Experiment set3IT033 for Pseudomonas fluorescens FW300-N1B4

Compare to:

LB with 1-ethyl-3-methylimidazolium lysine 5 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf1N1B4_2380 +2.2 18.1 Cytochrome B561 compare
Pf1N1B4_2381 +2.1 17.1 Protein yceI precursor compare
Pf1N1B4_5924 +2.1 16.5 hypothetical protein compare
Pf1N1B4_5926 +2.1 16.1 hypothetical protein compare
Pf1N1B4_5925 +1.9 14.8 hypothetical protein compare
Pf1N1B4_5927 +1.4 12.9 Capsular polysaccharide biosynthesis protein compare
Pf1N1B4_3006 +1.4 7.9 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf1N1B4_3005 +1.4 10.4 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf1N1B4_733 +1.3 2.7 Methionyl-tRNA synthetase (EC 6.1.1.10) compare
Pf1N1B4_751 +1.3 2.9 hypothetical protein compare
Pf1N1B4_623 +1.3 2.4 Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10) compare
Pf1N1B4_4473 +1.3 2.8 Homoserine/homoserine lactone efflux protein compare
Pf1N1B4_2145 +1.3 2.7 Translation initiation inhibitor compare
Pf1N1B4_731 +1.3 2.8 Scaffold protein for [4Fe-4S] cluster assembly ApbC, MRP-like compare
Pf1N1B4_2724 +1.2 7.4 Transcriptional regulator, AsnC family compare
Pf1N1B4_1668 +1.2 6.7 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf1N1B4_678 +1.1 6.6 Pyruvate kinase (EC 2.7.1.40) compare
Pf1N1B4_2117 +1.1 9.4 Ribonuclease PH (EC 2.7.7.56) compare
Pf1N1B4_2154 +1.1 3.3 hypothetical protein compare
Pf1N1B4_1882 +1.1 10.5 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf1N1B4_240 +1.1 2.9 Transcriptional regulators compare
Pf1N1B4_2637 +1.1 5.3 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) compare
Pf1N1B4_262 +1.1 9.3 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf1N1B4_2701 +1.1 9.9 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf1N1B4_647 +1.1 7.0 hypothetical protein compare
Pf1N1B4_1468 +1.1 8.9 InaA protein compare
Pf1N1B4_901 +1.1 3.0 hypothetical protein compare
Pf1N1B4_3091 +1.0 4.7 Membrane-associated zinc metalloprotease compare
Pf1N1B4_4858 +1.0 4.9 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf1N1B4_2545 +1.0 8.2 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf1N1B4_2382 +1.0 7.0 ATP-dependent RNA helicase RhlE compare
Pf1N1B4_1838 +1.0 1.9 FIG00955509: hypothetical protein compare
Pf1N1B4_292 +1.0 9.6 ErfK/YbiS/YcfS/YnhG family protein compare
Pf1N1B4_710 +1.0 3.3 Transporter, LysE family compare
Pf1N1B4_291 +1.0 6.2 Major outer membrane lipoprotein I compare
Pf1N1B4_969 +1.0 9.6 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf1N1B4_2870 +1.0 2.1 Regulator of competence-specific genes compare
Pf1N1B4_232 +1.0 2.2 DNA-binding response regulator, LuxR family compare
Pf1N1B4_1246 +1.0 2.0 FIG00954047: hypothetical protein compare
Pf1N1B4_65 +1.0 5.8 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf1N1B4_2144 +1.0 6.3 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf1N1B4_2401 +1.0 1.7 Lipoprotein, putative compare
Pf1N1B4_959 +1.0 8.0 Stringent starvation protein A compare
Pf1N1B4_2878 +1.0 6.4 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf1N1B4_1035 +1.0 3.8 Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C) compare
Pf1N1B4_1475 +0.9 5.0 FIG00962376: hypothetical protein compare
Pf1N1B4_5383 +0.9 6.9 hypothetical protein compare
Pf1N1B4_1889 +0.9 8.2 Fused spore maturation proteins A and B compare
Pf1N1B4_2850 +0.9 6.3 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf1N1B4_4553 +0.9 2.8 FIG00959354: hypothetical protein compare
