Experiment set3IT036 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with Nalidixic acid sodium salt 0.004 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_3248 +2.2 7.6 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf1N1B4_3005 +1.9 15.0 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf1N1B4_3006 +1.9 10.4 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf1N1B4_1461 +1.7 6.5 Protein fixF compare
Pf1N1B4_1464 +1.7 11.0 Glycosyl transferase compare
Pf1N1B4_733 +1.7 2.8 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_2352 +1.7 10.4 Glutathione synthetase (EC 6.3.2.3) compare
Pf1N1B4_1830 +1.6 10.9 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_1463 +1.6 10.0 hypothetical protein compare
Pf1N1B4_3415 +1.6 3.7 Transcriptional regulator, TetR family compare
Pf1N1B4_2850 +1.6 10.4 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf1N1B4_1475 +1.5 8.1 FIG00962376: hypothetical protein compare
Pf1N1B4_2380 +1.5 12.1 Cytochrome B561 compare
Pf1N1B4_1035 +1.5 6.1 Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C) compare
Pf1N1B4_2270 +1.4 7.0 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain compare
Pf1N1B4_65 +1.4 7.9 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf1N1B4_1069 +1.4 7.6 YrbA protein compare
Pf1N1B4_1554 +1.3 8.9 predicted FeS cluster maintenance protein (from data) compare
Pf1N1B4_2381 +1.3 11.0 Protein yceI precursor compare
Pf1N1B4_1632 +1.3 6.7 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf1N1B4_2401 +1.3 2.9 Lipoprotein, putative compare
Pf1N1B4_3656 +1.2 2.3 Eukaryotic-type low-affinity urea transporter compare
Pf1N1B4_2851 +1.2 2.2 hypothetical protein compare
Pf1N1B4_2382 +1.2 8.1 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_2724 +1.1 6.5 Transcriptional regulator, AsnC family compare
Pf1N1B4_5916 +1.1 3.5 Similar to non-heme chloroperoxidase, sll5080 homolog compare
Pf1N1B4_2545 +1.1 9.3 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf1N1B4_3703 +1.1 3.2 NLP/P60 family protein compare
Pf1N1B4_1456 +1.1 6.1 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_885 +1.1 4.0 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_1636 +1.1 1.9 FIG00954739: hypothetical protein compare
Pf1N1B4_2713 +1.1 2.8 FIG00955339: hypothetical protein compare
Pf1N1B4_5745 +1.0 1.8 FIG139928: Putative protease compare
Pf1N1B4_623 +1.0 2.1 Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10) compare
Pf1N1B4_3362 +1.0 4.4 Multidrug resistance protein B compare
Pf1N1B4_1457 +1.0 6.8 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_102 +1.0 1.3 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf1N1B4_901 +1.0 3.0 hypothetical protein compare
Pf1N1B4_3485 +1.0 2.0 hypothetical protein compare
Pf1N1B4_1181 +1.0 4.2 UPF0125 protein yfjF compare
Pf1N1B4_349 +1.0 3.7 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf1N1B4_4650 +1.0 3.4 Aspartate racemase (EC 5.1.1.13) compare
Pf1N1B4_3866 +1.0 1.8 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf1N1B4_5924 +1.0 6.7 hypothetical protein compare
Pf1N1B4_572 +0.9 7.6 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_127 +0.9 2.7 FIG032225: Transcriptional regulator, LysR family compare
Pf1N1B4_756 +0.9 2.0 hypothetical protein compare
Pf1N1B4_5078 +0.9 2.4 Transcriptional regulator PhnF compare
Pf1N1B4_2870 +0.9 1.5 Regulator of competence-specific genes compare
Pf1N1B4_2612 +0.9 4.0 Putative transport protein compare
