Experiment set3IT041 for Pseudomonas fluorescens FW300-N1B4

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LB with Sodium nitrate 100 mM

Group: stress
Media: LB + Sodium nitrate (100 mM)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1042 E3,E4

Specific Phenotypes

For 13 genes in this experiment

For stress Sodium nitrate in Pseudomonas fluorescens FW300-N1B4

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem #Specific
Experimental tye 2
2-phosphoglycolate salvage 1
Biogenesis of c-type cytochromes 1
Campylobacter Iron Metabolism 1
Coenzyme B12 biosynthesis 1
Dissimilatory nitrite reductase 1
Glycolate, glyoxylate interconversions 1
Heme and Siroheme Biosynthesis 1
Periplasmic disulfide interchange 1
Photorespiration (oxidative C2 cycle) 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
siroheme biosynthesis 4 4 3
factor 430 biosynthesis 7 3 3
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 2 1
tRNA processing 10 10 2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) 15 13 3
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) 13 13 2
photorespiration III 9 6 1
photorespiration I 9 6 1
photorespiration II 10 7 1
adenosylcobalamin biosynthesis I (anaerobic) 36 29 3
adenosylcobalamin biosynthesis II (aerobic) 33 31 2