Experiment set3IT035 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with Carbenicillin disodium salt 2 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_3248 +3.1 10.9 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf1N1B4_1095 +2.8 22.3 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf1N1B4_3484 +2.8 11.4 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf1N1B4_3166 +2.8 19.0 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_1888 +2.7 12.1 Glutamate-aspartate carrier protein compare
Pf1N1B4_2850 +2.7 16.7 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf1N1B4_3006 +2.6 15.4 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf1N1B4_262 +2.6 22.4 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf1N1B4_3415 +2.5 6.8 Transcriptional regulator, TetR family compare
Pf1N1B4_3005 +2.4 17.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf1N1B4_2917 +2.4 16.6 FIG140336: TPR domain protein compare
Pf1N1B4_388 +2.3 12.0 OmpA family protein compare
Pf1N1B4_733 +2.3 4.8 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_389 +2.3 13.6 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_4699 +2.3 3.4 Multidrug translocase MdfA compare
Pf1N1B4_1403 +2.3 14.1 3'-to-5' exoribonuclease RNase R compare
Pf1N1B4_1619 +2.2 16.5 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf1N1B4_2856 +2.2 11.4 Poly(A) polymerase (EC 2.7.7.19) compare
Pf1N1B4_1475 +2.2 12.1 FIG00962376: hypothetical protein compare
Pf1N1B4_1461 +2.1 7.8 Protein fixF compare
Pf1N1B4_5924 +2.0 9.3 hypothetical protein compare
Pf1N1B4_2724 +1.9 10.2 Transcriptional regulator, AsnC family compare
Pf1N1B4_65 +1.9 9.2 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf1N1B4_2635 +1.9 5.2 hypothetical protein compare
Pf1N1B4_339 +1.8 4.7 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf1N1B4_2026 +1.8 5.9 Transcriptional regulator compare
Pf1N1B4_1456 +1.8 9.9 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_1457 +1.7 11.4 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf1N1B4_2131 +1.7 5.9 hypothetical protein compare
Pf1N1B4_1465 +1.7 11.9 Carbamoyltransferase in large core OS assembly cluster compare
Pf1N1B4_1668 +1.7 10.3 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_3815 +1.6 6.6 FIG002781: Alpha-L-glutamate ligase family protein compare
Pf1N1B4_2036 +1.6 4.7 Phosphate transport system permease protein PstA (TC 3.A.1.7.1) compare
Pf1N1B4_4723 +1.6 4.7 UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide compare
Pf1N1B4_2436 +1.6 8.5 FIG00953563: hypothetical protein compare
Pf1N1B4_2853 +1.5 2.9 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_2878 +1.5 10.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_572 +1.5 10.8 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_3906 +1.4 5.4 DNA topoisomerase I (EC 5.99.1.2) compare
Pf1N1B4_4473 +1.4 2.9 Homoserine/homoserine lactone efflux protein compare
Pf1N1B4_1180 +1.4 2.9 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf1N1B4_2879 +1.4 8.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_1544 +1.4 7.9 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf1N1B4_573 +1.4 9.6 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_901 +1.4 4.8 hypothetical protein compare
Pf1N1B4_1830 +1.4 9.0 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_2736 +1.3 4.2 ATP-dependent RNA helicase PA3950 compare
Pf1N1B4_1466 +1.3 2.5 hypothetical protein compare
Pf1N1B4_5508 +1.3 3.7 hypothetical protein compare
Pf1N1B4_5745 +1.2 3.2 FIG139928: Putative protease compare
Pf1N1B4_3613 +1.2 2.2 hypothetical protein compare
