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Experiment set3IT035 for
Methanococcus maripaludis JJ
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formate McAA biotin dropout
200 most detrimental genes:
gene
name
fitness
t score
description
MMJJ
_RS04415
+2.3
10.2
hypothetical protein
compare
MMJJ
_RS08830
+1.0
3.5
hypothetical protein
compare
MMJJ
_RS04005
+0.9
7.2
acetate--CoA ligase family protein
compare
MMJJ
_RS08130
+0.9
1.0
translation initiation factor IF-2 subunit gamma
compare
MMJJ
_RS03725
+0.8
2.6
sulfurtransferase TusA family protein
compare
MMJJ
_RS07935
+0.6
0.7
protein fwdD
compare
MMJJ
_RS04340
+0.5
1.8
hypothetical protein
compare
MMJJ
_RS08390
+0.5
1.0
carboxymuconolactone decarboxylase family protein
compare
MMJJ
_RS07795
+0.5
2.3
cupin domain-containing protein
compare
MMJJ
_RS05685
+0.5
3.2
class III signal peptide-containing protein
compare
MMJJ
_RS07240
+0.5
4.3
MBL fold metallo-hydrolase
compare
MMJJ
_RS07025
+0.5
2.3
tRNA (cytidine(56)-2'-O)-methyltransferase
compare
MMJJ
_RS01100
+0.5
1.1
helix-turn-helix domain-containing protein
compare
MMJJ
_RS07235
+0.5
4.1
coenzyme F420 hydrogenase subunit alpha
compare
MMJJ
_RS06090
+0.5
3.0
hypothetical protein
compare
MMJJ
_RS07205
+0.5
2.6
MTH895/ArsE family thioredoxin-like protein
compare
MMJJ
_RS04350
+0.5
2.3
hypothetical protein
compare
MMJJ
_RS06675
+0.5
3.4
TIGR01212 family radical SAM protein
compare
MMJJ
_RS09405
+0.5
1.4
hypothetical protein
compare
MMJJ
_RS08485
+0.5
1.8
Lrp/AsnC family transcriptional regulator
compare
MMJJ
_RS05970
+0.4
1.3
MJ0307 family thioredoxin
compare
MMJJ
_RS09365
+0.4
1.9
hypothetical protein
compare
MMJJ
_RS08520
+0.4
1.4
peptidylprolyl isomerase
compare
MMJJ
_RS03835
+0.4
2.7
DUF504 domain-containing protein
compare
MMJJ
_RS07220
+0.4
3.3
coenzyme F420 hydrogenase subunit beta
compare
MMJJ
_RS03905
+0.4
2.1
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS04955
+0.4
2.2
tRNA pseudouridine(54/55) synthase Pus10
compare
MMJJ
_RS06190
+0.4
3.3
7-cyano-7-deazaguanine synthase
compare
MMJJ
_RS06385
+0.4
1.9
hypothetical protein
compare
MMJJ
_RS06515
+0.4
3.0
ribonuclease III
compare
MMJJ
_RS06495
+0.4
1.6
hypothetical protein
compare
MMJJ
_RS07185
+0.4
1.3
DUF371 domain-containing protein
compare
MMJJ
_RS01855
+0.4
2.8
MBL fold metallo-hydrolase
compare
MMJJ
_RS06640
+0.4
2.6
YfcE family phosphodiesterase
compare
MMJJ
_RS06440
+0.4
2.3
DUF4870 domain-containing protein
compare
MMJJ
_RS04480
+0.4
1.1
hypothetical protein
compare
MMJJ
_RS05915
+0.3
1.3
multiprotein bridging factor aMBF1
compare
MMJJ
_RS06745
+0.3
2.0
energy-coupling factor ABC transporter permease
compare
MMJJ
_RS01140
+0.3
2.1
helix-turn-helix domain-containing protein
compare
MMJJ
_RS06505
+0.3
1.2
prephenate dehydratase
compare
MMJJ
_RS07920
+0.3
2.3
6-carboxytetrahydropterin synthase QueD
compare
MMJJ
_RS06540
+0.3
1.3
DUF2120 domain-containing protein
compare
MMJJ
_RS07970
+0.3
0.5
O-phospho-L-seryl-tRNA:Cys-tRNA synthase
compare
MMJJ
_RS06460
+0.3
2.2
cytochrome c
compare
MMJJ
_RS00150
+0.3
2.0
DUF2180 family protein
compare
MMJJ
_RS06680
+0.3
1.8
DUF366 family protein
compare
MMJJ
_RS08305
+0.3
1.7
peroxiredoxin
compare
MMJJ
_RS05525
+0.3
2.0
GTP-binding protein
compare
