Experiment set3IT046 for Pseudomonas fluorescens SBW25-INTG

Sodium acetate carbon source

Group: carbon source
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + Sodium acetate (40 mM) + Ammonium chloride (10 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 13 genes in this experiment

For carbon source Sodium acetate in Pseudomonas fluorescens SBW25-INTG

For carbon source Sodium acetate across organisms

SEED Subsystems

Subsystem	#Specific
Experimental tye	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Heme and Siroheme Biosynthesis	1
RNA processing and degradation, bacterial	1
Terminal cytochrome C oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Ascorbate and aldarate metabolism
• Oxidative phosphorylation
• Puromycin biosynthesis
• Geraniol degradation
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
glycerophosphodiester degradation	2	2	1
phosphatidylcholine resynthesis via glycerophosphocholine	2	1	1
UDP-α-D-xylose biosynthesis	2	1	1
arsenite to oxygen electron transfer	2	1	1
polymyxin resistance	6	3	2
arsenite to oxygen electron transfer (via azurin)	3	1	1
glycerol and glycerophosphodiester degradation	4	4	1
heme b biosynthesis II (oxygen-independent)	4	3	1
aerobic respiration I (cytochrome c)	4	3	1
aerobic respiration II (cytochrome c) (yeast)	4	3	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	4	1
Fe(II) oxidation	6	3	1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic)	9	3	1
UDP-sugars interconversion	9	2	1
teichuronic acid biosynthesis (B. subtilis 168)	9	2	1
colanic acid building blocks biosynthesis	11	11	1
anandamide biosynthesis I	12	3	1
superpathway of bacteriochlorophyll a biosynthesis	26	5	1