Pf1N1B4_1830 +0.9 6.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf1N1B4_2549 +0.9 5.6 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf1N1B4_4403 +0.9 2.2 NADH dehydrogenase (EC 1.6.99.3) compare
Pf1N1B4_2168 +0.9 5.5 FIG004064: hypothetical protein compare
Pf1N1B4_5382 +0.9 3.1 hypothetical protein compare
Pf1N1B4_3241 +0.9 2.0 hypothetical protein compare
Pf1N1B4_1069 +0.9 4.3 YrbA protein compare
Pf1N1B4_2879 +0.9 5.5 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf1N1B4_970 +0.9 7.2 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf1N1B4_5916 +0.9 2.7 Similar to non-heme chloroperoxidase, sll5080 homolog compare
Pf1N1B4_3829 +0.9 6.9 Magnesium and cobalt transport protein CorA compare
Pf1N1B4_3507 +0.9 7.0 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf1N1B4_740 +0.9 1.9 Leucyl-tRNA synthetase compare
Pf1N1B4_1181 +0.9 2.8 UPF0125 protein yfjF compare
Pf1N1B4_5745 +0.9 1.3 FIG139928: Putative protease compare
Pf1N1B4_1704 +0.9 3.1 hypothetical protein compare
Pf1N1B4_290 +0.9 8.0 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_572 +0.9 7.0 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_3152 +0.9 4.6 LysR family transcriptional regulator PA3398 compare
Pf1N1B4_1545 +0.9 6.4 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf1N1B4_3828 +0.8 5.3 Ribonuclease E inhibitor RraA compare
Pf1N1B4_2547 +0.8 2.8 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf1N1B4_1135 +0.8 2.2 FIG00960055: hypothetical protein compare
Pf1N1B4_5574 +0.8 2.4 hypothetical protein compare
Pf1N1B4_1469 +0.8 7.5 Heptose kinase WapQ, eukaryotic type compare
Pf1N1B4_4975 +0.8 2.2 hypothetical protein compare
Pf1N1B4_971 +0.8 7.1 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf1N1B4_590 +0.8 6.3 Phosphogluconate repressor HexR, RpiR family compare
Pf1N1B4_1570 +0.8 3.3 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf1N1B4_5275 +0.8 1.6 hypothetical protein compare
Pf1N1B4_4208 +0.8 1.8 organophosphate pesticide hydrolase compare
Pf1N1B4_5115 +0.8 2.2 sucrose ABC transporter, ATPase component (from data) compare
Pf1N1B4_5930 +0.8 4.4 Exopolysaccharide production protein ExoZ compare
Pf1N1B4_3982 +0.8 3.4 FIG00953347: hypothetical protein compare
Pf1N1B4_3229 +0.8 4.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf1N1B4_885 +0.8 2.5 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_2853 +0.8 1.6 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
Pf1N1B4_817 +0.8 2.3 FIG00955104: hypothetical protein compare
Pf1N1B4_3113 +0.8 5.9 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf1N1B4_4358 +0.8 1.9 Acetyltransferase, GNAT family compare
Pf1N1B4_2526 +0.8 6.7 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf1N1B4_2178 +0.8 1.8 Regulator of nucleoside diphosphate kinase compare
Pf1N1B4_3273 +0.8 1.8 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf1N1B4_723 +0.8 5.8 two-component system sensor protein compare
Pf1N1B4_4826 +0.8 1.6 glutamine synthetase family protein compare
Pf1N1B4_1194 +0.8 1.3 hypothetical protein compare
Pf1N1B4_1695 +0.8 1.6 Cystine ABC transporter, ATP-binding protein compare
Pf1N1B4_3824 +0.7 5.4 FIG137360: hypothetical protein compare
Pf1N1B4_4594 +0.7 1.4 Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) compare
Pf1N1B4_1554 +0.7 4.5 predicted FeS cluster maintenance protein (from data) compare
Pf1N1B4_5864 +0.7 1.7 Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF compare