MMJJ
_RS01010
+0.3
3.0
right-handed parallel beta-helix repeat-containing protein
compare
MMJJ
_RS00500
+0.3
1.3
GNAT family N-acetyltransferase
compare
MMJJ
_RS06470
+0.3
2.4
tripartite tricarboxylate transporter permease
compare
MMJJ
_RS06500
+0.3
2.1
class I SAM-dependent methyltransferase
compare
MMJJ
_RS06810
+0.3
2.5
L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha
compare
MMJJ
_RS07985
+0.3
1.5
hypothetical protein
compare
MMJJ
_RS04105
+0.3
2.5
prepilin peptidase
compare
MMJJ
_RS03515
+0.3
1.9
GPR1/FUN34/YaaH family transporter
compare
MMJJ
_RS08515
+0.3
1.4
secondary thiamine-phosphate synthase enzyme YjbQ
compare
MMJJ
_RS04270
+0.3
1.6
DUF364 domain-containing protein
compare
MMJJ
_RS07210
+0.3
2.2
AmmeMemoRadiSam system protein B
compare
MMJJ
_RS06685
+0.3
2.4
S-layer protein
compare
MMJJ
_RS04215
+0.3
2.3
DMT family transporter
compare
MMJJ
_RS03775
+0.3
0.8
50S ribosomal protein L15e
compare
MMJJ
_RS07015
+0.3
0.5
PFL family protein
compare
MMJJ
_RS06455
+0.3
2.1
YeeE/YedE family protein
compare
MMJJ
_RS06590
+0.3
2.2
sodium:alanine symporter family protein
compare
MMJJ
_RS08935
+0.3
0.4
TIGR00288 family NYN domain-containing protein
compare
MMJJ
_RS06205
+0.3
2.1
EamA family transporter
compare
MMJJ
_RS06800
+0.3
1.7
prefoldin subunit alpha
compare
MMJJ
_RS03375
+0.3
1.3
hypothetical protein
compare
MMJJ
_RS03360
+0.3
0.5
hypothetical protein
compare
MMJJ
_RS00145
+0.3
2.1
aquaporin family protein
compare
MMJJ
_RS06280
+0.3
2.0
hypothetical protein
compare
MMJJ
_RS08310
+0.3
1.5
ZIP family metal transporter
compare
MMJJ
_RS08410
+0.3
0.8
CBS domain-containing protein
compare
MMJJ
_RS06430
+0.3
1.6
haloacid dehalogenase
compare
MMJJ
_RS04635
+0.3
1.8
DUF367 family protein
compare
MMJJ
_RS05500
+0.3
2.3
hypothetical protein
compare
MMJJ
_RS07230
+0.3
1.7
coenzyme F420-reducing hydrogenase, FrhD protein
compare
MMJJ
_RS07495
+0.3
1.7
4Fe-4S binding protein
compare
MMJJ
_RS02285
+0.3
1.1
class III signal peptide-containing protein
compare
MMJJ
_RS06235
+0.3
2.1
IGHMBP2 family helicase
compare
MMJJ
_RS05390
+0.2
1.9
tRNA pseudouridine(13) synthase TruD
compare
MMJJ
_RS03605
+0.2
1.3
TIGR00297 family protein
compare
MMJJ
_RS06735
+0.2
1.7
cobalt ECF transporter T component CbiQ
compare
MMJJ
_RS02355
+0.2
1.7
DUF2798 domain-containing protein
compare
MMJJ
_RS03395
+0.2
0.5
hypothetical protein
compare
MMJJ
_RS01320
+0.2
1.8
indolepyruvate ferredoxin oxidoreductase subunit alpha
compare
MMJJ
_RS03720
+0.2
1.3
DsrE family protein
compare
MMJJ
_RS06580
+0.2
1.8
alanine dehydrogenase
compare
MMJJ
_RS05405
+0.2
1.8
DNA cytosine methyltransferase
compare
MMJJ
_RS09245
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS07905
+0.2
1.6
DUF89 domain-containing protein
compare
MMJJ
_RS06520
+0.2
1.7
TldD/PmbA family protein
compare
MMJJ
_RS06120
+0.2
1.6
DUF4013 domain-containing protein
compare
MMJJ
_RS06950
+0.2
1.6
4-demethylwyosine synthase TYW1
compare
MMJJ
_RS08165
+0.2
1.6
thermonuclease family protein
compare
MMJJ
_RS09360
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS07260
+0.2
1.6
PspC domain-containing protein
compare
MMJJ
_RS06055
+0.2
1.7
S-methyl-5-thioribose-1-phosphate isomerase
compare
MMJJ
_RS09065
+0.2
1.6
hypothetical protein