Pf1N1B4_2144 +0.9 6.1 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf1N1B4_4801 +0.9 2.8 Polysaccharide deacetylase compare
Pf1N1B4_2793 +0.9 2.8 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf1N1B4_1003 +0.9 2.5 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_5659 +0.9 6.8 NfuA Fe-S protein maturation compare
Pf1N1B4_973 +0.9 8.2 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf1N1B4_1782 +0.9 2.4 hypothetical protein compare
Pf1N1B4_516 +0.9 2.8 Sensor histidine kinase compare
Pf1N1B4_731 +0.9 2.6 Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like compare
Pf1N1B4_2547 +0.9 2.1 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf1N1B4_4029 +0.9 2.8 NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3) compare
Pf1N1B4_5275 +0.9 1.7 hypothetical protein compare
Pf1N1B4_4835 +0.9 1.9 Carbon starvation protein A compare
Pf1N1B4_3119 +0.9 2.7 Holin compare
Pf1N1B4_4198 +0.9 2.5 4-hydroxybenzoate transporter compare
Pf1N1B4_3751 +0.9 1.3 FIG00953060: hypothetical protein compare
Pf1N1B4_81 +0.8 2.3 Putative sulfate permease compare
Pf1N1B4_2117 +0.8 6.9 Ribonuclease PH (EC 2.7.7.56) compare
Pf1N1B4_5568 +0.8 2.0 hypothetical protein compare
Pf1N1B4_1780 +0.8 1.7 FIG00961586: hypothetical protein compare
Pf1N1B4_972 +0.8 7.6 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf1N1B4_1294 +0.8 2.1 VgrG protein compare
Pf1N1B4_5820 +0.8 2.4 Transcriptional regulator, Cro/CI family compare
Pf1N1B4_3368 +0.8 1.7 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf1N1B4_5668 +0.8 1.6 Peptidase, U7 family compare
Pf1N1B4_980 +0.8 2.1 hypothetical protein compare
Pf1N1B4_26 +0.8 4.1 Excinuclease ABC subunit B compare
Pf1N1B4_4487 +0.8 1.8 FIG00955631: hypothetical protein compare
Pf1N1B4_971 +0.8 6.6 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf1N1B4_765 +0.8 2.1 Cys-tRNA(Pro) deacylase YbaK compare
Pf1N1B4_43 +0.8 4.1 hypothetical protein compare
Pf1N1B4_4473 +0.8 1.7 Homoserine/homoserine lactone efflux protein compare
Pf1N1B4_740 +0.8 1.4 Leucyl-tRNA synthetase compare
Pf1N1B4_1629 +0.8 2.7 FIG00958480: hypothetical protein compare
Pf1N1B4_5115 +0.8 2.4 sucrose ABC transporter, ATPase component (from data) compare
Pf1N1B4_4107 +0.8 1.5 Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog compare
Pf1N1B4_5957 +0.8 1.7 Arsenical resistance operon repressor compare
Pf1N1B4_4200 +0.8 2.8 PhaK-like protein compare
Pf1N1B4_1315 +0.8 4.5 IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) compare
Pf1N1B4_2417 +0.8 2.6 Betaine aldehyde dehydrogenase (EC 1.2.1.8) compare
Pf1N1B4_5619 +0.8 2.2 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_267 +0.8 1.8 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf1N1B4_1458 +0.7 4.4 Probable transcription regulator Mig-14 compare
Pf1N1B4_1545 +0.7 5.2 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf1N1B4_241 +0.7 1.1 Thiol-disulfide isomerase and thioredoxins compare
Pf1N1B4_4462 +0.7 1.8 LysR family transcriptional regulator YbhD compare
Pf1N1B4_5370 +0.7 1.1 hypothetical protein compare
Pf1N1B4_909 +0.7 2.0 Ketosteroid isomerase-related protein compare
Pf1N1B4_3954 +0.7 3.2 Hydrolase, carbon-nitrogen family compare
Pf1N1B4_4041 +0.7 2.9 NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3) compare
Pf1N1B4_3968 +0.7 2.1 Spermidine/putrescine-binding periplasmic protein compare
Pf1N1B4_4826 +0.7 1.6 glutamine synthetase family protein compare
Pf1N1B4_5294 +0.7 1.7 hypothetical protein compare