Pf1N1B4_337 +0.7 2.9 O-antigen export system permease protein RfbD compare
Pf1N1B4_4132 +0.7 1.5 Mobile element protein compare
Pf1N1B4_2071 +0.7 1.6 Protein phosphatase ImpM compare
Pf1N1B4_656 +0.7 4.9 Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1) compare
Pf1N1B4_5224 +0.7 1.4 tRNA-Arg-CCT compare
Pf1N1B4_284 +0.7 5.8 Universal stress protein family compare
Pf1N1B4_5813 +0.7 1.3 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) compare
Pf1N1B4_972 +0.7 6.7 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf1N1B4_5507 +0.7 1.6 hypothetical protein compare
Pf1N1B4_909 +0.7 1.8 Ketosteroid isomerase-related protein compare
Pf1N1B4_293 +0.7 4.1 hypothetical protein compare
Pf1N1B4_4700 +0.7 1.9 FIG00956898: hypothetical protein compare
Pf1N1B4_407 +0.7 1.4 ADP-ribosylglycohydrolase YegU (EC 3.2.-.-) compare
Pf1N1B4_1365 +0.7 1.7 Quaternary ammonium compound-resistance protein SugE compare
Pf1N1B4_2471 +0.7 2.7 putative iron-regulated membrane protein compare
Pf1N1B4_2163 +0.7 1.4 hypothetical protein compare
Pf1N1B4_3153 +0.7 1.5 hypothetical protein compare
Pf1N1B4_2694 +0.7 5.4 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf1N1B4_5801 +0.7 1.9 hypothetical protein compare
Pf1N1B4_2483 +0.7 2.1 DNA-binding domain of ModE / Molybdate-binding domain of ModE compare
Pf1N1B4_4866 +0.7 1.7 hypothetical protein compare
Pf1N1B4_1868 +0.7 4.1 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf1N1B4_973 +0.7 6.1 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf1N1B4_3965 +0.7 1.7 FIG033889: YebC paralog in Betaproteobacteria compare
Pf1N1B4_4029 +0.7 2.0 NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3) compare
Pf1N1B4_483 +0.7 1.8 Metal-dependent hydrolase related to alanyl-tRNA synthetase compare
Pf1N1B4_3830 +0.7 2.7 CrfX protein compare
Pf1N1B4_1334 +0.7 1.6 FIG00955840: hypothetical protein compare
Pf1N1B4_4295 +0.7 2.0 FIG00954434: hypothetical protein compare
Pf1N1B4_4172 +0.7 1.0 hypothetical protein compare
Pf1N1B4_749 +0.7 1.2 hypothetical protein compare
Pf1N1B4_5604 +0.7 1.9 Permeases of the major facilitator superfamily compare
Pf1N1B4_4220 +0.7 1.3 Maebl compare
Pf1N1B4_4893 +0.7 2.2 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) compare
Pf1N1B4_882 +0.7 1.9 carbonic anhydrase, family 3 compare
Pf1N1B4_4195 +0.7 2.3 Transcriptional regulator, TetR family compare
Pf1N1B4_648 +0.7 6.2 Outer membrane porin, OprD family compare
Pf1N1B4_3591 +0.6 3.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41) compare
Pf1N1B4_4831 +0.6 1.6 Outer membrane protein assembly factor YaeT precursor compare
Pf1N1B4_2240 +0.6 2.4 Transcriptional regulator, AsnC family compare
Pf1N1B4_2776 +0.6 2.1 Transcriptional regulator, GntR family compare
Pf1N1B4_251 +0.6 1.4 DNA-binding response regulator, LuxR family compare
Pf1N1B4_643 +0.6 3.6 FIG00955846: hypothetical protein compare
Pf1N1B4_4810 +0.6 2.0 Polymyxin resistance protein PmrM compare
Pf1N1B4_2734 +0.6 1.3 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf1N1B4_2947 +0.6 2.8 Restriction endonuclease compare
Pf1N1B4_287 +0.6 3.8 Possible periplasmic thiredoxin compare
Pf1N1B4_5718 +0.6 1.6 Transcriptional regulator, Cro/CI family compare
Pf1N1B4_1636 +0.6 1.2 FIG00954739: hypothetical protein compare
Pf1N1B4_3834 +0.6 4.3 Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf1N1B4_1122 +0.6 5.1 Outer membrane porin, OprD family compare
Pf1N1B4_1472 +0.6 1.4 Lipopolysaccharide heptosyltransferase I (EC 2.4.1.-) compare
