Experiment set3IT026 for Synechococcus elongatus PCC 7942
Sodium nitrite 10 mM
Group: stressMedia: BG11 + Sodium nitrite (10 mM)
Culturing: SynE_ML6, 12 well microplate, Aerobic, at 30 (C), shaken=0 rpm
Growth: about 4.5 generations
By: Kelly on 6/20/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 4 genes in this experiment
For stress Sodium nitrite in Synechococcus elongatus PCC 7942
For stress Sodium nitrite across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Calvin-Benson cycle | 1 |
| Pentose phosphate pathway | 1 |
| Synechocystis experimental | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Carbon fixation in photosynthetic organisms
- Biosynthesis of ansamycins
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pentose phosphate pathway (partial) | 3 | 3 | 2 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 2 |
| pentose phosphate pathway (non-oxidative branch) II | 6 | 5 | 2 |
| plastoquinol-9 biosynthesis I | 3 | 1 | 1 |
| pentose phosphate pathway | 8 | 8 | 2 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 7 | 2 |
| Rubisco shunt | 10 | 10 | 2 |
| superpathway of plastoquinol biosynthesis | 5 | 3 | 1 |
| plastoquinol-9 biosynthesis II | 5 | 3 | 1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 10 | 2 |
| Calvin-Benson-Bassham cycle | 13 | 13 | 2 |
| vitamin E biosynthesis (tocopherols) | 7 | 1 | 1 |
| oxygenic photosynthesis | 17 | 17 | 2 |
| superpathway of glucose and xylose degradation | 17 | 14 | 2 |
| ethene biosynthesis V (engineered) | 25 | 24 | 2 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 21 | 2 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 21 | 2 |
| Bifidobacterium shunt | 15 | 14 | 1 |