Experiment set3IT027 for Pseudomonas fluorescens FW300-N1B4

LB with Choline Lysine 6 mM

Group: stress
Media: LB + Choline Lysine (6 mM)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Mark on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1041 B5,B6

Specific Phenotypes

For 2 genes in this experiment

For stress Choline Lysine in Pseudomonas fluorescens FW300-N1B4

For stress Choline Lysine across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	1
Flagellar motility	1
Rhamnose containing glycans	1
Two-component regulatory systems in Campylobacter	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
dTDP-β-L-rhamnose biosynthesis	5	5	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1