Experiment set3IT029 for Pseudomonas fluorescens FW300-N1B4
LB with Choline chloride 200 mM
Group: stressMedia: LB + Choline chloride (200 mM)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Mark on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1041 D5,D6
Specific Phenotypes
For 6 genes in this experiment
For stress Choline chloride in Pseudomonas fluorescens FW300-N1B4
For stress Choline chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 1 |
| DNA repair, UvrABC system | 1 |
| Peptidoglycan Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nucleotide sugars metabolism
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Puromycin biosynthesis
- Glycine, serine and threonine metabolism
- Glycosaminoglycan degradation
- Lipopolysaccharide biosynthesis
- alpha-Linolenic acid metabolism
- Trinitrotoluene degradation
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| choline degradation I | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |
| queuosine biosynthesis I (de novo) | 4 | 4 | 1 |
| queuosine biosynthesis III (queuosine salvage) | 5 | 3 | 1 |
| peptidoglycan recycling II | 10 | 9 | 1 |
| peptidoglycan recycling I | 14 | 11 | 1 |