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Experiment set3IT034 for
Methanococcus maripaludis S2
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H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
200 most detrimental genes:
gene
name
fitness
t score
description
MMP
_RS08860
+3.1
21.4
MinD/ParA family protein
compare
MMP
_RS08855
+3.1
22.6
hypothetical protein
compare
MMP
_RS00240
+2.9
25.1
type II secretion system F family protein
compare
MMP
_RS08675
+2.9
13.6
class III signal peptide-containing protein
compare
MMP
_RS00245
+2.9
25.7
type II secretion system F family protein
compare
MMP
_RS01280
+2.8
24.0
DUF515 domain-containing protein
compare
MMP
_RS01285
+2.8
24.3
hypothetical protein
compare
MMP
_RS01270
+2.8
23.9
prepilin peptidase
compare
MMP
_RS01290
+2.8
23.5
class III signal peptide-containing protein
compare
MMP
_RS00250
+2.8
22.9
Flp pilus assembly complex ATPase component TadA
compare
MMP
_RS01305
+2.8
25.7
hypothetical protein
compare
MMP
_RS01310
+2.7
25.3
hypothetical protein
compare
MMP
_RS01295
+2.7
21.9
hypothetical protein
compare
MMP
_RS09055
+2.6
4.2
CAP domain-containing protein
compare
MMP
_RS01315
+2.6
23.8
DUF2341 domain-containing protein
compare
MMP
_RS01195
+2.6
4.9
metalloregulator ArsR/SmtB family transcription factor
compare
MMP
_RS01275
+2.6
14.8
class III signal peptide-containing protein
compare
MMP
_RS01085
+2.5
6.9
hypothetical protein
compare
MMP
_RS05635
+2.5
17.2
NAD-dependent epimerase/dehydratase family protein
compare
MMP
_RS08940
+2.4
4.7
MetS family NSS transporter small subunit
compare
MMP
_RS07330
+2.4
22.3
membrane protein
compare
MMP
_RS03125
+2.2
14.9
cell division ATPase MinD
compare
MMP
_RS06035
+2.1
9.8
glycosyltransferase family 2 protein
compare
MMP
_RS08615
+2.0
2.3
flagellar protein G
compare
MMP
_RS09045
+1.7
10.3
class III signal peptide-containing protein
compare
MMP
_RS01075
+1.5
2.5
hypothetical protein
compare
MMP
_RS06600
+1.4
1.2
class III signal peptide-containing protein
compare
MMP
_RS05865
+1.4
1.4
hypothetical protein
compare
MMP
_RS00535
+1.4
2.3
TIGR00375 family protein
compare
MMP
_RS05580
+1.4
11.2
glycosyltransferase
compare
MMP
_RS08580
+1.4
4.0
flagellin
compare
MMP
_RS00140
+1.4
7.8
DUF192 domain-containing protein
compare
MMP
_RS00570
+1.4
1.2
protein-L-isoaspartate O-methyltransferase
compare
MMP
_RS03080
+1.4
1.9
universal stress protein
compare
MMP
_RS04125
+1.3
2.7
TetR/AcrR family transcriptional regulator
compare
MMP
_RS01790
+1.3
2.7
restriction endonuclease
compare
MMP
_RS08750
+1.3
1.5
hypothetical protein
compare
MMP
_RS05985
+1.3
1.9
hypothetical protein
compare
MMP
_RS07900
+1.2
2.1
YeeE/YedE family protein
compare
MMP
_RS08845
+1.2
4.4
archaellum operon transcriptional activator EarA family protein
compare
MMP
_RS03550
+1.2
1.2
DUF2124 domain-containing protein
compare
MMP
_RS04410
+1.2
2.4
hypothetical protein
compare
MMP
_RS08830
+1.2
3.7
GTP-binding protein
compare
MMP
_RS02545
+1.2
2.0
hypothetical protein
compare
MMP
_RS06720
+1.1
4.0
response regulator transcription factor
compare
MMP
_RS05965
+1.0
1.7
carboxymuconolactone decarboxylase family protein
compare
MMP
_RS08400
+1.0