Pf1N1B4_4835 +1.2 2.4 Carbon starvation protein A compare
Pf1N1B4_4560 +1.1 2.1 Transcriptional regulator, MerR family compare
Pf1N1B4_4028 +1.1 3.6 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) compare
Pf1N1B4_3817 +1.1 3.3 FIG008443: hypothetical protein compare
Pf1N1B4_3887 +1.1 3.0 CAAX amino terminal protease family protein compare
Pf1N1B4_5619 +1.1 2.2 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf1N1B4_4029 +1.1 3.7 NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3) compare
Pf1N1B4_2858 +1.1 8.4 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf1N1B4_547 +1.1 3.0 3-phosphoglycerate kinase compare
Pf1N1B4_1891 +1.1 3.2 ABC-type transport system involved in resistance to organic solvents, periplasmic component USSDB6C compare
Pf1N1B4_1630 +1.1 3.3 A/G-specific adenine glycosylase (EC 3.2.2.-) compare
Pf1N1B4_1887 +1.1 2.9 probable exported protein YPO0432 compare
Pf1N1B4_3169 +1.1 4.0 methylated-DNA--protein-cysteine methyltransferase-related protein compare
Pf1N1B4_1870 +1.0 3.2 Zinc ABC transporter, ATP-binding protein ZnuC compare
Pf1N1B4_3508 +1.0 4.2 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf1N1B4_3111 +1.0 2.1 tRNA pseudouridine 13 synthase (EC 4.2.1.-) compare
Pf1N1B4_814 +1.0 1.7 Transcriptional regulator, PadR family compare
Pf1N1B4_1008 +1.0 2.2 FIG006045: Sigma factor, ECF subfamily compare
Pf1N1B4_1096 +1.0 3.7 Septum formation protein Maf compare
Pf1N1B4_2218 +1.0 5.7 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf1N1B4_3443 +1.0 2.0 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf1N1B4_1135 +1.0 2.7 FIG00960055: hypothetical protein compare
Pf1N1B4_3816 +1.0 5.1 FIG139976: hypothetical protein compare
Pf1N1B4_327 +1.0 2.0 hypothetical protein compare
Pf1N1B4_2793 +1.0 3.3 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf1N1B4_5507 +1.0 1.9 hypothetical protein compare
Pf1N1B4_4537 +1.0 2.5 Uncharacterized protein ImpC compare
Pf1N1B4_5939 +1.0 3.8 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) compare
Pf1N1B4_1057 +1.0 2.7 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf1N1B4_2713 +1.0 2.5 FIG00955339: hypothetical protein compare
Pf1N1B4_868 +1.0 5.5 tRNA:Cm32/Um32 methyltransferase compare
Pf1N1B4_1464 +1.0 6.0 Glycosyl transferase compare
Pf1N1B4_4857 +1.0 3.4 Chemotaxis protein methyltransferase CheR (EC 2.1.1.80) compare
Pf1N1B4_3073 +1.0 5.1 probable glucosyl transferase compare
Pf1N1B4_4271 +1.0 2.1 Hydrogen cyanide synthase HcnC / Opine oxidase subunit B compare
Pf1N1B4_419 +1.0 2.0 Transcriptional regulator, TetR family compare
Pf1N1B4_6024 +1.0 3.7 FIG00953292: hypothetical protein compare
Pf1N1B4_2487 +1.0 5.5 Biotin synthase (EC 2.8.1.6) compare
Pf1N1B4_5779 +1.0 1.9 Nitrous oxide reductase maturation protein NosF (ATPase) compare
Pf1N1B4_407 +0.9 1.9 ADP-ribosylglycohydrolase YegU (EC 3.2.-.-) compare
Pf1N1B4_3195 +0.9 5.7 hydrolase, putative compare
Pf1N1B4_4866 +0.9 2.3 hypothetical protein compare
Pf1N1B4_1570 +0.9 4.3 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf1N1B4_650 +0.9 3.6 FIG001943: hypothetical protein YajQ compare
Pf1N1B4_5791 +0.9 2.3 hypothetical protein compare
Pf1N1B4_314 +0.9 2.9 Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1) compare
Pf1N1B4_2488 +0.9 5.0 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf1N1B4_5224 +0.9 2.6 tRNA-Arg-CCT compare
Pf1N1B4_672 +0.9 2.5 FIG00955538: hypothetical protein compare
Pf1N1B4_5292 +0.9 2.3 hypothetical protein compare
Pf1N1B4_3800 +0.9 2.4 Enoyl-CoA hydratase/isomerase family protein compare
