Experiment set3IT050 for Bacteroides thetaiotaomicron VPI-5482

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Hyaluronic acid sodium salt from Streptococcus equi

Group: carbon source
Media: Varel_Bryant_medium + Hyaluronic acid sodium salt from Streptococcus equi (5 mg/ml)
Culturing: Btheta_ML6, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Hualan on 17-Aug-17
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 30 genes in this experiment

For carbon source Hyaluronic acid sodium salt from Streptococcus equi in Bacteroides thetaiotaomicron VPI-5482

For carbon source Hyaluronic acid sodium salt from Streptococcus equi across organisms

SEED Subsystems

Subsystem #Specific
Lipid A-Ara4N pathway ( Polymyxin resistance ) 2
Choline Transport 1
Choline and Betaine Uptake and Betaine Biosynthesis 1
Colanic acid biosynthesis 1
Iron acquisition in Vibrio 1
Methicillin resistance in Staphylococci 1
N-linked Glycosylation in Bacteria 1
Respiratory Complex I 1
Teichoic and lipoteichoic acids biosynthesis 1
Teichuronic acid biosynthesis 1
Ton and Tol transport systems 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-galacturonate biosynthesis I (from UDP-D-glucuronate) 1 1 1
UDP-α-D-glucuronate biosynthesis (from UDP-glucose) 1 1 1
UDP-α-D-xylose biosynthesis 2 2 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 2
choline-O-sulfate degradation 3 1 1
UDP-sugars interconversion 9 4 2
Escherichia coli serotype O:15 O antigen biosynthesis 5 1 1
enterobacterial common antigen biosynthesis 5 1 1
Salmonella enterica serotype O:54 O antigen biosynthesis 5 1 1
Salmonella enterica serotype O:13 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:56 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:77/Salmonella enterica serotype O:6,14 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:149/Shigella boydii serotype O1 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:177 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:50 O antigen biosynthesis 6 1 1
Escherichia coli serotype O:71/Salmonella enterica serotype O:28ac O antigen biosynthesis 7 3 1
Escherichia coli serotype O:157/Salmonella enterica serotype O:30 O antigen biosynthesis 7 2 1
Salmonella enterica serotype O:39 O antigen biosynthesis 7 2 1
L-ascorbate biosynthesis V (euglena, D-galacturonate pathway) 7 2 1
Salmonella enterica serotype O:18 O antigen biosynthesis 7 2 1
Escherichia coli serotype O:111/Salmonella enterica serotype O:35 O antigen biosynthesis 7 1 1
Salmonella enterica serotype O:6,7 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:2 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:152 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:1B/Salmonella enterica serotype O:42 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:7 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:85/Salmonella enterica serotype O:17 O antigen biosynthesis 7 1 1
Escherichia coli serotype O:117 O antigen biosynthesis 8 3 1
Escherichia coli serotype O:107 O antigen biosynthesis 8 3 1
Escherichia coli serotype O:104 O antigen biosynthesis 8 3 1
Escherichia coli serotype O:55/Salmonella enterica serotype O:50 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:128 O antigen biosynthesis 8 2 1
Shigella boydii serotype 6 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:51/Salmonella enterica serotype O:57 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:21/Salmonella enterica serotype O:38 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:127 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:86 O antigen biosynthesis 8 2 1
Escherichia coli serotype O:49 O antigen biosynthesis 8 1 1
Escherichia coli serotype O:52 O antigen biosynthesis 8 1 1
Escherichia coli serotype O:169 O antigen biosynthesis 9 2 1
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
Escherichia coli serotype O:183/Shigella boydii serotype O:10 O antigen biosynthesis 9 2 1
Escherichia coli serotype O:8 O antigen biosynthesis 9 1 1
superpathway of enterobacterial common antigen biosynthesis 10 4 1
Escherichia coli serotype O:9a O antigen biosynthesis 10 1 1
Escherichia coli serotype O:9 O antigen biosynthesis 10 1 1
poly(3-O-β-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis 10 1 1
colanic acid building blocks biosynthesis 11 10 1
poly(glycerol phosphate) wall teichoic acid biosynthesis 11 2 1
poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis) 12 1 1
poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus) 14 1 1
H. pylori 26695 O-antigen biosynthesis 21 1 1