0.9
MJ0307 family thioredoxin
compare
MMP
_RS01255
+1.0
1.8
amino acid ABC transporter ATP-binding protein
compare
MMP
_RS03625
+0.9
2.5
cell wall-binding protein
compare
MMP
_RS06935
+0.9
2.5
protease complex subunit PrcB family protein
compare
MMP
_RS06715
+0.9
5.8
cache domain-containing protein
compare
MMP
_RS08850
+0.9
6.0
hypothetical protein
compare
MMP
_RS05910
+0.9
1.7
cell division ATPase MinD
compare
MMP
_RS07345
+0.9
2.2
glycine synthesis protein GlyXL
(from data)
compare
MMP
_RS08410
+0.8
1.9
DUF169 domain-containing protein
compare
MMP
_RS01125
+0.8
2.4
thioredoxin family protein
compare
MMP
_RS02530
+0.8
1.9
type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family
compare
MMP
_RS06760
+0.8
1.0
hypothetical protein
compare
MMP
_RS08610
+0.8
3.6
flagellar protein F
compare
MMP
_RS05785
+0.8
4.4
ATP-binding protein
compare
MMP
_RS08395
+0.8
1.8
DsrE family protein
compare
MMP
_RS01870
+0.7
1.8
acyl-CoA dehydratase activase
compare
MMP
_RS00345
+0.7
4.1
DUF2666 domain-containing protein
compare
MMP
_RS07775
+0.7
1.5
alanine dehydrogenase
compare
MMP
_RS01655
+0.7
1.0
DsrE family protein
compare
MMP
_RS00690
+0.7
2.0
MBL fold metallo-hydrolase
compare
MMP
_RS00090
+0.7
1.4
CoA-binding protein
compare
MMP
_RS03120
+0.7
1.5
hypothetical protein
compare
MMP
_RS07550
+0.7
2.5
L-serine ammonia-lyase iron-sulfur-dependent subunit alpha
compare
MMP
_RS08080
+0.7
2.3
YchF/TatD family DNA exonuclease
compare
MMP
_RS01300
+0.7
5.2
hypothetical protein
compare
MMP
_RS08770
+0.7
2.5
hypothetical protein
compare
MMP
_RS07765
+0.7
2.8
sodium:alanine symporter family protein
compare
MMP
_RS07800
+0.7
2.0
ATP-binding cassette domain-containing protein
compare
MMP
_RS08285
+0.7
2.4
serine/threonine protein kinase
compare
MMP
_RS04255
+0.6
1.6
coenzyme F420 hydrogenase subunit beta
compare
MMP
_RS01490
+0.6
1.4
tetratricopeptide repeat protein
compare
MMP
_RS08385
+0.6
1.3
ABC transporter substrate-binding protein
compare
MMP
_RS02255
+0.6
1.7
hypothetical protein
compare
MMP
_RS01135
+0.6
2.6
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS06115
+0.6
2.6
class II aldolase/adducin family protein
compare
MMP
_RS08290
+0.6
2.0
DNA-binding protein Alba
compare
MMP
_RS06100
+0.6
2.1
GNAT family N-acetyltransferase
compare
MMP
_RS04990
+0.6
1.9
aquaporin family protein
compare
MMP
_RS02260
+0.6
2.0
lactate utilization protein
compare
MMP
_RS03535
+0.6
0.8
helix-turn-helix transcriptional regulator
compare
MMP
_RS00710
+0.6
1.0
hypothetical protein
compare
MMP
_RS01635
+0.6
1.7
thiamine pyrophosphate-dependent enzyme
compare
MMP
_RS06140
+0.5
1.1
radical SAM protein
compare
MMP
_RS08490
+0.5
3.5
hypothetical protein
compare
MMP
_RS07855
+0.5
2.5
class I SAM-dependent methyltransferase
compare
MMP
_RS06015
+0.5
1.1
flavodoxin family protein
compare
MMP
_RS06175
+0.5
2.3
winged helix-turn-helix transcriptional regulator
compare
MMP
_RS04290
+0.5
1.4
4Fe-4S binding protein
compare
MMP
_RS08280
+0.5
1.5
proteasome assembly chaperone family protein
compare
MMP
_RS02610
+0.5
1.0
DUF2162 domain-containing protein
compare
MMP
_RS00965
+0.5
0.6
hypothetical protein
compare
MMP
_RS06050
+0.5
1.6