Pf1N1B4_348 +0.7 2.1 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf1N1B4_3152 +0.7 3.9 LysR family transcriptional regulator PA3398 compare
Pf1N1B4_401 +0.7 2.7 GCN5-related N-acetyltransferase (EC 2.3.1.57) compare
Pf1N1B4_304 +0.7 1.9 Deoxyribonuclease TatD compare
Pf1N1B4_1247 +0.7 2.1 transcriptional regulator, MerR family compare
Pf1N1B4_3824 +0.7 4.9 FIG137360: hypothetical protein compare
Pf1N1B4_1091 +0.7 2.9 FIG138315: Putative alpha helix protein compare
Pf1N1B4_5219 +0.7 1.8 tRNA-Gly-CCC compare
Pf1N1B4_3484 +0.7 3.6 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf1N1B4_969 +0.7 6.8 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf1N1B4_2987 +0.7 2.3 NAD-dependent formate dehydrogenase delta subunit compare
Pf1N1B4_573 +0.7 4.7 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_5604 +0.7 2.1 Permeases of the major facilitator superfamily compare
Pf1N1B4_5927 +0.7 6.4 Capsular polysaccharide biosynthesis protein compare
Pf1N1B4_3191 +0.7 1.6 FIG00953505: hypothetical protein compare
Pf1N1B4_5858 +0.7 2.7 carboxyphosphonoenolpyruvate phosphonomutase, putative compare
Pf1N1B4_1263 +0.7 2.0 Cu(I)-responsive transcriptional regulator compare
Pf1N1B4_3222 +0.7 1.2 Ferredoxin compare
Pf1N1B4_2712 +0.7 2.8 tRNA-i(6)A37 methylthiotransferase compare
Pf1N1B4_1422 +0.7 2.1 hypothetical protein compare
Pf1N1B4_2947 +0.7 2.9 Restriction endonuclease compare
Pf1N1B4_1118 +0.7 1.3 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_5926 +0.7 4.9 hypothetical protein compare
Pf1N1B4_343 +0.7 2.5 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) compare
Pf1N1B4_5769 +0.7 1.6 Heme d1 biosynthesis protein NirL compare
Pf1N1B4_3111 +0.7 1.3 tRNA pseudouridine 13 synthase (EC 4.2.1.-) compare
Pf1N1B4_2969 +0.7 5.6 ATPase, AFG1 family compare
Pf1N1B4_1831 +0.7 4.8 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_5777 +0.7 1.2 Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL compare
Pf1N1B4_4723 +0.7 2.1 UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide compare
Pf1N1B4_476 +0.7 1.8 ABC-type multidrug transport system, ATPase component compare
Pf1N1B4_3411 +0.7 2.0 FIG00959431: hypothetical protein compare
Pf1N1B4_2706 +0.7 1.8 FIG00954871: hypothetical protein compare
Pf1N1B4_6 +0.7 2.9 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf1N1B4_3638 +0.7 2.2 FIG00955434: hypothetical protein compare
Pf1N1B4_447 +0.7 5.9 FIG004453: protein YceG like compare
Pf1N1B4_1888 +0.6 2.9 Glutamate-aspartate carrier protein compare
Pf1N1B4_4594 +0.6 1.0 Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) compare
Pf1N1B4_4509 +0.6 2.1 Aldose 1-epimerase compare
Pf1N1B4_4975 +0.6 2.0 hypothetical protein compare
Pf1N1B4_4419 +0.6 1.4 Organosulfonate utilization protein SsuF compare
Pf1N1B4_2484 +0.6 2.0 hypothetical protein compare
Pf1N1B4_2955 +0.6 2.7 L-lysine permease compare
Pf1N1B4_5838 +0.6 1.5 FIG00956589: hypothetical protein compare
Pf1N1B4_3567 +0.6 4.1 Phenylalanine hydroxylase transcriptional activator PhhR compare
Pf1N1B4_3435 +0.6 1.5 ABC transporter for L-Arginine and L-Citrulline, ATPase component (from data) compare
Pf1N1B4_4417 +0.6 0.9 Transcriptional regulator compare
Pf1N1B4_4172 +0.6 1.6 hypothetical protein compare
Pf1N1B4_5365 +0.6 1.9 hypothetical protein compare
Pf1N1B4_4102 +0.6 1.2 Mannose-6-phosphate isomerase (EC 5.3.1.8) compare
Pf1N1B4_175 +0.6 1.3 sigma-54 dependent transcriptional regulator compare
