Experiment set3IT041 for Pseudomonas fluorescens FW300-N1B4

LB with Sodium nitrate 100 mM

Group: stress
Media: LB + Sodium nitrate (100 mM)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1042 E3,E4

Specific Phenotypes

For 14 genes in this experiment

For stress Sodium nitrate in Pseudomonas fluorescens FW300-N1B4

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
Experimental tye	2
2-phosphoglycolate salvage	1
Ammonia assimilation	1
Biogenesis of c-type cytochromes	1
Campylobacter Iron Metabolism	1
Coenzyme B12 biosynthesis	1
Dissimilatory nitrite reductase	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycolate, glyoxylate interconversions	1
Heme and Siroheme Biosynthesis	1
Periplasmic disulfide interchange	1
Photorespiration (oxidative C2 cycle)	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Glutamate metabolism
• Bisphenol A degradation
• Glycerophospholipid metabolism
• alpha-Linolenic acid metabolism
• Glyoxylate and dicarboxylate metabolism
• Benzoate degradation via CoA ligation
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Nitrogen metabolism
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
siroheme biosynthesis	4	4	3
ammonia assimilation cycle III	3	3	2
factor 430 biosynthesis	7	3	3
L-glutamate and L-glutamine biosynthesis	7	5	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
tRNA processing	10	10	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	13	3
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	13	2
L-citrulline biosynthesis	8	7	1
photorespiration I	9	6	1
photorespiration III	9	6	1
photorespiration II	10	7	1
adenosylcobalamin biosynthesis I (anaerobic)	36	29	3
superpathway of L-citrulline metabolism	12	9	1
adenosylcobalamin biosynthesis II (aerobic)	33	31	2