ZIP family metal transporter
compare
MMP
_RS08665
+0.5
3.5
hypothetical protein
compare
MMP
_RS02540
+0.5
1.2
DUF2283 domain-containing protein
compare
MMP
_RS02605
+0.5
0.4
MotA/TolQ/ExbB proton channel family protein
compare
MMP
_RS00480
+0.5
1.4
hypothetical protein
compare
MMP
_RS05685
+0.5
1.6
winged helix-turn-helix domain-containing protein
compare
MMP
_RS01140
+0.5
0.8
molybdate ABC transporter permease subunit
compare
MMP
_RS06990
+0.5
1.7
CatB-related O-acetyltransferase
compare
MMP
_RS03530
+0.5
2.1
tetratricopeptide repeat protein
compare
MMP
_RS07570
+0.5
1.9
nucleotidyltransferase family protein
compare
MMP
_RS02425
+0.5
1.3
hypothetical protein
compare
MMP
_RS02295
+0.5
2.8
TatD family hydrolase
compare
MMP
_RS06445
+0.5
1.1
DUF89 domain-containing protein
compare
MMP
_RS08380
+0.5
2.7
phosphatase PAP2 family protein
compare
MMP
_RS01630
+0.5
3.3
N-glycosylase/DNA lyase
compare
MMP
_RS00905
+0.5
1.1
phenylacetate--CoA ligase
compare
MMP
_RS08405
+0.5
2.4
MFS transporter
compare
MMP
_RS03060
+0.5
1.3
MBL fold metallo-hydrolase
compare
MMP
_RS07925
+0.5
2.2
haloacid dehalogenase
compare
MMP
_RS07640
+0.5
1.4
mechanosensitive ion channel family protein
compare
MMP
_RS06460
+0.5
0.9
pyrimidine dimer DNA glycosylase/endonuclease V
compare
MMP
_RS01660
+0.5
0.7
hypothetical protein
compare
MMP
_RS08250
+0.5
1.8
pyruvate kinase
compare
MMP
_RS01605
+0.5
1.4
hypothetical protein
compare
MMP
_RS03305
+0.5
2.5
winged helix-turn-helix domain-containing protein
compare
MMP
_RS02455
+0.5
1.6
hypothetical protein
compare
MMP
_RS02735
+0.5
1.6
MBL fold metallo-hydrolase
compare
MMP
_RS08205
+0.5
2.6
stage II sporulation protein M
compare
MMP
_RS07805
+0.5
1.7
ABC transporter permease
compare
MMP
_RS01185
+0.5
1.3
hypothetical protein
compare
MMP
_RS06090
+0.5
1.1
iron ABC transporter permease
compare
MMP
_RS08485
+0.5
2.4
tungstate ABC transporter substrate-binding protein WtpA
compare
MMP
_RS04995
+0.4
1.6
hypothetical protein
compare
MMP
_RS06385
+0.4
0.9
hypothetical protein
compare
MMP
_RS02630
+0.4
1.6
rubredoxin
compare
MMP
_RS03565
+0.4
1.5
uracil phosphoribosyltransferase
compare
MMP
_RS02625
+0.4
1.3
ribonuclease H-like domain-containing protein
compare
MMP
_RS06250
+0.4
1.5
hypothetical protein
compare
MMP
_RS00400
+0.4
2.6
tributyrin esterase
compare
MMP
_RS04390
+0.4
1.7
hypothetical protein
compare
MMP
_RS02020
+0.4
0.8
gamma-glutamylcyclotransferase
compare
MMP
_RS06975
+0.4
0.8
sulfide-dependent adenosine diphosphate thiazole synthase
compare
MMP
_RS07770
+0.4
1.3
alanine racemase
compare
MMP
_RS04610
+0.4
0.8
Dabb family protein
compare
MMP
_RS08415
+0.4
1.2
radical SAM protein
compare
MMP
_RS06905
+0.4
1.0
hypothetical protein
compare
MMP
_RS02635
+0.4
1.5
class I SAM-dependent methyltransferase
compare
MMP
_RS01770
+0.4
1.9
ATP-dependent endonuclease
compare
MMP
_RS08475
+0.4
1.6
ABC transporter permease
compare
MMP
_RS01320
+0.4
1.0
hypothetical protein
compare
MMP
_RS02770
+0.4
0.8
hypothetical protein
compare
MMP
_RS05140
+0.4
1.3
helix-turn-helix transcriptional regulator
compare
MMP
_RS07905
+0.4
1.1
DUF3343 domain-containing protein
compare
MMP
_RS00540
+0.4
1.4
