Experiment set3IT035 for Pseudomonas fluorescens FW300-N1B4

LB with Carbenicillin disodium salt 2 mg/ml

Group: stress
Media: LB + Carbenicillin disodium salt (2 mg/ml)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Adam on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1042 A7,A8

Specific Phenotypes

For 18 genes in this experiment

For stress Carbenicillin disodium salt in Pseudomonas fluorescens FW300-N1B4

For stress Carbenicillin disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	3
Beta-lactamase	1
DNA Repair Base Excision	1
DNA repair, UvrABC system	1
Peptidoglycan Biosynthesis	1
Tn552	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Histidine metabolism
• Glycosaminoglycan degradation
• Peptidoglycan biosynthesis
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
queuosine biosynthesis I (de novo)	4	4	1
L-histidine degradation I	4	4	1
L-histidine degradation II	5	5	1
queuosine biosynthesis III (queuosine salvage)	5	3	1
L-histidine degradation III	6	4	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
L-histidine degradation VI	8	7	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1