Experiment set3IT033 for Herbaspirillum seropedicae SmR1

Sodium nitrate nitrogen source; high aeration

Group: nitrogen source
Media: NFbHP_noNitrogen + Sodium nitrate (10 mM), pH=6.5
Culturing: HerbieS_ML4, flask, Aerobic, at 30 (C), shaken=120 rpm
Growth: about 5.5 generations
By: Thalita Tuleski on 19-May-16

Specific Phenotypes

For 11 genes in this experiment

For nitrogen source Sodium nitrate in Herbaspirillum seropedicae SmR1

For nitrogen source Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	6
Molybdenum cofactor biosynthesis	2
Ammonia assimilation	1
YeiH	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Puromycin biosynthesis
• Lipopolysaccharide biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bis(guanylyl molybdenum cofactor) biosynthesis	2	2	2
guanylyl molybdenum cofactor biosynthesis	1	1	1
bis(guanylyl tungstenpterin) cofactor biosynthesis	1	1	1
alkylnitronates degradation	2	2	1
nitrate reduction V (assimilatory)	2	1	1
molybdopterin biosynthesis	6	3	1