PLP-dependent aminotransferase family protein
compare
MMP
_RS01570
+0.4
0.6
hypothetical protein
compare
MMP
_RS01765
+0.4
1.0
HIT family protein
compare
MMP
_RS02215
+0.4
1.8
ECF transporter S component
compare
MMP
_RS07590
+0.4
2.3
hypothetical protein
compare
MMP
_RS07910
+0.4
1.3
NAD(P)H-hydrate dehydratase
compare
MMP
_RS01215
+0.4
1.5
sodium/proline symporter PutP
compare
MMP
_RS08245
+0.4
1.5
DUF4013 domain-containing protein
compare
MMP
_RS03670
+0.4
1.3
DUF1890 domain-containing protein
compare
MMP
_RS04600
+0.4
2.8
tetratricopeptide repeat protein
compare
MMP
_RS08640
+0.4
2.5
TIM barrel protein
compare
MMP
_RS03525
+0.4
1.6
ketopantoate reductase family protein
compare
MMP
_RS07880
+0.4
1.3
AAA family ATPase
compare
MMP
_RS06665
+0.4
1.4
glycogen/starch synthase
compare
MMP
_RS08835
+0.4
2.0
H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein
compare
MMP
_RS08980
+0.4
0.9
hypothetical protein
compare
MMP
_RS01070
+0.4
2.0
hypothetical protein
compare
MMP
_RS06490
+0.4
1.5
hypothetical protein
compare
MMP
_RS08455
+0.4
1.6
multiprotein bridging factor aMBF1
compare
MMP
_RS08390
+0.4
1.3
DUF5400 domain-containing protein
compare
MMP
_RS02320
+0.4
1.6
glutamate-cysteine ligase family protein
compare
MMP
_RS08230
+0.4
0.9
50S ribosomal protein L11 methyltransferase
compare
MMP
_RS08930
+0.4
0.7
hypothetical protein
compare
MMP
_RS09060
+0.4
2.4
hypothetical protein
compare
MMP
_RS04055
+0.4
0.6
hypothetical protein
compare
MMP
_RS06710
+0.4
1.6
selenium-binding protein
compare
MMP
_RS01675
+0.3
0.6
hypothetical protein
compare
MMP
_RS02830
+0.3
1.2
DUF432 domain-containing protein
compare
MMP
_RS02850
+0.3
1.5
hypothetical protein
compare
MMP
_RS06970
+0.3
1.2
ribosomal protein S18-alanine N-acetyltransferase
compare
MMP
_RS00555
+0.3
0.7
MFS transporter
compare
MMP
_RS07985
+0.3
2.1
nitroreductase family protein
compare
MMP
_RS01110
+0.3
0.4
FmdE family protein
compare
MMP
_RS08775
+0.3
1.3
methanogenesis marker 9 domain-containing protein
compare
MMP
_RS01690
+0.3
1.3
indolepyruvate ferredoxin oxidoreductase subunit alpha
compare
MMP
_RS08265
+0.3
1.5
rubrerythrin
compare
MMP
_RS05780
+0.3
1.6
multidrug resistance efflux transporter family protein
compare
MMP
_RS06965
+0.3
2.0
TIGR04014 family B12-binding domain/radical SAM domain-containing protein
compare
MMP
_RS08480
+0.3
2.4
molybdate ABC transporter substrate-binding protein
compare
MMP
_RS07085
+0.3
1.4
ribonuclease HII
compare
MMP
_RS04975
+0.3
1.8
hypothetical protein
compare
MMP
_RS05575
+0.3
1.7
hypothetical protein
compare
MMP
_RS08670
+0.3
1.6
metallophosphoesterase
compare
MMP
_RS06300
+0.3
1.2
ABC transporter substrate-binding protein
compare
MMP
_RS02710
+0.3
1.5
formylmethanofuran dehydrogenase subunit B
compare
MMP
_RS01495
+0.3
1.2
TraB/GumN family protein
compare
MMP
_RS08620
+0.3
1.6
flagellar accessory protein FlaH
compare
MMP
_RS03900
+0.3
1.1
minichromosome maintenance protein MCM
compare
MMP
_RS02660
+0.3
2.2
hypothetical protein
compare
MMP
_RS02670
+0.3
1.5
ATP-binding cassette domain-containing protein
compare
Specific Phenotypes
None in this experiment
For Methanococcus maripaludis S2 in rich t2 experiments