Pf1N1B4_2622 +0.9 3.7 FIG006163: hypothetical protein compare
Pf1N1B4_1886 +0.9 3.0 FIG00956005: hypothetical protein compare
Pf1N1B4_3487 +0.9 4.2 Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate compare
Pf1N1B4_1871 +0.9 4.6 Zinc uptake regulation protein ZUR compare
Pf1N1B4_1679 +0.9 1.6 Membrane-fusion protein compare
Pf1N1B4_1460 +0.9 5.6 Lipid A core - O-antigen ligase and related enzymes compare
Pf1N1B4_4295 +0.9 2.8 FIG00954434: hypothetical protein compare
Pf1N1B4_2489 +0.9 3.5 Biotin synthesis protein BioH compare
Pf1N1B4_2891 +0.9 3.2 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf1N1B4_2854 +0.9 3.4 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf1N1B4_4831 +0.9 1.8 Outer membrane protein assembly factor YaeT precursor compare
Pf1N1B4_724 +0.9 2.5 DNA-binding response regulator ColR compare
Pf1N1B4_5863 +0.9 2.6 Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE compare
Pf1N1B4_1150 +0.9 3.6 AmpE protein compare
Pf1N1B4_2731 +0.9 2.7 Uncharacterized protein conserved in bacteria compare
Pf1N1B4_2023 +0.9 4.9 L-asparaginase (EC 3.5.1.1) (from data) compare
Pf1N1B4_1333 +0.8 3.6 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf1N1B4_2680 +0.8 6.4 MORN repeat family protein compare
Pf1N1B4_5242 +0.8 1.9 hypothetical protein compare
Pf1N1B4_5266 +0.8 1.5 hypothetical protein compare
Pf1N1B4_3501 +0.8 3.7 BarA sensory histidine kinase (= VarS = GacS) compare
Pf1N1B4_1153 +0.8 2.8 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf1N1B4_5041 +0.8 3.2 Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4) compare
Pf1N1B4_5584 +0.8 1.6 Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3) compare
Pf1N1B4_1159 +0.8 2.6 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf1N1B4_4801 +0.8 2.2 Polysaccharide deacetylase compare
Pf1N1B4_2886 +0.8 6.3 Paraquat-inducible protein B compare
Pf1N1B4_731 +0.8 2.6 Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like compare
Pf1N1B4_4951 +0.8 1.7 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf1N1B4_72 +0.8 1.6 Transcriptional regulator, LysR family compare
Pf1N1B4_3703 +0.8 2.2 NLP/P60 family protein compare
Pf1N1B4_2947 +0.8 3.7 Restriction endonuclease compare
Pf1N1B4_3812 +0.8 5.4 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10) compare
Pf1N1B4_3141 +0.8 2.0 FIG00959768: hypothetical protein compare
Pf1N1B4_5443 +0.8 2.7 hypothetical protein compare
Pf1N1B4_1348 +0.8 2.6 Urease accessory protein UreD compare
Pf1N1B4_507 +0.8 1.4 FIG00956448: hypothetical protein compare
Pf1N1B4_2130 +0.8 3.8 FIG00953403: hypothetical protein compare
Pf1N1B4_1724 +0.8 3.2 Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) compare
Pf1N1B4_3316 +0.8 2.1 Tricarboxylate transport transcriptional regulator TctD compare
Pf1N1B4_1399 +0.8 2.9 FIG003573: hypothetical protein compare
Pf1N1B4_1584 +0.8 2.2 Histidine ABC transporter, permease protein (TC 3.A.1) compare
Pf1N1B4_749 +0.8 1.7 hypothetical protein compare
Pf1N1B4_349 +0.8 2.6 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf1N1B4_920 +0.8 4.8 ATP-dependent RNA helicase RhlB compare
Pf1N1B4_273 +0.8 1.7 putative membrane protein compare
Pf1N1B4_13 +0.8 2.3 SAM-dependent methyltransferase YafE (UbiE paralog) compare
Pf1N1B4_2377 +0.8 4.0 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf1N1B4_2491 +0.8 2.8 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf1N1B4_1571 +0.8 3.5 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf1N1B4_2987 +0.8 3.1 NAD-dependent formate dehydrogenase delta subunit compare