Pf1N1B4_1632 +0.6 2.3 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf1N1B4_367 +0.6 2.4 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf1N1B4_447 +0.6 5.7 FIG004453: protein YceG like compare
Pf1N1B4_5509 +0.6 1.6 hypothetical protein compare
Pf1N1B4_2370 +0.6 2.3 Malonate utilization transcriptional regulator compare
Pf1N1B4_3630 +0.6 2.0 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) compare
Pf1N1B4_4870 +0.6 1.2 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf1N1B4_932 +0.6 1.0 FIG00963370: hypothetical protein compare
Pf1N1B4_5710 +0.6 2.1 Transcriptional regulator, TetR family compare
Pf1N1B4_2736 +0.6 1.7 ATP-dependent RNA helicase PA3950 compare
Pf1N1B4_4801 +0.6 1.8 Polysaccharide deacetylase compare
Pf1N1B4_2169 +0.6 1.6 FIG00953808: hypothetical protein compare
Pf1N1B4_47 +0.6 2.3 Copper resistance protein D compare
Pf1N1B4_201 +0.6 1.1 ABC transporter in pyoverdin gene cluster, ATP-binding component compare
Pf1N1B4_5708 +0.6 2.5 Transcriptional regulator, TetR family compare
Pf1N1B4_304 +0.6 1.3 Deoxyribonuclease TatD compare
Pf1N1B4_5949 +0.6 1.3 Phosphomannomutase (EC 5.4.2.8) compare
Pf1N1B4_5292 +0.6 1.6 hypothetical protein compare
Pf1N1B4_4041 +0.6 1.7 NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3) compare
Pf1N1B4_673 +0.6 1.8 MoxR-like ATPases compare
Pf1N1B4_1441 +0.6 4.5 Putative esterase, FIGfam005057 compare
Pf1N1B4_1667 +0.6 1.8 Histone acetyltransferase HPA2 and related acetyltransferases compare
Pf1N1B4_1095 +0.6 4.6 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf1N1B4_1313 +0.6 3.4 tRNA dihydrouridine synthase B (EC 1.-.-.-) compare
Pf1N1B4_2008 +0.6 3.9 Phosphogluconate repressor HexR, RpiR family compare
Pf1N1B4_2353 +0.6 2.2 twitching motility protein PilG compare
Pf1N1B4_5135 +0.6 1.8 Transcriptional regulator, TetR family compare
Pf1N1B4_1831 +0.6 4.6 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf1N1B4_4040 +0.6 1.9 NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3) compare
Pf1N1B4_1669 +0.6 3.4 ADP compounds hydrolase NudE (EC 3.6.1.-) compare
Pf1N1B4_1527 +0.6 2.0 Queuosine Biosynthesis QueC ATPase compare
Pf1N1B4_4863 +0.6 2.3 Outer membrane protein OprN compare
Pf1N1B4_4402 +0.6 1.1 SoxH protein, homolog compare
Pf1N1B4_4932 +0.6 1.7 Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83) compare
Pf1N1B4_4470 +0.6 1.7 Cobalt-zinc-cadmium resistance protein CzcD compare
Pf1N1B4_2715 +0.6 3.1 hypothetical protein compare
Pf1N1B4_3590 +0.6 3.2 hypothetical protein compare
Pf1N1B4_1512 +0.6 3.2 FIG021862: membrane protein, exporter compare
Pf1N1B4_1847 +0.6 2.4 hypothetical protein compare
Pf1N1B4_1333 +0.6 2.4 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf1N1B4_4990 +0.6 1.5 FIG018171: hypothetical protein of Cupin superfamily compare
Pf1N1B4_573 +0.6 3.9 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf1N1B4_152 +0.6 1.4 ABC transporter, permease protein compare
Pf1N1B4_1770 +0.6 1.9 Uncharacterized protein, possibly involved in aromatic compounds catabolism compare
Pf1N1B4_5976 +0.6 1.4 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22) compare
Pf1N1B4_2096 +0.6 1.3 Protein serine/threonine phosphatase PrpC, regulation of stationary phase compare
Pf1N1B4_5871 +0.6 1.9 FIG00963848: hypothetical protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N1B4 in stress experiments

For stress 1-ethyl-3-methylimidazolium lysine across organisms