Experiment set3IT023 for Pseudomonas fluorescens FW300-N1B4

LB with methylglyoxal 0.008 vol%

Group: stress
Media: LB + methylglyoxal (0.008 vol%)
Culturing: pseudo1_N1B4_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 26 (C), shaken=orbital
By: Mark on 12/16/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1040 B1,B2

Specific Phenotypes

For 7 genes in this experiment

For stress methylglyoxal in Pseudomonas fluorescens FW300-N1B4

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	1
Purine conversions	1
TCA Cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Citrate cycle (TCA cycle)
• Purine metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
TCA cycle VIII (Chlamydia)	6	4	1
incomplete reductive TCA cycle	7	3	1
pyruvate fermentation to propanoate I	7	1	1
partial TCA cycle (obligate autotrophs)	8	8	1
TCA cycle VII (acetate-producers)	9	7	1
TCA cycle V (2-oxoglutarate synthase)	9	7	1
TCA cycle VI (Helicobacter)	9	7	1
TCA cycle IV (2-oxoglutarate decarboxylase)	9	7	1
TCA cycle II (plants and fungi)	9	6	1
TCA cycle I (prokaryotic)	10	8	1
TCA cycle III (animals)	10	6	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	4	1
reductive TCA cycle I	11	6	1
L-glutamate degradation VIII (to propanoate)	11	1	1
superpathway of glyoxylate bypass and TCA	12	10	1
reductive TCA cycle II	12	5	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	5	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
mixed acid fermentation	16	12	1
superpathway of anaerobic energy metabolism (invertebrates)	17	8	1
methylaspartate cycle	19	9	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	17	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	21	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	1