhoU
compare
MMP
_RS03745
+0.1
1.1
phenylacetate--CoA ligase
compare
MMP
_RS00055
+0.1
1.1
DUF2254 domain-containing protein
compare
MMP
_RS03830
+0.1
1.2
exodeoxyribonuclease VII large subunit
compare
MMP
_RS08265
+0.1
0.8
rubrerythrin
compare
MMP
_RS04365
+0.1
1.2
M48 family metallopeptidase
compare
MMP
_RS02805
+0.1
0.4
HEPN domain-containing protein
compare
MMP
_RS05175
+0.1
0.7
hypothetical protein
compare
MMP
_RS07650
+0.1
1.2
TldD/PmbA family protein
compare
MMP
_RS06665
+0.1
1.0
glycogen/starch synthase
compare
MMP
_RS03790
+0.1
0.9
hypothetical protein
compare
MMP
_RS00945
+0.1
0.4
PadR family transcriptional regulator
compare
MMP
_RS06150
+0.1
1.1
hypothetical protein
compare
MMP
_RS05530
+0.1
1.1
signal peptide peptidase SppA
compare
MMP
_RS00565
+0.1
0.7
CBS domain-containing protein
compare
MMP
_RS06320
+0.1
1.0
hypothetical protein
compare
MMP
_RS06555
+0.1
0.7
cupin domain-containing protein
compare
MMP
_RS00690
+0.1
1.0
MBL fold metallo-hydrolase
compare
MMP
_RS07855
+0.1
1.0
class I SAM-dependent methyltransferase
compare
MMP
_RS08615
+0.1
0.5
flagellar protein G
compare
MMP
_RS01940
+0.1
0.8
MoxR family ATPase
compare
MMP
_RS01525
+0.1
0.5
nucleoside-diphosphate kinase
compare
MMP
_RS03320
+0.1
0.4
metal-dependent transcriptional regulator
compare
MMP
_RS04015
+0.1
0.5
hypothetical protein
compare
MMP
_RS01320
+0.1
0.6
hypothetical protein
compare
MMP
_RS00390
+0.1
1.0
ammonium transporter
compare
MMP
_RS07140
+0.1
1.1
TIGR00341 family protein
compare
MMP
_RS04060
+0.1
0.7
hypothetical protein
compare
MMP
_RS03450
+0.1
0.4
glycine synthesis protein GlyXS
(from data)
compare
MMP
_RS08750
+0.1
0.8
hypothetical protein
compare
MMP
_RS08400
+0.1
0.7
MJ0307 family thioredoxin
compare
MMP
_RS06965
+0.1
0.9
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS01580
+0.1
0.7
PQQ-binding-like beta-propeller repeat protein
compare
MMP
_RS09065
+0.1
0.9
hypothetical protein
compare
MMP
_RS01145
+0.1
1.0
ABC transporter ATP-binding protein
compare
MMP
_RS08430
+0.1
0.6
HAD family hydrolase
compare
MMP
_RS01975
+0.1
1.0
hypothetical protein
compare
MMP
_RS06520
+0.1
0.6
DUF1786 domain-containing protein
compare
MMP
_RS02735
+0.1
0.7
MBL fold metallo-hydrolase
compare
MMP
_RS02295
+0.1
0.9
TatD family hydrolase
compare
MMP
_RS08480
+0.1
1.0
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS07675
+0.1
1.1
S-layer protein
compare
MMP
_RS07145
+0.1
0.6
AmmeMemoRadiSam system protein B
compare
MMP
_RS08640
+0.1
1.0
TIM barrel protein
compare
MMP
_RS02845
+0.1
1.0
hypothetical protein
compare
MMP
_RS00385
+0.1
0.8
P-II family nitrogen regulator
compare
MMP
_RS06045
+0.1
0.9
DNA polymerase
compare
MMP
_RS06735
+0.1
0.7
class I SAM-dependent rRNA methyltransferase
compare
MMP
_RS06905
+0.1
0.5
hypothetical protein
compare
MMP
_RS06065
+0.1
1.0
iron ABC transporter substrate-binding protein
compare
MMP
_RS07185
+0.1
0.9
pyridoxal phosphate-dependent aminotransferase
compare
MMP
_RS04150
+0.1
0.8
MFS transporter
compare
MMP
_RS01965
+0.1
0.6
DUF1616 domain-containing protein
compare
MMP
_RS08225
+0.1
0.9
RimK family alpha-L-glutamate ligase
compare
MMP
_RS02400
+0.1
1.0
site-2 protease family protein
compare
MMP
_RS05235
+0.1
0.9
exodeoxyribonuclease III
compare
MMP
_RS03965
+0.1
0.5
hypothetical protein
compare
MMP
_RS01460
+0.1
0.4
hypothetical protein
compare
MMP
_RS08215
+0.1
0.6
DUF483 domain-containing protein
compare
MMP
_RS05995
+0.1
0.8
FprA family A-type flavoprotein
compare
MMP
_RS07550
+0.1
0.9
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS00655
+0.1
0.5
hypothetical protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t1 experiments