compare
MMJJ
_RS06960
+0.2
2.0
CAP domain-containing protein
compare
MMJJ
_RS06790
+0.2
1.9
nucleotidyltransferase family protein
compare
MMJJ
_RS06100
+0.2
1.6
rubrerythrin
compare
MMJJ
_RS06770
+0.2
1.9
hypothetical protein
compare
MMJJ
_RS05990
+0.2
1.4
phosphatase PAP2 family protein
compare
MMJJ
_RS06080
+0.2
1.6
serine/threonine protein kinase
compare
MMJJ
_RS04625
+0.2
1.6
SLC13 family permease
compare
MMJJ
_RS05980
+0.2
1.3
DUF5400 domain-containing protein
compare
MMJJ
_RS00730
+0.2
1.4
peptide-methionine (S)-S-oxide reductase MsrA
compare
MMJJ
_RS05975
+0.2
1.5
sulfurtransferase TusA family protein
compare
MMJJ
_RS07010
+0.2
1.3
preprotein translocase subunit Sec61beta
compare
MMJJ
_RS08045
+0.2
1.8
transporter substrate-binding domain-containing protein
compare
MMJJ
_RS04390
+0.2
1.7
rhomboid family intramembrane serine protease
compare
MMJJ
_RS08180
+0.2
1.3
ABC transporter ATP-binding protein
compare
MMJJ
_RS06535
+0.2
1.7
UPF0104 family protein
compare
MMJJ
_RS04225
+0.2
1.6
aldo/keto reductase
compare
MMJJ
_RS04780
+0.2
1.0
nucleic acid-binding protein
compare
MMJJ
_RS09205
+0.2
0.8
hypothetical protein
compare
MMJJ
_RS06585
+0.2
1.5
alanine racemase
compare
MMJJ
_RS07480
+0.2
1.7
FAD-dependent oxidoreductase
compare
MMJJ
_RS05780
+0.2
0.5
flagellin
compare
MMJJ
_RS00890
+0.2
1.4
coenzyme F420 hydrogenase subunit gamma
compare
MMJJ
_RS08285
+0.2
1.6
class I SAM-dependent methyltransferase
compare
MMJJ
_RS01745
+0.2
1.2
metal-dependent transcriptional regulator
compare
MMJJ
_RS07215
+0.2
1.7
TIGR00341 family protein
compare
MMJJ
_RS06765
+0.2
1.7
hypothetical protein
compare
MMJJ
_RS05350
+0.2
1.0
DUF192 domain-containing protein
compare
MMJJ
_RS07740
+0.2
1.4
DUF2334 domain-containing protein
compare
MMJJ
_RS05700
+0.2
1.4
metallophosphoesterase
compare
MMJJ
_RS06045
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS07760
+0.2
1.1
GNAT family N-acetyltransferase
compare
MMJJ
_RS07695
+0.2
1.7
glycoside hydrolase family 15 protein
compare
MMJJ
_RS03040
+0.2
1.3
lactate utilization protein
compare
MMJJ
_RS05355
+0.2
1.3
DUF2121 domain-containing protein
compare
MMJJ
_RS02270
+0.2
0.9
HEPN domain-containing protein
compare
MMJJ
_RS09120
+0.2
0.4
aspartate kinase
compare
MMJJ
_RS07385
+0.2
1.3
ribosomal protein S18-alanine N-acetyltransferase
compare
MMJJ
_RS01275
+0.2
0.9
4Fe-4S dicluster domain-containing protein
compare
MMJJ
_RS08160
+0.2
1.1
tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO
compare
MMJJ
_RS02265
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS00395
+0.2
1.0
DUF473 domain-containing protein
compare
MMJJ
_RS08050
+0.2
1.5
ABC transporter substrate-binding protein
compare
MMJJ
_RS05660
+0.2
1.2
sulfopyruvate decarboxylase subunit beta
compare
MMJJ
_RS04545
+0.2
0.5
PadR family transcriptional regulator
compare
MMJJ
_RS06550
+0.2
1.3
ABC transporter permease
compare
MMJJ
_RS02665
+0.2
0.4
methyl-accepting chemotaxis protein
compare
MMJJ
_RS08580
+0.2
0.8
YkgJ family cysteine cluster protein
compare
MMJJ
_RS05005
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS07200
+0.2
1.4
MFS transporter
compare
MMJJ
_RS05585
+0.2
1.2
hypothetical protein
compare
MMJJ
_RS06210
+0.2
1.0
hypothetical protein
compare
MMJJ