Pf1N1B4_2859 +0.8 6.1 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf1N1B4_197 +0.8 1.4 FIG139991: Putative thiamine pyrophosphate-requiring enzyme compare
Pf1N1B4_5187 +0.7 1.3 5S RNA compare
Pf1N1B4_1872 +0.7 4.6 Zinc ABC transporter, periplasmic-binding protein ZnuA compare
Pf1N1B4_2493 +0.7 2.2 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf1N1B4_1344 +0.7 2.9 Urea ABC transporter, permease protein UrtB compare
Pf1N1B4_5661 +0.7 4.5 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) compare
Pf1N1B4_3507 +0.7 5.6 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf1N1B4_3458 +0.7 2.4 hypothetical protein compare
Pf1N1B4_5711 +0.7 2.4 Salicylate hydroxylase (EC 1.14.13.1) compare
Pf1N1B4_5937 +0.7 2.0 dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13) compare
Pf1N1B4_5302 +0.7 1.5 hypothetical protein compare
Pf1N1B4_3627 +0.7 1.3 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf1N1B4_3802 +0.7 1.4 PlcB, ORFX, ORFP, ORFB, ORFA, ldh gene compare
Pf1N1B4_5267 +0.7 2.1 hypothetical protein compare
Pf1N1B4_4030 +0.7 1.6 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) compare
Pf1N1B4_5812 +0.7 1.4 FIG00955248: hypothetical protein compare
Pf1N1B4_1572 +0.7 4.9 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf1N1B4_3325 +0.7 1.2 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf1N1B4_81 +0.7 1.2 Putative sulfate permease compare
Pf1N1B4_4355 +0.7 1.7 Gamma-glutamyl-aminobutyraldehyde dehydrogenase (EC 1.2.1.-) compare
Pf1N1B4_315 +0.7 4.2 Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1) compare
Pf1N1B4_4602 +0.7 1.7 Transcriptional regulators, LysR family compare
Pf1N1B4_5624 +0.7 1.6 Lipase precursor (EC 3.1.1.3) compare
Pf1N1B4_3895 +0.7 2.4 hypothetical protein compare
Pf1N1B4_5985 +0.7 2.2 FIG002465: BNR repeat protein compare
Pf1N1B4_2549 +0.7 4.3 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_2958 +0.7 1.9 Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-) compare
Pf1N1B4_5113 +0.7 2.5 sucrose ABC transporter, permease component 1 (from data) compare
Pf1N1B4_1091 +0.7 3.1 FIG138315: Putative alpha helix protein compare
Pf1N1B4_3730 +0.7 2.1 Sodium-dependent transporter compare
Pf1N1B4_3506 +0.7 3.6 Cysteine synthase B (EC 2.5.1.47) compare
Pf1N1B4_5644 +0.7 2.3 amino acid ABC transporter, permease protein compare
Pf1N1B4_3229 +0.7 3.9 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_646 +0.7 3.9 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases compare
Pf1N1B4_5589 +0.7 1.8 3-ketoacyl-CoA thiolase (EC 2.3.1.16) compare
Pf1N1B4_2415 +0.7 2.2 TldD family protein, Beta/Gamma-proteobacterial subgroup compare
Pf1N1B4_886 +0.7 1.8 FIG137478: Hypothetical protein compare
Pf1N1B4_4682 +0.7 2.5 Glycogen debranching enzyme (EC 3.2.1.-) compare
Pf1N1B4_4814 +0.7 1.8 Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1) compare
Pf1N1B4_2303 +0.7 5.3 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf1N1B4_4417 +0.7 1.6 Transcriptional regulator compare
Pf1N1B4_2637 +0.7 3.2 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) compare
Pf1N1B4_4019 +0.7 2.0 Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family compare
Pf1N1B4_4605 +0.7 2.0 FIG00953564: hypothetical protein compare
Pf1N1B4_4198 +0.7 2.0 4-hydroxybenzoate transporter compare
Pf1N1B4_1542 +0.7 1.6 Type IV pilus biogenesis protein PilN compare


Specific Phenotypes

For 18 genes in this experiment

For stress Carbenicillin disodium salt in Pseudomonas fluorescens FW300-N1B4

For stress Carbenicillin disodium salt across organisms