Pf1N1B4_3331 +0.6 1.2 Glycine cleavage system transcriptional antiactivator GcvR compare
Pf1N1B4_3501 +0.6 3.5 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_3044 +0.6 2.0 Putative lipoprotein, specific for Pseudomonas, in cluster with COG2110 compare
Pf1N1B4_3853 +0.6 1.9 Xanthine dehydrogenase iron-sulfur subunit (EC 1.17.1.4) / Xanthine dehydrogenase, FAD binding subunit (EC 1.17.1.4) compare
Pf1N1B4_1668 +0.6 2.8 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_1135 +0.6 1.6 FIG00960055: hypothetical protein compare
Pf1N1B4_5507 +0.6 1.1 hypothetical protein compare
Pf1N1B4_3745 +0.6 1.8 Thiamin ABC transporter, transmembrane component compare
Pf1N1B4_2195 +0.6 2.1 Small-conductance mechanosensitive channel compare
Pf1N1B4_3975 +0.6 1.7 Transcriptional regulator, IclR family compare
Pf1N1B4_970 +0.6 4.5 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf1N1B4_4220 +0.6 1.5 Maebl compare
Pf1N1B4_4271 +0.6 1.5 Hydrogen cyanide synthase HcnC / Opine oxidase subunit B compare
Pf1N1B4_1122 +0.6 4.6 Outer membrane porin, OprD family compare
Pf1N1B4_3328 +0.6 1.3 Rhodanese-like domain protein compare
Pf1N1B4_1667 +0.6 1.7 Histone acetyltransferase HPA2 and related acetyltransferases compare
Pf1N1B4_1500 +0.6 2.2 Chromosome (plasmid) partitioning protein ParA compare
Pf1N1B4_4699 +0.6 1.9 Multidrug translocase MdfA compare
Pf1N1B4_4035 +0.6 1.3 NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3) compare
Pf1N1B4_4592 +0.6 1.1 Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1) compare
Pf1N1B4_4040 +0.6 2.0 NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3) compare
Pf1N1B4_4355 +0.6 1.1 Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-) compare
Pf1N1B4_5801 +0.6 1.5 hypothetical protein compare
Pf1N1B4_2549 +0.6 3.7 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_1347 +0.6 1.4 Urea ABC transporter, ATPase protein UrtE compare
Pf1N1B4_2879 +0.6 3.5 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_2143 +0.6 4.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_3498 +0.6 1.7 FIG00953240: hypothetical protein compare
Pf1N1B4_4345 +0.6 1.9 hypothetical protein compare
Pf1N1B4_2719 +0.6 4.4 Sensor histidine kinase compare
Pf1N1B4_3347 +0.6 3.4 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf1N1B4_758 +0.6 1.9 Bacterioferritin compare
Pf1N1B4_221 +0.6 2.5 Citronellol and citronellal dehydrogenase compare
Pf1N1B4_1242 +0.6 1.9 FIG000605: protein co-occurring with transport systems (COG1739) compare
Pf1N1B4_4159 +0.6 1.9 FIG00955149: hypothetical protein compare
Pf1N1B4_3684 +0.6 2.8 Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) compare
Pf1N1B4_495 +0.6 2.0 Flp pilus assembly protein TadB compare
Pf1N1B4_71 +0.6 1.9 Glucarate dehydratase (EC 4.2.1.40) compare
Pf1N1B4_3965 +0.6 1.3 FIG033889: YebC paralog in Betaproteobacteria compare
Pf1N1B4_718 +0.6 3.2 outer membrane component of uptake system, probably for ferrous iron (from data) compare
Pf1N1B4_1411 +0.5 2.2 GTP-binding protein HflX compare
Pf1N1B4_683 +0.5 1.9 Fumarate hydratase class I, aerobic (EC 4.2.1.2) compare
Pf1N1B4_5509 +0.5 1.5 hypothetical protein compare
Pf1N1B4_218 +0.5 3.2 Periplasmic binding protein compare
Pf1N1B4_1426 +0.5 2.1 hypothetical protein compare
Pf1N1B4_2050 +0.5 3.7 Rubredoxin-NAD(+) reductase (EC 1.18.1.1) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Nalidixic acid sodium salt in Pseudomonas fluorescens FW300-N1B4

For stress Nalidixic acid sodium salt across organisms