_RS06710
+0.2
1.4
TldD/PmbA family protein
compare
MMJJ
_RS08670
+0.2
1.2
phosphate signaling complex protein PhoU
compare
MMJJ
_RS06400
+0.2
0.6
FumA C-terminus/TtdB family hydratase beta subunit
compare
MMJJ
_RS05150
+0.2
1.1
5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH
compare
MMJJ
_RS05510
+0.2
1.2
archaellum operon transcriptional activator EarA family protein
compare
MMJJ
_RS01955
+0.2
1.4
hypothetical protein
compare
MMJJ
_RS07820
+0.2
1.3
bifunctional hexulose-6-phosphate synthase/ribonuclease regulator
compare
MMJJ
_RS06555
+0.2
1.3
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS05965
+0.2
1.3
MFS transporter
compare
MMJJ
_RS01825
+0.2
0.9
DNA repair and recombination protein RadB
compare
MMJJ
_RS01405
+0.2
0.1
proline--tRNA ligase
compare
MMJJ
_RS09295
+0.2
0.2
hypothetical protein
compare
MMJJ
_RS06740
+0.2
0.8
energy-coupling factor ABC transporter substrate-binding protein
compare
MMJJ
_RS05505
+0.2
1.5
hypothetical protein
compare
MMJJ
_RS08375
+0.2
1.1
ferritin
compare
MMJJ
_RS07835
+0.2
1.0
UPF0489 family protein
compare
MMJJ
_RS00525
+0.2
0.4
Dabb family protein
compare
MMJJ
_RS00155
+0.2
1.3
DUF2193 domain-containing protein
compare
MMJJ
_RS07255
+0.2
1.3
hypothetical protein
compare
MMJJ
_RS03510
+0.2
1.1
acyl-CoA dehydratase activase
compare
MMJJ
_RS01035
+0.2
0.7
hypothetical protein
compare
MMJJ
_RS04965
+0.2
0.5
RNA polymerase Rpb4 family protein
compare
MMJJ
_RS06465
+0.1
0.8
hypothetical protein
compare
MMJJ
_RS02230
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS07815
+0.1
1.1
2-oxoacid:acceptor oxidoreductase family protein
compare
MMJJ
_RS08240
+0.1
1.0
L-fuculose phosphate aldolase
compare
MMJJ
_RS02170
+0.1
0.9
2-thiouracil desulfurase family protein
compare
MMJJ
_RS08315
+0.1
1.0
DNA protection protein DPS
compare
MMJJ
_RS02165
+0.1
1.0
ABC transporter substrate-binding protein
compare
MMJJ
_RS03955
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS07890
+0.1
0.9
pyrimidine dimer DNA glycosylase/endonuclease V
compare
MMJJ
_RS02065
+0.1
1.2
S-layer protein
compare
MMJJ
_RS05900
+0.1
1.1
ATP-binding cassette domain-containing protein
compare
MMJJ
_RS03595
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS09005
+0.1
1.2
N-6 DNA methylase
compare
MMJJ
_RS05580
+0.1
0.9
methanogenesis marker 9 domain-containing protein
compare
MMJJ
_RS05835
+0.1
1.1
bis-aminopropyl spermidine synthase family protein
compare
MMJJ
_RS03545
+0.1
1.1
flagella protein F
compare
MMJJ
_RS00405
+0.1
0.8
flavodoxin family protein
compare
MMJJ
_RS06275
+0.1
0.7
hypothetical protein
compare
MMJJ
_RS07280
+0.1
0.8
phosphomannomutase/phosphoglucomutase
compare
MMJJ
_RS01430
+0.1
0.9
hypothetical protein
compare
MMJJ
_RS08210
+0.1
1.3
hypothetical protein
compare
MMJJ
_RS05335
+0.1
1.0
heavy metal-binding domain-containing protein
compare
MMJJ
_RS05410
+0.1
0.8
RecB-family nuclease
compare
MMJJ
_RS02895
+0.1
1.0
ATP-binding protein
compare
MMJJ
_RS05690
+0.1
1.2
hypothetical protein
compare
MMJJ
_RS01910
+0.1
1.1
class III signal peptide-containing protein
compare
MMJJ
_RS00680
+0.1
1.1
nitrogenase iron-molybdenum cofactor biosynthesis protein NifE
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis JJ in nutrient t1 experiments